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In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts

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A Correction to this article was published on 30 November 2023

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Abstract

Skin aging is a complex biological process induced by intrinsic and extrinsic factors which is characterized by clinical and cellular changes, especially dermal fibroblasts. It is possible that, some procedures, such as low-level laser therapy (LLLT), could decelerate this process. To test this hypothesis, this study evaluated the in vitro LLLT on dermal fibroblast cell line (HFF-1) with premature senescence H2O2-induced. HFF-1 cells were cultured in standardized conditions, and initially H2O2 exposed at different concentrations. Fibroblasts were also just exposed at different LLLT (660 nm) doses. From these curves, the lowest H2O2 concentration that induced indicators of premature senescence and the lowest LLLT doses that triggered fibroblast proliferation were used in all assays. Cellular mortality, proliferation, and the levels of oxidative, inflammatory cytokines, apoptotic markers, and of two growth signaling molecules (FGF-1 and KGF) were compared among treatments. The H2O2 at 50 μM concentration induced some fibroblast senescence markers and for LLLT, the best dose for treatment was 4 J (p < 0.001). The interaction between H2O2 at 50 μM and LLLT at 4 J showed partially reversion of the higher levels of DNA oxidation, CASP 3, CASP 8, IL-1B, IL-6, and INFy induced by H2O2 exposure. LLLT also trigger increase of IL-10 anti-inflammatory cytokine, FGF-1 and KGF levels. Cellular proliferation was also improved when fibroblasts treated with H2O2 were exposed to LLLT (p < 0.001). These results suggest that in fibroblast with some senescence characteristics H2O2-induced, the LLLT presented an important protective and proliferative action, reverting partially or totally negative effects triggering by H2O2.

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Author notes

  1. Daíse Raquel Maldaner and Verônica Farina Azzolin have contributed equally to produce the present study

Authors and Affiliations

  1. Postgraduate Program of Pharmacology, Federal University of Santa Maria (UFSM), Av. Roraima, 1000, Santa Maria, 97105-900, Brazil

    Daíse Raquel Maldaner, Cibele Ferreira Teixeira, Thiago Duarte, Neida Luiza Pellenz, Ivana Beatrice Mânica da Cruz & Marta Maria Medeiros Frescura Duarte

  2. Postgraduate Program of Gerontology, Federal University of Santa Maria (UFSM), Av. Roraima, 1000, Santa Maria, 97105-900, Brazil

    Verônica Farina Azzolin, Fernanda Barbisan, Moisés Henrique Mastella, Luiz Fernando Cuozzo Lemos & Ivana Beatrice Mânica da Cruz

  3. Biogenomic Laboratory, Federal University of Santa Maria (UFSM), Av. Roraima, 1000, Santa Maria, 97105-900, Brazil

    Alexandre Dihel

  4. Lutheran University of Brazil, BR 287, Km 252, Santa Maria, 97020-970, Brazil

    Carla Maria Uggeri Negretto & Marta Maria Medeiros Frescura Duarte

Authors
  1. Daíse Raquel Maldaner
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  2. Verônica Farina Azzolin
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  3. Fernanda Barbisan
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Correspondence to Fernanda Barbisan.

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Since the study used commercial cell lines, it is not necessary to submit to an ethics committee.

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The original version of this article was revised: This article was originally published with an incorrect author name: Moisés Henrique Mastela. The correct information for the author's name is Moisés Henrique Mastella.

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Maldaner, D.R., Azzolin, V.F., Barbisan, F. et al. In vitro effect of low-level laser therapy on the proliferative, apoptosis modulation, and oxi-inflammatory markers of premature-senescent hydrogen peroxide-induced dermal fibroblasts. Lasers Med Sci 34, 1333–1343 (2019). https://doi.org/10.1007/s10103-019-02728-1

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