Abstract
To investigate the effect of photobiomodulation (PBM) and placebo on total amount of substance P in gingival crevicular fluid (GCF) pre- and postoperatively. Twenty-six patients having tooth with symptomatic apical periodontitis were enrolled in this study. GCF was collected preoperatively. The patients were assigned into two groups (n = 13), as follows: placebo and PBM. Sampling was repeated 7 days after root canal treatment. Two independent samples T test was used for analyzing of the differences between preoperative and postoperative substance P levels in GCF (p = .05). The Pearson correlation analysis was used for determination of correlation among substance P levels and other variables. For placebo group, there is no significant difference between preoperative and postoperative total amounts of substance P level (p = 0.553). For PBM group, postoperative total amount of substance P level was significantly higher than those of preoperative level (p = 0.005). Within the limitation of the present study, PBM has immunomodulation effect linked to the modulation of the total amount of substance P in the gingival crevicular fluid. Thai Clinical Trials Registry: TCTR20161228002
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Payer M, Jakse N, Pertl C, Truschnegg A, Lechner E, Eskici A (2005) The clinical effect of LLLT in endodontic surgery: a prospective study on 72 cases. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 100:375–379. https://doi.org/10.1016/j.tripleo.2004.10.006
Aras MH, Gungormus M (2009) The effect of low-level laser therapy on trismus and facial swelling following surgical extraction of a lower third molar. Photomed Laser Surg 27:21–24. https://doi.org/10.1089/pho.2008.2258
Abreu ME, Viegas VN, Pagnoncelli RM et al (2010) Infrared laser therapy after surgically assisted rapid palatal expansion to diminish pain and accelerate bone healing. World J Orthod 11:273–277
Fikackova H, Dostalova T, Navratil L, Klaschka J (2007) Effectiveness of low-level laser therapy in temporomandibular joint disorders: a placebo-controlled study. Photomed Laser Surg 25:297–303. https://doi.org/10.1089/pho.2007.2053
Noda M, Aoki A, Mizutani K et al (2016) High-frequency pulsed low-level diode laser therapy accelerates wound healing of tooth extraction socket: an in vivo study. Lasers Surg Med. https://doi.org/10.1002/lsm.22560
Bhardwaj S, George JP, Remigus D, Khanna D (2016) Low level laser therapy in the treatment of intra-osseous defect—a case report. J Clin Diagn Res 10:ZD06–ZF08. https://doi.org/10.7860/jcdr/2016/15805.7466
Karu T (2010) Mitochondrial mechanisms of photobiomodulation in context of new data about multiple roles of ATP. Photomed Laser Surg 28:159–160. https://doi.org/10.1089/pho.2010.2789
Antunes F, Boveris A, Cadenas E (2004) On the mechanism and biology of cytochrome oxidase inhibition by nitric oxide. Proc Natl Acad Sci U S A 101:16774–16779. https://doi.org/10.1073/pnas.0405368101
Lim W, Kim J, Kim S et al (2013) Modulation of lipopolysaccharide-induced NF-kappaB signaling pathway by 635 nm irradiation via heat shock protein 27 in human gingival fibroblast cells. Photochem Photobiol 89:199–207. https://doi.org/10.1111/j.1751-1097.2012.01225.x
de Lima FM, Albertini R, Dantas Y et al (2013) Low-level laser therapy restores the oxidative stress balance in acute lung injury induced by gut ischemia and reperfusion. Photochem Photobiol 89:179–188. https://doi.org/10.1111/j.1751-1097.2012.01214.x
Gibran NS, Jang YC, Isik FF et al (2002) Diminished neuropeptide levels contribute to the impaired cutaneous healing response associated with diabetes mellitus. J Surg Res 108:122–128
Richardson JD, Vasko MR (2002) Cellular mechanisms of neurogenic inflammation. J Pharmacol Exp Ther 302:839–845. https://doi.org/10.1124/jpet.102.032797
Harrison S, Geppetti P (2001) Substance P. Int J Biochem Cell Biol 33:555–576
Snijdelaar DG, Dirksen R, Slappendel R, Crul BJ (2000) Substance P. Eur J Pain 4:121–135. https://doi.org/10.1053/eujp.2000.0171
Siqueira JF Jr, Rocas IN, Favieri A et al (2002) Incidence of postoperative pain after intracanal procedures based on an antimicrobial strategy. J Endod 28:457–460. https://doi.org/10.1097/00004770-200206000-00010
Park CK, Bae JH, Kim HY et al (2010) Substance P sensitizes P2X3 in nociceptive trigeminal neurons. J Dent Res 89:1154–1159. https://doi.org/10.1177/0022034510377094
Vishwanath R, Mukherjee R (1996) Substance P promotes lymphocyte-endothelial cell adhesion preferentially via LFA-1/ICAM-1 interactions. J Neuroimmunol 71:163–171
Kant V, Gopal A, Kumar D et al (2013) Topically applied substance P enhanced healing of open excision wound in rats. Eur J Pharmacol 715:345–353. https://doi.org/10.1016/j.ejphar.2013.04.042
Benrath J, Zimmermann M, Gillardon F (1995) Substance P and nitric oxide mediate would healing of ultraviolet photodamaged rat skin: evidence for an effect of nitric oxide on keratinocyte proliferation. Neurosci Lett 200:17–20
Burssens P, Steyaert A, Forsyth R, van Ovost EJ, Depaepe Y, Verdonk R (2005) Exogenously administered substance P and neutral endopeptidase inhibitors stimulate fibroblast proliferation, angiogenesis and collagen organization during Achilles tendon healing. Foot Ankle Int 26:832–839
Levrini L, Sacerdote P, Moretti S, Panzi S, Caprioglio A (2013) Changes of substance P in the crevicular fluid in relation to orthodontic movement preliminary investigation. Scientific World Journal 2013:896874. https://doi.org/10.1155/2013/896874
Chapple IL, Cross IA, Glenwright HD, Matthews JB (1995) Calibration and reliability of the Periotron 6000 for individual gingival crevicular fluid samples. J Periodontal Res 30:73–79
Khabbaz MG, Protogerou E, Douka E (2010) Radiographic quality of root fillings performed by undergraduate students. Int Endod J 43:499–508. https://doi.org/10.1111/j.1365-2591.2010.01706.x
Yoo YJ, Shon WJ, Baek SH, Kang MK, Kim HC, Lee W (2014) Effect of 1440-nanometer neodymium:yttrium-aluminum-garnet laser irradiation on pain and neuropeptide reduction: a randomized prospective clinical trial. J Endod 40:28–32. https://doi.org/10.1016/j.joen.2013.07.011
Shin SJ, Lee W, Lee JI et al (2011) Matrix metalloproteinase-8 and substance P levels in gingival crevicular fluid during endodontic treatment of painful, nonvital teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112:548–554. https://doi.org/10.1016/j.tripleo.2011.04.026
Awawdeh LA, Lundy FT, Linden GJ, Shaw C, Kennedy JG, Lamey PJ (2002) Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in gingival crevicular fluid associated with painful human teeth. Eur J Oral Sci 110:185–191
Caviedes-Bucheli J, Muñoz HR, Azuero-Holguín MM, Ulate E (2008) Neuropeptides in dental pulp: the silent protagonists. J Endod 34:773–788
Roosterman D, Goerge T, Schneider SW, Bunnett NW, Steinhoff M (2006) Neuronal control of skin function: the skin as a neuroimmunoendocrine organ. Physiol Rev 86:1309–1379
Schmelz M, Petersen LJ (2001) Neurogenic inflammation in human and rodent skin. Physiology 16:33–37
Steinhoff M, Ständer S, Seeliger S, Ansel JC, Schmelz M, Luger T (2003) Modern aspects of cutaneous neurogenic inflammation. Arch Dermatol 139:1479–1488
Theoharides TC, Singh KL, Boucher W et al (1998) Corticotropin-releasing hormone induces skin mast cell degranulation and increased vascular permeability, a possible explanation for its proinflammatory effects 1. Endocrinology 139:403–413
Hong HS, Lee J, Lee E et al (2009) A new role of substance P as an injury-inducible messenger for mobilization of CD29+ stromal-like cells. Nat Med 15:425–435
Pamuk F, Lutfioglu M, Aydogdu A, Koyuncuoglu CZ, Cifcibasi E, Badur OS (2017) The effect of low-level laser therapy as an adjunct to non-surgical periodontal treatment on gingival crevicular fluid levels of transforming growth factor-beta 1, tissue plasminogen activator and plasminogen activator inhibitor 1 in smoking and non-smoking chronic periodontitis patients: a split-mouth, randomized control study. J Periodontal Res 52:872–882. https://doi.org/10.1111/jre.12457
Kumaresan D, Balasundaram A, Naik VK, Appukuttan DP (2016) Gingival crevicular fluid periostin levels in chronic periodontitis patients following nonsurgical periodontal treatment with low-level laser therapy. Eur J Dent 10:546–550. https://doi.org/10.4103/1305-7456.195179
Hochman B, Pinfildi CE, Nishioka MA et al (2014) Low-level laser therapy and light-emitting diode effects in the secretion of neuropeptides SP and CGRP in rat skin. Lasers Med Sci 29:1203–1208. https://doi.org/10.1007/s10103-013-1494-z
Hsieh YL, Hong CZ, Chou LW, Yang SA, Yang CC (2015) Fluence-dependent effects of low-level laser therapy in myofascial trigger spots on modulation of biochemicals associated with pain in a rabbit model. Lasers Med Sci 30:209–216. https://doi.org/10.1007/s10103-014-1654-9
Caviedes-Bucheli J, Moreno JO, Carreno CP et al (2013) The effect of single-file reciprocating systems on substance P and calcitonin gene-related peptide expression in human periodontal ligament. Int Endod J 46:419–426. https://doi.org/10.1111/iej.12005
Seltzer S, Bender IB (1984) The dental pulp: biologic considerations in dental procedures, 3rd edn. Lippincott Williams & Wilkins, Philadelphia Pages
Ghazi AM, Shuttleworth S, Angulo SJ, Pashley DH (2000) Gallium diffusion in human root dentin: quantitative measurements by pulsed Nd:YAG laser ablation combined with an inductively coupled plasma mass spectrometer. J Clin Laser Med Surg 18:173–183. https://doi.org/10.1089/10445470050144029
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The work was supported by the Department of Endodontics, Erzurum, Turkey.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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Doğanay Yıldız, E., Arslan, H., Köseoğlu, S. et al. The effect of photobiomodulation on total amount of substance P in gingival crevicular fluid: placebo-controlled randomized clinical trial. Lasers Med Sci 34, 517–523 (2019). https://doi.org/10.1007/s10103-018-2625-3
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DOI: https://doi.org/10.1007/s10103-018-2625-3