Abstract
Amphiphysin 1 (AMPH-1) is a nerve terminal-enriched protein and it is a 128-kD protein with three identified functional domains. Some studies found that AMPH-1 was a dominant autoantigen associated with breast cancer and melanoma. However, its function in lung cancer is unknown. Here, we showed that AMPH-1 knockdown dramatically increased cell proliferation, attenuated cell apoptosis, and promoted cell cycle progression in human lung cancer cells. In vivo xenograft studies confirmed that the AMPH-1-knockdown cells were more tumorigenic than the controls. Moreover, we demonstrated that silencing AMPH-1 markedly activated Ras-Raf-MEK-ERK signal pathway. In summary, our results identified the anti-oncogenic function of AMPH-1 in lung cancer in vitro and in vivo. It is proposed that AMPH-1 may have potential as a new therapeutic target in human lung cancer treatment.
Similar content being viewed by others
References
Sharma SV, Bell DW, Settleman J, Haber DA (2007) Epidermal growth factor receptor mutations in lung cancer. Nat Rev Cancer 7:169–181
Brambilla E, Travis WD, Colby TV, Corrin B, Shimosato Y (2001) The new World Health Organization classification of lung tumours. Eur Respir J 18:1059–1068
Mills NE, Fishman CL, Rom WN, Dubin N, Jacobson DR (1995) Increased prevalence of K-ras oncogene mutations in lung adenocarcinoma. Cancer Res 55:1444–1447
Reynolds SH, Anna CK, Brown KC et al (1991) Activated protooncogenes in human lung tumors from smokers. Proc Natl Acad Sci U S A 88:1085–1089
Mitsudomi T, Viallet J, Mulshine JL, Linnoila RI, Minna JD, Gazdar AF (1991) Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines. Oncogene 6:1353–1362
Fisher GH, Wellen SL, Klimstra D et al (2001) Induction and apoptotic regression of lung adenocarcinomas by regulation of a K-Ras transgene in the presence and absence of tumor suppressor genes. Genes Dev 15:3249–3262
Guerra C, Mijimolle N, Dhawahir A et al (2003) Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context. Cancer Cell 4:111–120
Jackson EL, Willis N, Mercer K et al (2001) Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Genes Dev 15:3243–3248
Ji H, Houghton AM, Mariani TJ et al (2006) K-ras activation generates an inflammatory response in lung tumors. Oncogene 25:2105–2112
Johnson L, Mercer K, Greenbaum D et al (2001) Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature 410:1111–1116
Farago AF, Snyder EL, Jacks T (2012) SnapShot: lung cancer models. Cell 149:246–246 e241
Molina JR, Yang P, Cassivi SD, Schild SE, Adjei AA (2008) Non-small cell lung cancer: epidemiology, risk factors, treatment, and survivorship. Mayo Clinic Proc 83:584–594
Mehlen P, Puisieux A (2006) Metastasis: a question of life or death. Nat Rev Cancer 6:449–458
Mitsuuchi Y, Testa JR (2002) Cytogenetics and molecular genetics of lung cancer. Am J Med Genet 115:183–188
Ding L, Getz G, Wheeler DA et al (2008) Somatic mutations affect key pathways in lung adenocarcinoma. Nature 455:1069–1075
Weir BA, Woo MS, Getz G et al (2007) Characterizing the cancer genome in lung adenocarcinoma. Nature 450:893–898
Pittock SJ, Lucchinetti CF, Parisi JE et al (2005) Amphiphysin autoimmunity: paraneoplastic accompaniments. Ann Neurol 58:96–107
David C, McPherson PS, Mundigl O, de Camilli P (1996) A role of amphiphysin in synaptic vesicle endocytosis suggested by its binding to dynamin in nerve terminals. Proc Natl Acad Sci U S A 93:331–335
Wigge P, Kohler K, Vallis Y et al (1997) Amphiphysin heterodimers: potential role in clathrin-mediated endocytosis. Mol Biol Cell 8:2003–2015
Slepnev VI, Ochoa GC, Butler MH, De Camilli P (2000) Tandem arrangement of the clathrin and AP-2 binding domains in amphiphysin 1 and disruption of clathrin coat function by amphiphysin fragments comprising these sites. J Biol Chem 275:17583–17589
De Jesus-Cortes HJ, Nogueras-Ortiz CJ, Gearing M, Arnold SE, Vega IE (2012) Amphiphysin-1 protein level changes associated with tau-mediated neurodegeneration. Neuroreport 23:942–946
Sekiguchi M, Katayama S, Hatano N, Shigeri Y, Sueyoshi N, Kameshita I (2013) Identification of amphiphysin 1 as an endogenous substrate for CDKL5, a protein kinase associated with X-linked neurodevelopmental disorder. Arch Biochem Biophys 535:257–267
Floyd SR, Porro EB, Slepnev VI, Ochoa GC, Tsai LH, De Camilli P (2001) Amphiphysin 1 binds the cyclin-dependent kinase (cdk) 5 regulatory subunit p35 and is phosphorylated by cdk5 and cdc2. J Biol Chem 276:8104–8110
Wu Y, Matsui H, Tomizawa K (2009) Amphiphysin I and regulation of synaptic vesicle endocytosis. Acta Med Okayama 63:305–323
Takei K, Slepnev VI, Haucke V, De Camilli P (1999) Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis. Nat Cell Biol 1:33–39
Folli F, Solimena M, Cofiell R et al (1993) Autoantibodies to a 128-kd synaptic protein in three women with the stiff-man syndrome and breast cancer. N Engl J Med 328:546–551
De Camilli P, Thomas A, Cofiell R et al (1993) The synaptic vesicle-associated protein amphiphysin is the 128-kD autoantigen of Stiff-Man syndrome with breast cancer. J Exp Med 178:2219–2223
Schmierer K, Grosse P, De Camilli P, Solimena M, Floyd S, Zschenderlein R (2002) Paraneoplastic stiff-person syndrome: no tumor progression over 5 years. Neurology 58:148
Crouzet M, Urdaci M, Dulau L, Aigle M (1991) Yeast mutant affected for viability upon nutrient starvation: characterization and cloning of the RVS161 gene. Yeast 7:727–743
Bauer F, Urdaci M, Aigle M, Crouzet M (1993) Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns. Mol Cell Biol 13:5070–5084
Dropcho EJ (1996) Antiamphiphysin antibodies with small-cell lung carcinoma and paraneoplastic encephalomyelitis. Ann Neurol 39:659–667
Dalmau J, Graus F, Rosenblum MK, Posner JB (1992) Anti-Hu-associated paraneoplastic encephalomyelitis/sensory neuronopathy. A clinical study of 71 patients. Medicine (Baltimore) 71:59–72
Otsuka A, Hirose K, Kilimann MW, Kamata T (2003) Amphiphysin1 inhibits vitronectin-mediated cell adhesion, spreading, and migration in vitro. Biochem Biophys Res Commun 301:769–775
Karreth FA, Frese KK, DeNicola GM, Baccarini M, Tuveson DA (2011) C-Raf is required for the initiation of lung cancer by K-Ras (G12D). Cancer Discov 1:128–136
Janku F, Lee JJ, Tsimberidou AM et al (2011) PIK3CA mutations frequently coexist with RAS and BRAF mutations in patients with advanced cancers. PLoS One 6:e22769
Funding
This work was sponsored by Qing Lan Project in Jiangsu Province of China. This work was also supported by the National Natural Science Foundation of China (No.81772856, 81,402,222) and Youth Fund of Shanghai Municipal Health Planning Commission (No.2017YQ054, 2017Y0117).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
All experimental protocols and methods were approved by the Jiangsu Agri-Animal Husbandry Vocational College. We also confirmed that all methods were performed in accordance with the relevant guidelines and regulations. Mice were bred in the Animal Core Facility by following procedures approved by the Jiangsu Agri-Animal Husbandry Vocational College of Institutional Animal Care and Use Committee.
Rights and permissions
About this article
Cite this article
Yang, H., Wan, Z., Huang, C. et al. AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway. Lasers Med Sci 34, 473–478 (2019). https://doi.org/10.1007/s10103-018-2616-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10103-018-2616-4