The impact of photobiomodulation on osteoblast-like cell: a review
In this study, we present a review of the literature on the impact of photobiomodulation on osteoblast-like cell culture. Searches were performed in the PubMed/MEDLINE (Medical Literature Analysis and Retrieval System Online), SCOPUS, and SPIE digital library databases for original articles regarding the effects of LLLT on osteoblast-like cells in experimental models using LLLT published in English from the last 20 years. The search identified 1439 studies. After the analysis of the abstracts, 1409 studies were excluded and 30 studies were then selected for the full-text analysis, 8 of which were excluded. Thus, 22 studies were included for a critical evaluation of the impact of photobiomodulation on osteoblast-like cell culture. The cell lineages studied were primary rat, primary human, saos-2, Osteo-1, MC3T3, MG63, and OFCOL II. Moreover, a wide variety of experimental models were used to experimentally analyze the impact of photobiomodulation, the most common of which were alkaline phosphatase, MTT, and cell count. This review suggests that osteoblastic-like cells are susceptible to photobiomodulation but that most of the light parameters varied by different authors have little to no influence on proliferation but very high levels of irradiance have demonstrated deleterious effects on proliferation, highlighting the bi-phasic effect of photobiomodulation.
KeywordsPhotobiomodulation; laser LED Osteoblast Review
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This work is a review; therefore, no ethical committee approval is required.
This work is a review; therefore, no informed consent was necessary.
- 3.Asai T, Suzuki H, Kitayama M, Matsumoto K, Kimoto A, Shigeoka M, Komori T (2014) The long-term effects of red light-emitting diode irradiation on the proliferation and differentiation of osteoblast-like MC3T3-E1 cells. J Med Sci 60(1):E12-E18Google Scholar
- 20.Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, Thurnher D, Baghestanian M, Turhani D (2008) Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr 120(3):112–117. https://doi.org/10.1007/s00508-008-0932-6 CrossRefPubMedGoogle Scholar
- 21.Bloise N, Ceccarelli G, Minzioni P, Vercellino M, Benedetti L, MGC DA, Imbriani M et al (2013) Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors. J Biomed Opt 18(12):128006CrossRefPubMedGoogle Scholar
- 24.Emes Y, Akça K, Aybar B, Yalçın S, Çavuşoğlu Y, Baysal U, Işsever H, Atalay B, Vural P, Ergüven M, Çehrel MC, Bilir A (2013) Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells. Lasers Med Sci 28:901–909. https://doi.org/10.1007/s10103-012-1165-5 CrossRefPubMedGoogle Scholar
- 27.Silva APRB, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AD, Stuani MBS (2012) Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci 27:777–783. https://doi.org/10.1007/s10103-011-0968-0 CrossRefPubMedGoogle Scholar
- 29.Danti S, Serino LP, D’Alessandro D, Moscato S, Danti S, Trombi L, Dinucci D, Chiellini F, Pietrabissa A, Lisanti M, Berrettini S, Petrini M (2013) Growing bone tissue-engineered niches with graded osteogenicity: an in vitro method for biomimetic construct assembly. Tissue Eng Part C Methods 19(12). https://doi.org/10.1089/ten.tec.2012.0445
- 34.Ma WH, Liu YJ, Wang W, Zhang YZ (2015) Neuropeptide Y, substance P, and human boné morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication. Braz J Med Biol Res 48(4):299–307. https://doi.org/10.1590/1414-431X20144226 ISSN 1414-431X