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Prevalence of fosfomycin resistance among ESBL-producing Escherichia coli isolates in the community, Switzerland

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Abstract

Our aim was to evaluate the prevalence of fosfomycin-resistant strains among ESBL-producing Escherichia coli isolates recovered from community patients in Switzerland. A total of 1225 ESBL-producing E. coli isolates were collected between 2012 and 2013 from a private and community laboratory. Fosfomycin resistance was assessed by using the novel rapid fosfomycin/E. coli NP test and agar dilution method. Resistant isolates were further investigated for acquired resistance genes fosA1–7 by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to evaluate the clonal relationship among fosA3-carrying isolates. Out of the 1225 ESBL-producing E. coli isolates analyzed in this study, 1208 were fosfomycin susceptible while 17 were fosfomycin resistant. No discrepancy was observed between the rapid fosfomycin/E. coli NP test and the agar dilution method taken as the gold standard. Five out of the 17 resistant isolates carried a fosA-like gene. No clonal relationship was observed among those isolates. Here, the prevalence of fosfomycin resistance among ESBL-producing E. coli isolates in the community is reported for the first time in Switzerland, being ca. 1.4%. Among the five isolates carrying a fosA gene, four encoded the FosA3 enzyme, being the most prevalent fosfomycin-resistant determinant. An excellent correlation was observed between minimum inhibitory concentration–based susceptibility categorization and results of the rapid fosfomycin/E. coli NP test, further indicating the excellent sensitivity and specificity of this recently developed rapid test whose results are obtained in less than 2 h.

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Funding

This work was funded by the Swiss National Science Foundation (project FNS-407240_177382).

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Authors and Affiliations

  1. Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, 1700, Fribourg, Switzerland

    Linda Mueller, Cansu Cimen, Laurent Poirel & Patrice Nordmann

  2. Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland

    Linda Mueller, Laurent Poirel & Patrice Nordmann

  3. Infectious Diseases and Clinical Microbiology Clinic, Ardahan Public Hospital, Ardahan, Turkey

    Cansu Cimen

  4. INSERM European Unit (IAME/LEA, France), University of Fribourg, Fribourg, Switzerland

    Laurent Poirel & Patrice Nordmann

  5. Laboratoire Proxilis, Meyrin, Switzerland

    Marie-Christine Descombes

  6. University Hospital Center and University of Lausanne, Lausanne, Switzerland

    Patrice Nordmann

Authors
  1. Linda Mueller
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  2. Cansu Cimen
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  3. Laurent Poirel
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  4. Marie-Christine Descombes
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  5. Patrice Nordmann
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Correspondence to Linda Mueller.

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Mueller, L., Cimen, C., Poirel, L. et al. Prevalence of fosfomycin resistance among ESBL-producing Escherichia coli isolates in the community, Switzerland. Eur J Clin Microbiol Infect Dis 38, 945–949 (2019). https://doi.org/10.1007/s10096-019-03531-0

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  • DOI: https://doi.org/10.1007/s10096-019-03531-0

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