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Comparison of three multiplex real-time PCR assays for detection of enteric viruses in patients with diarrhea

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Abstract

In this study, the usability and performance of three commercially available multiplex real-time RT-PCR assays for the detection of major enteric viruses were investigated. In total, 481 fecal specimens were analyzed using the Allplex™ GI Virus Assay, the Rida®Gene Viral Stool Panel I, and the FTD Viral Gastroenteritis. The overall agreement between the assays was 99.9%. Despite convergent analytical performance, differences between the multiplex RT-PCR assays were apparent when considering their suitability for routine diagnostics.

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Acknowledgements

Siru Koskinen, Linda Luoma, and Hanna Saari are gratefully acknowledged for their help in sample analyses.

Funding

This study was carried out alongside routine diagnostics.

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Authors and Affiliations

  1. Fimlab Laboratories, Arvo Ylpön katu 4, FI-33520, Tampere, Finland

    Jari J. Hirvonen

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  1. Jari J. Hirvonen
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Correspondence to Jari J. Hirvonen.

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The author reports no conflict of interests.

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This article does not contain any studies with human participants or animals performed by the author. There were no requirements for informed consent.

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Hirvonen, J.J. Comparison of three multiplex real-time PCR assays for detection of enteric viruses in patients with diarrhea. Eur J Clin Microbiol Infect Dis 38, 241–244 (2019). https://doi.org/10.1007/s10096-018-3418-0

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  • DOI: https://doi.org/10.1007/s10096-018-3418-0

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