Neurological Sciences

, Volume 39, Issue 4, pp 647–654 | Cite as

Peripheral Th17/Treg imbalance in elderly patients with ischemic stroke

  • Sanam Dolati
  • Majid Ahmadi
  • Mohammad Khalili
  • Ali Akabar Taheraghdam
  • Homayoon Siahmansouri
  • Zohreh Babaloo
  • Leili Aghebati-Maleki
  • Farhad Jadidi-Niaragh
  • Vahid Younesi
  • Mehdi YousefiEmail author
Original Article


CD4+CD25+ regulatory T (Treg) cells and Th17 cells play important roles in peripheral immunity. Immune responses are main elements in the pathogenesis of ischemic stroke (IS). The contribution of Th17 cells in IS patients has not been proved, and whether the balance of Treg/Th17 cells is changed in IS patients remains unidentified. In the present study, we studied Th17 and Treg cell frequency, cytokine secretion, expression of transcription factors, and microRNAs related to Th17 and Treg cells differentiation, which is compared between IS patients and control group. Thirty patients with IS and 30 individuals as control group were enrolled in this study. The frequency of Th17 and Treg lymphocytes, the expression of transcription factors and microRNAs related to these cells, and the serum levels of associated cytokines were assessed by flow cytometry, real-time PCR, and ELISA, respectively. A significant reduction in proportion of peripheral Treg cell frequency and the levels of TGF-β and FOXP3 expression were observed in patients with IS compared with controls, while the proportions of Th17 were increased dramatically, and these effects were along with increases in the levels of IL-17A and RORγt expression in IS patients. The levels of mir-326 and mir-106b-25 expression were increased in patients with IS. These studies suggest that the increase in proportion of Th17 cells and decrease in Treg cells might contribute to the pathogenesis of IS. Manipulating the balance between Tregs and Th17 cells might be helpful for the treatment of IS.


Ischemic stroke Th17 Treg miRNAs 



This work was financially supported by the grant of Aging Research Institute, Tabriz University of Medical Sciences, and Tabriz, Iran.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.


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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018
corrected publication February/2018

Authors and Affiliations

  • Sanam Dolati
    • 1
    • 2
    • 3
    • 4
  • Majid Ahmadi
    • 1
    • 2
  • Mohammad Khalili
    • 5
  • Ali Akabar Taheraghdam
    • 5
  • Homayoon Siahmansouri
    • 2
    • 4
  • Zohreh Babaloo
    • 4
  • Leili Aghebati-Maleki
    • 6
  • Farhad Jadidi-Niaragh
    • 2
    • 4
  • Vahid Younesi
    • 7
  • Mehdi Yousefi
    • 2
    • 4
    • 8
    Email author
  1. 1.Aging Research InstituteTabriz University of Medical SciencesTabrizIran
  2. 2.Drug Applied Research CenterTabriz University of Medical SciencesTabrizIran
  3. 3.Student’s Research CommitteeTabriz University of Medical SciencesTabrizIran
  4. 4.Department of Immunology, School of MedicineTabriz University of Medical SciencesTabrizIran
  5. 5.Neurosciences Research CenterTabriz University of Medical SciencesTabrizIran
  6. 6.Immunology Research CenterTabriz University of Medical SciencesTabrizIran
  7. 7.Pishtaz Teb Zaman DiagnosticsTehranIran
  8. 8.Department of Immunology, Faculty of MedicineTabriz University of Medical SciencesTabrizIran

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