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Transient elevation of synaptosomal mitoenergetic proteins and Hsp70 early in a rat model of chronic cerebrovascular hypoperfusion

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Abstract

Chronic cerebral hypoperfusion (CCH) might account for the cognitive deficits associated with vascular cognitive impairment, but the mechanisms of hypoperfusion insulting to the cognition remain obscure. In the present study, Wistar rats underwent permanent occlusion of bilateral common carotid arteries to induce CCH. 2D-DIGE combined with MALDI-TOF MS was applied to determine the proteins that were differentially expressed in synaptosomes of prefrontal cortex and hippocampus. ATPsynβ, NDUFS1, UQCRC1 and Hsp70 were elevated both in synaptosomes of cortex and hippocampus at week 2 after operation, but subsided to baseline at week 4 except ATPsynβ which was still upregulated in synaptosomes of hippocampus at week 4. IDH3A and PDC-E2 were increased, respectively, in synaptosomes of prefrontal cortex and hippocampus at week 2, and showed no difference when compared to control at week 4. Malate dehydrogenase showed no difference in synaptosomes of prefrontal cortex and hippocampus at week 2, but showed an elevation in synaptosomes of prefrontal cortex at week 4. Our results imply that metabolic reserve and anti-oxidative stress might transiently exist in the early stage of CCH, which probably help cognitive save.

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Abbreviations

CCH:

Chronic cerebrovascular hypoperfusion

VCI:

Vascular cognitive impairment

2VO:

Permanent occlusion of bilateral common carotid arteries

VaD:

Vascular dementia

MCI:

Mild cognitive impairment

LTP:

Long-term potential

2D-DIGE:

Two-dimensional difference gel electrophoresis

MALDI-TOF MS:

Matrix-assisted laser desorption ionization time of flight mass spectrometry

ATPsynβ:

ATP synthase beta subunit

NDUFS1:

NADH dehydrogenase (ubiquinone) Fe-S protein 1

IDH3A:

Isocitrate dehydrogenase 3 (NAD+) alpha precursor

UQCRC1:

Ubiquinol-cytochrome c reductase core protein 1

PDC-E2:

Dihydrolipoamide acetyltransferase

MDH:

Malate dehydrogenase

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant 30770756).

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Authors and Affiliations

  1. Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, Changchun, 130021, China

    Hai Ning Zhang, Jiang Wu, Tao Jin, Ming Chang & Li Sun

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  1. Hai Ning Zhang
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  2. Jiang Wu
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  3. Tao Jin
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  4. Ming Chang
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  5. Li Sun
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Corresponding author

Correspondence to Li Sun.

Additional information

H. N. Zhang and J. Wu contributed equally to this work.

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Zhang, H.N., Wu, J., Jin, T. et al. Transient elevation of synaptosomal mitoenergetic proteins and Hsp70 early in a rat model of chronic cerebrovascular hypoperfusion. Neurol Sci 34, 471–477 (2013). https://doi.org/10.1007/s10072-012-1063-4

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  • DOI: https://doi.org/10.1007/s10072-012-1063-4

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