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Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae

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Abstract

In order to develop processing methods with high physiological activity for Protaetia brevitarsis larvae (PBL), subcritical water (SCW) extraction was applied. The dried powder (1 g) of PBL was extracted with 10 mL distilled water at 100, 200, and 300 °C for 30 min. The SCW treatment significantly (p < 0.05) increased some physiological activities of the PBL extracts. The SCW extract at 300 °C increased alcohol dehydrogenase, acetaldehyde dehydrogenase, and tyrosinase inhibitory activities from 192.3 ± 4.1% to 452.2 ± 0.5%, 125.4 ± 2.9% to 153.3 ± 0.4%, and − 7.0 ± 0.7% to 26.1 ± 1.4%, respectively, compared to the extract at 100 °C. Contrarily, the inhibition activity of angiotensin converting enzyme was the highest at 200 °C. These results suggest that SCW is a suitable method to extract and maintain the physiological activity of PBL.

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Acknowledgements

This work was supported by Kyungnam University Foundation Grant, 2019.

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Authors and Affiliations

  1. Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research and Extension Services, Namgang-ro 1851, Jinju, 52638, Republic of Korea

    Sung-Mun Bae

  2. Department of Food Development, School of Bioconvergence, College of Health Sciences, Kyungnam University, Kyungnamdaehak-ro 7, Changwon, 51767, Republic of Korea

    Seung-Cheol Lee

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  1. Sung-Mun Bae
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Bae, SM., Lee, SC. Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae. Food Sci Biotechnol 29, 867–872 (2020). https://doi.org/10.1007/s10068-019-00728-z

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