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Disruption of Phaffia rhodozyma cells and preparation of microencapsulated astaxanthin with high water solubility

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Abstract

A novel process was developed for encapsulation of astaxanthin from Phaffia rhodozyma. The yeast cells were disrupted by glass beads and the high shearing force partially emulsified the astaxanthin in aqueous phase. The enzymolysis method was then adopted to prepare the yeast extract for a full use of the cells. The gelatin and porous starch were used to microencapsulate the emulsified astaxanthin. Under optimized conditions, the recovery of amino nitrogen and solid reached 3.68 ± 0.32% and 49.22 ± 2.34%, respectively. The microencapsulation conditions were optimized through orthogonal experiment and the encapsulation efficiency, loading astaxanthin, and amino-nitrogen reached 88.56%, 1.55 mg/g, and 1.35 ± 0.14%, respectively. The water solubility of microcapsules reached 81.5 ± 0.35%. Color and storage stability analysis showed that microencapsulation of astaxanthin possessed higher thermal stability. The results demonstrated that the established process was effective and practical.

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References

  • An GH, Schuman DB, Johnson EA. Isolation of Phaffia rhodozyma mutants with increased astaxanthin content. Appl. Environ. Microb. 55(1):116–124 (1989)

    Article  CAS  Google Scholar 

  • Anarjan N, Tan CP, Nehdi IA, Ling TC. Colloidal astaxanthin: Preparation, characterisation and bioavailability evaluation. Food Chem. 135(3):1303–1309 (2012)

    Article  CAS  Google Scholar 

  • Anderson JS, Sunderland R. Effect of extruder moisture and dryer processing temperature on vitamin C and E and astaxanthin stability. Aquaculture. 207:137–149 (2001)

    Article  Google Scholar 

  • Bellora N, Moliné M, David-Palma M, Coelho MA, Hittinger CT, Sampaio JP, Libkind D. Comparative genomics provides new insights into the diversity, physiology, and sexuality of the only industrially exploited tremellomycete: Phaffia rhodozyma. BMC Genomics. 17:901 (2016)

    Article  Google Scholar 

  • Bhatt PC, Ahmad M, Panda BP. Enhanced bioaccumulation of astaxanthin in Phaffia rhodozyma by utilising low-cost agro products as fermentation substrate. Biocatalysis and Agricultural Biotechnology. 2(1):58–63 (2013)

    Article  Google Scholar 

  • Bie XY, Zhu MJ. Sucrose biotransformation by immobilized Phaffia rhodozyma and continuous neokestose production in a packed-bed reactor. Biocatal. Biotransform. 34(3):89–98 (2016)

    Article  CAS  Google Scholar 

  • Budarin V, Clark JH, Hardy JJE, Luque R, Milkowski K, Tavener SJ, Wilson AJ. Starbons: new starch-derived mesoporous carbonaceous materials with tunable properties. Angew. Chem. 118(23):3866–3870 (2006)

    Article  Google Scholar 

  • Cardozo KHM, Guaratini T, Barros MP, Falcão VR, Tonon AP, Lopes NP. Metabolites from algae with economical impact. Comp. Biochem. Physiol. C. 146(1–2):60–78 (2007)

    Google Scholar 

  • Chen X, Chen R, Guo Z, Li C, Li P. The preparation and stability of the inclusion complex of astaxanthin with β-cyclodextrin. Food Chem. 101(4):1580–1584 (2007)

    Article  CAS  Google Scholar 

  • Chew BP, Mathison BD, Hayek MG, Massimino S, Reinhart GA, Park JS. Dietary astaxanthin enhances immune response in dogs. Vet. Immunol. Immunopathol. 140(3–4):199–206 (2011)

    Article  CAS  Google Scholar 

  • Choi J, Rahman MM, Lee SY, Chang KH, Lee SM. Effects of dietary inclusion of fermented soybean meal with Phaffia rhodozyma on growth, muscle pigmentation, and antioxidant activity of juvenile rainbow trout (Oncorhynchus mykiss). Turk. J. Fish. Aquat Sci. 16(1):091–101 (2016)

    Article  Google Scholar 

  • Comunian TA, Thomazini M, Alves AJG, de Matos Junior FE, de Carvalho Balieiro JC, Favaro-Trindade CS. Microencapsulation of ascorbic acid by complex coacervation: Protection and controlled release. Food Res. Int. 52(1):373–379 (2013)

    Article  CAS  Google Scholar 

  • Evi VO, Balan B, Ak-Cvitanovi AB, Levi S, Trifkovi K. Trends in encapsulation technologies for delivery of food bioactive compounds. Food Eng. Rev. 7:452–490 (2015)

    Article  Google Scholar 

  • Fang TJ, Wang J. Extractability of astaxanthin in a mixed culture of a carotenoid over-producing mutant of Xanthophyllomyces dendrorhous and Bacillus circulans in two-stage batch fermentation. Process Biochem. 37(11):1235–1245 (2002)

    Article  CAS  Google Scholar 

  • Fonseca RAS, Rafael RS, Kalil SJ, Burkert CAV, Burkert JFM. Different cell disruption methods for astaxanthin recovery by Phaffia rhodozyma. Afr. J. Biotechnol. 10(7):1165–1171 (2011)

    Google Scholar 

  • Gomez-Estaca J, Comunian TA, Montero P, Ferro-Furtado R, Favaro-Trindade CS. Encapsulation of an astaxanthin-containing lipid extract from shrimp waste by complex coacervation using a novel gelatin–cashew gum complex. Food Hydrocolloid. 61:155–162 (2016)

    Article  CAS  Google Scholar 

  • Guerin M, Huntley ME, Olaizola M. Haematococcus astaxanthin: applications for human health and nutrition. Trends Biotechnol. 21(5):210–216 (2003)

    Article  CAS  Google Scholar 

  • Jafari SM, He Y, Bhandari B. Encapsulation of nanoparticles of d-limonene by spray drying: role of emulsifiers and emulsifying techniques. Drying Technol. 25(6):1069–1079 (2007)

    Article  CAS  Google Scholar 

  • Jiang GL, Zhou LY, Wang YT, Zhu MJ. Astaxanthin from Jerusalem artichoke: Production by fed-batch fermentation using Phaffia rhodozyma and application in cosmetics. Process Biochem. 63:16–25 (2017)

    Article  CAS  Google Scholar 

  • Lee S, Fang TJ. Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater. Biotechnol. Lett. 33(1):109–112 (2011)

    Article  CAS  Google Scholar 

  • Leite MF, De Lima A, Massuyama MM, Otton R. In vivo astaxanthin treatment partially prevents antioxidant alterations in dental pulp from alloxan-induced diabetic rats. Int. Endod. J. 43(11):959–967 (2010)

    Article  CAS  Google Scholar 

  • Li RW, Cai ZW, Li ZW, Zhang Q, Zhang SY. Synthesis of in situ formable hydrogels with collagen and hyaluronan through facile michael addition. Mat. Sci. Eng. C. 77:1035–1043 (2017)

    Article  CAS  Google Scholar 

  • Liu XJ, Luo QX, Cao Y, Goulette T, Liu X, Xiao H. Mechanism of Different Stereoisomeric Astaxanthin in Resistance to Oxidative Stress in Caenorhabditis elegans. J. Food Sci. 81(9):H2280–H2287 (2016)

    Article  CAS  Google Scholar 

  • Mata-Gómez LC, Monta Ez JC, Méndez-Zavala A, Aguilar CN. Biotechnological production of carotenoids by yeasts: an overview. Microbial Cell Factories. 13:12 (2014)

    Article  Google Scholar 

  • Michelon M, de Borba TDM, da Silva Rafael R, Burkert CAV, de Medeiros Burkert JF. Extraction of carotenoids from Phaffia rhodozyma: A comparison between different techniques of cell disruption. Food Sci. Biotechnol. 21(1):1–8 (2012)

    Article  CAS  Google Scholar 

  • Najafi N, Hosseini R, Ahmadi AR. The effect of gamma irradiation on astaxanthin synthetase encoding gene in two mutant strains of Phaffia rhodozyma. Iran J. Microbiol. 5(3):293–298 (2013)

    PubMed  PubMed Central  Google Scholar 

  • Park KM, Song MW, Lee JH. Production of carotenoids by β-ionone-resistant mutant of Xanthophyllomyces dendrorhous using various carbon sources. Biotechnol. Bioproc. E. 13(2):197–203 (2008)

    Article  CAS  Google Scholar 

  • Ricci-Silva ME, Vitolo M, O-Neto JA. Protein and glucose 6-phosphate dehydrogenase releasing from baker’s yeast cells disrupted by a vertical bead mill. Process Biochem. 35:831–835 (2000)

    Article  CAS  Google Scholar 

  • Shen Q, Quek SY. Microencapsulation of astaxanthin with blends of milk protein and fiber by spray drying. J. Food Eng. 123:165–171 (2014)

    Article  CAS  Google Scholar 

  • Sila A, Ghlissi Z, Kamoun Z, Makni M, Nasri M. Astaxanthin from shrimp by-products ameliorates nephropathy in diabetic rats. Eur. J. Nutr. 54:301–307 (2015)

    Article  CAS  Google Scholar 

  • Varankovich N, Martinez MF, Nickerson MT, Korber DR. Survival of probiotics in pea protein-alginate microcapsules with or without chitosan coating during storage and in a simulated gastrointestinal environment. Food Sci. Biotechnol. 26(1):189–194 (2017)

    Article  CAS  Google Scholar 

  • Villalobos-Castillejos F, Cerezal-Mezquita P, Hernández-De Jesús ML, Barragán-Huerta BE. Production and stability of water-dispersible astaxanthin oleoresin from Phaffia rhodozyma. Int. J. Food Sci. Tech. 48(6):1243–1251 (2013)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This article was financially supported by the State Key Laboratory of Pulp and Paper Engineering [Grant No. 2017TS06], National Natural Science Foundation of China [Grant No. 51478190], and Guangzhou Science and Technology Program [Grant No. 2014 Y2 -00515].

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Correspondence to Ming-Jun Zhu.

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Chen, L., Wang, JL., Ni, H. et al. Disruption of Phaffia rhodozyma cells and preparation of microencapsulated astaxanthin with high water solubility. Food Sci Biotechnol 28, 111–120 (2019). https://doi.org/10.1007/s10068-018-0443-9

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