Release of N-nitrosamines and N-nitrosatable substances from baby bottle teats and rubber kitchen tools in Korea
Ensuring the safety of baby bottle teats and kitchen tools made from rubber is critical. Therefore, the migration of N-nitrosamines and N-nitrosatable substances from 30 teats and 45 kitchen tools to artificial saliva was analyzed by liquid chromatography–tandem mass spectrometry. The method was validated by assessing the limits of detection (0.46–3.87 μg/kg), limits of quantification (1.38–11.73 μg/kg), and recoveries (86.3–108.6%) of seven compounds. Nitrosodimethylamine (NDMA), N-nitrosopiperidine, and N-nitrosomorpholine (NMOR) migrated from baby bottle teats at concentrations of not detected (ND) to 3.67 μg/kg. NDMA and NMOR concentrations ranged from ND to 1.72 μg/kg after migration from 45 rubber kitchen tools. N-nitrosatable substances ranged from ND to 42.16 μg/kg after migration from baby bottle teats but did not migrate from rubber kitchen tools. All tested products were considered safe for use, as N-nitrosamine and N-nitrosatable substance levels did not exceed the permitted management specifications.
KeywordsN-nitrosamines N-nitrosatable substances Baby bottle teat Rubber kitchen tool LC–MS/MS
This research was supported by a Grant from the Korea Ministry of Food and Drug Safety (14161MFDS015).
Compliance with ethical standards
Conflict of interest
No potential conflicts of interest are reported by the authors.
- 2.IARC. List of Classifications. International Agency Research on Cancer, Lyon, France (2015). Available from: http://monographs.iarc.fr/ENG/Classification/latest_classif.php.
- 3.CEN. EN 12868: Child use and care articles–methods for determining the release of N-nitrosamines and N-nitrosatable Substances from elastomer or rubber teats and soothers. European Committee for Standardization (1999).Google Scholar
- 5.EU. Commission Directive 93/11/EEC. Concerning the release of the N-nitrosamines and N-nitrosatable substances from elastomer or rubber teats and soothers. Official Journal of European Union L93/37 (1993). Available from: http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:31993L0011&from=en.
- 6.ASTM. Standard specification for volatile N-nitrosamine levels in rubber nipples on pacifiers. F1313-90. American Society for Testing and Materials (2011). Available from: http://filesinstrument.com.cn/bbs/upfile/2007113010288.pdf; https://doi.org/10.1520/f1313-90r99.
- 7.European Committee for Standardization. Child use and care articles. Methods for determining the release of N-nitrosamines and N-nitrosatable substances from elastomer or rubber teats and soothers. EN 12868 (1999).Google Scholar
- 9.Nrishinha PS, Santosh C, Kushwaha Stephen WS, Steven GC. Nitrosamines in baby bottle nipples and pacifiers: occurrence, migration, and effect of infant formulas and fruit juices on in vitro formation of nitrosamines under simulated gastric conditions. J. Agric. Food Chem. 33: 428–433 (1985).CrossRefGoogle Scholar
- 15.Sen NP, Kushwaha SC, Seaman SW, Clarkson SG. Nitrosamines in baby bottle nipples and pacifiers: Occurrence, migration, and effect of infant formulas and fruit juices on in vitro formation of nitrosamines under simulated gastric conditions. J Agric. Food Chem. 33: 428–433 (1985).CrossRefGoogle Scholar
- 18.MFDS. Standards and specifications for food utensil, containers and packages. 206-210. Ministry of Food and Drug Safety (2015). Available from: https://www.mfds.go.kr/eng/eng/download.do;jsessionid=mp8xvroGqonS7pLm4YMS2aPFE3a4135esS9AVWaRCcsDaaRjglpV6IphR6ABjjIu?boardCode=17837&boardSeq=70089&fileSeq=1.
- 19.AOAC. Appendix K, Part I: Guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals. Association of Official Analytical Chemists (2013). Available from: http://www.eoma.aoac.org/app_k.pdf.