Intensifying sulforaphane formation in broccoli sprouts by using other cruciferous sprouts additions
Sulforaphane is a significant chemopreventive compound which is the predominant glucosinolate in broccoli sprouts. However, the existence of the epithiospecifier protein could direct the hydrolysis of glucosinolates toward sulforaphane nitrile formation instead of sulforaphane. Therefore, the study aimed on improving the yielding of sulforaphane in broccoli sprouts with a new method of the united hydrolysis of cruciferous sprouts. According to the results, the addition of radish, rocket and rape sprouts to broccoli sprouts could promote the hydrolysis of the glucoraphanin to anticancer effective sulforaphane to 2.03, 2.32 and 1.95-fold, respectively, compared to single broccoli sprouts. Meanwhile, the formation of non-bioactive sulforaphane nitrile in these three groups decreased greatly. However, the addition of mustard sprouts had no positive effect. These observations could make a contribution to the potential chemoprotective effects of broccoli sprouts.
KeywordsBroccoli sprouts Sulforaphane Glucoraphanin Addition Hydrolysis
High performance liquid chromatography/quadrupole-time of flight mass spectrometer
This work was supported by the fund of the Beijing Laboratory for Food Quality and Safety (Beijing Technology and Business University), the Beijing Natural Science Foundation (2162030), the Beijing Natural Science Foundation-Beijing Municipal Education Commission Joint Funding project (KZ201710020014), the National Natural Science Foundation of China (21606014), the Double First-rate Program (ylkxj03) and the 111 Project (B13005).
- 1.Ambrosone, C.B., McCann, S.E., Freudenheim, J.L., Marshall, J.R., Zhang, Y., and Shields, P.G. Breast cancer risk in premenopausal women is inversely associated with consumption of Broccoli, a source of isothiocyanates, but is not modified by GST genotype. Nutrition. 134: 1134–1138 (2004)CrossRefGoogle Scholar
- 2.Lippmann, D., Lehmann, C., Florian, S., Barknowitz, G., Haack, M., Mewis, I., Wiesner, M., Schreiner, M., Glatt, H., Brigelius-Flohé, R., and Kipp, A.P. Glucosinolates from pak choi and broccoli induce enzymes and inhibit inflammation and colon cancer differently. Food and Function 5:1073–1081 (2014)CrossRefGoogle Scholar
- 10.Hoffmann and Bremer, S. Modulation of drug metabolizing enzymes by dietary doses of sulforaphane; role in its anti-hypertensive and anti-oxidant effect in spontaneously hypertensive rats. Journal of Clinical Toxicology. 6: (2016)Google Scholar
- 14.Matusheski, N.V., Swarup, R., Juvik, J.A., Mithen, R., Bennett, M., and Jeffery, E.H. Epithiospecifier Protein from Broccoli (Brassica oleracea L. ssp. italica) Inhibits Formation of the Anticancer Agent Sulforaphane. Journal of Agriculturaland Food Chemistry. 54: 2069–2076 (2006)CrossRefGoogle Scholar
- 17.Clarke, J.D., Hsu, A., Riedl, K., Bella, D., Schwartz, S.J., Stevens, J.F., and Ho, E. Bioavailability and inter-conversion of sulforaphane and erucin in human subjects consuming broccoli sprouts or broccoli supplement in a cross-over study design. Pharmacological Research. 64: 456–463 (2011)CrossRefGoogle Scholar
- 20.Judson, H.G. and Barrell, G.K. The incidence of goitre in newborn lambs from ewes fed fodder radish, rape or Italian ryegrass with or without iodine supplementation. Proceedings of the New Zealand Society of Animal Production. 71: 66–70 (2011)Google Scholar