Antibiotic resistance, biochemical typing, and PFGE typing of Bifidobacterium strains commonly used in probiotic health foods
This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemical-type strain also had similar antibiotic resistance patterns.
KeywordsBifidobacterium Antibiotic resistance Biochemical typing Pulsed-field gel electrophoresis Probiotic health foods
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 5.Milazzo I, Speciale A, Musumeci R, Fazio D, Blandino G. Identification and antibiotic susceptibility of bacterial isolates from probiotic products available in Italy. New Microbiol. 29:281–291(2006)Google Scholar
- 7.Clinical and Laboratory Standards Institute (CLSI). Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; Approved Guideline, CLSI document M100–S20. Wayne, PA: CLSI (2010)Google Scholar
- 8.Pulse-Net protocol sections PNL04Standard Operating Procedure for Pulsenet PFGE of Listeria Monocytogenes (2008)Google Scholar
- 13.Liaskovskiĭ TM, Podgorskiĭ, VS. Assessment of probiotics according to the international organizations (FAO/WHO). Mikrobiol. Z. 67: 104–112 (2005)Google Scholar
- 18.Laplana LM., Cepero MA, Ruiz J, Zolezzi PC, Calvo MA, Erazo MC, Gómez-Lus R. Molecular typing of Staphylococcus aureus clinical isolates by pulsed-field gel electrophoresis, staphylococcal cassette chromosome mec type determination and dissemination of antibiotic resistance genes. Int. J. Antimicrob. Agents. 30:505–513 (2007)CrossRefGoogle Scholar
- 23.Poltavs’ka OA, Kovalenko NK. Antibiotic sensitivity of bifidobacteria isolated from different natural sources. Mikrobiol. Z. 67:32–39 (2005)Google Scholar
- 26.Yagüe GG, Martínez MC, Mora PB, Alonso MA, Gutiérrez MN, Martínez JA, Muñoz JL, Bellidob J, García R, Segovia MH. Molecular diversity of quinolone antibiotic resistance in genetically related clinical isolates of Staphylococcus aureus and susceptibility to newer quinolones. J. Antimicrob. Chemoth. 47:157–161 (2001)CrossRefGoogle Scholar