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Use of inductors in the control of Colletotrichum gloeosporioides and Rhizopus stolonifer isolated from soursop fruits: in vitro tests

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Abstract

Soursop (Annona muricata) is a tropical fruit that can be infected by Colletotrichum gloeosporioides and Rhizopus stolonifer. Traditional methods used for postharvest disease control include the application of fungicides, however due to their excessive use, as well as their persistence in the environment, the development of new strategies that control pathogens are required. The application of chitosan (Chi), salicylic acid (SA) and methyl jasmonate (MJ) is an environmentally-friendly alternative with antimicrobial properties and also induces defense mechanisms in plant tissues. In this study, Colletotrichum was reactivated and Rhizopus was identified using morphological features and molecular tools. In vitro, the application of 0.5 and 1.0% of Chi alone or in combination with SA and MJ decreased mycelial growth and sporulation, a complete inhibition of spore germination was obtained. Thus, the application of Chi in combination with SA and MJ could be a smart strategy to inhibit the development of pathogens that attack soursop fruit.

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Acknowledgements

The authors are grateful for the financial support from Tecnológico Nacional de México (TecNM) for Project 5214.14-P and CONACYT for the fellowship granted to Anelsy Ramos-Guerrero.

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Authors and Affiliations

  1. Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Laboratorio Integral de Investigación en Alimentos, Av. Tecnológico No. 2595, Lagos del Country, 63175, Tepic, Nayarit, Mexico

    Anelsy Ramos-Guerrero, Ramsés Ramón González-Estrada & Porfirio Gutiérrez-Martínez

  2. Instituto de Ecología, A.C. Unidad de Microscopía BioMimic, Carretera Antigua a Coatepec, No. 351, El Haya, 91070, Xalapa, Veracruz, Mexico

    Greta Hanako-Rosas

  3. Instituto Politécnico Nacional, Centro de Desarrollo de Productos Bióticos, Carretera Yautepec-Jojutla km 6. San Isidro, 62731, Yautepec, Morelos, Mexico

    Silvia Bautista-Baños

  4. Universidad de Guadalajara, Centro Universitario de la Ciénega (CUCIENEGA). Av. Universidad, No. 1115, Lindavista, 47820, Ocotlán, Jalisco, Mexico

    Gustavo Acevedo-Hernández

  5. Centro de Investigación en Alimentación y Desarrollo, Dirección de Tecnología de Alimentos de Origen Vegetal, A.C., Carretera a la Victoria km. 0.6, 83304, Hermosillo, Sonora, Mexico

    Martin Ernesto Tiznado-Hernández

Authors
  1. Anelsy Ramos-Guerrero
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  2. Ramsés Ramón González-Estrada
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  3. Greta Hanako-Rosas
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  4. Silvia Bautista-Baños
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  5. Gustavo Acevedo-Hernández
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  6. Martin Ernesto Tiznado-Hernández
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  7. Porfirio Gutiérrez-Martínez
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Correspondence to Porfirio Gutiérrez-Martínez.

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Ramos-Guerrero, A., González-Estrada, R.R., Hanako-Rosas, G. et al. Use of inductors in the control of Colletotrichum gloeosporioides and Rhizopus stolonifer isolated from soursop fruits: in vitro tests. Food Sci Biotechnol 27, 755–763 (2018). https://doi.org/10.1007/s10068-018-0305-5

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  • DOI: https://doi.org/10.1007/s10068-018-0305-5

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