Food Science and Biotechnology

, Volume 27, Issue 2, pp 499–508 | Cite as

Biodiversity and technological properties of yeasts from Turkish sourdough

  • Muhammet Arici
  • Gorkem Ozulku
  • Rusen Metin Yildirim
  • Osman Sagdic
  • M. Zeki Durak


In this study, yeasts were isolated and characterized from twelve traditional sourdough samples which belongs to Black Sea and Aegean regions of Turkey. Twenty six yeast species were isolated and identified by both 26S rDNA sequencing and FTIR spectroscopy. Saccharomyces cerevisiae (50%), Torulaspora delbrueckii (40%) and Kluyveromyces marxianus (10%) were found in 12 Turkish traditional sourdough samples. S. cerevisiae was found to be the most dominant species in Aegean region while T. delbrueckii was the most frequently isolated species in Black Sea region. Some technological properties of isolated yeast species such as acidity development, resistance to NaCI and potassium sorbate, and yeast effect on bread quality were investigated. Breads were prepared by S. cerevisiae TGM38 strain demonstrated the highest bread volume compared the other yeasts used in the study. This study showed the yeast diversity and technological properties of traditional Turkish sourdough breads fermented by chosen yeast species.


Sourdough yeast Technological properties Sourdough bread FTIR 26S rDNA 



This study was supported by Scientific Research Project Coordination Office, Yildiz Technical University (Project Number: 2012-07-05-KAP06) and General Directorate of Agricultural Research and Policies in Turkey.


  1. 1.
    Gobbetti, M. The sourdough microflora: Interactions of lactic acid bacteria and yeasts. Trends Food Sci. Technol. 7:267-274 (1998)CrossRefGoogle Scholar
  2. 2.
    Huys G, Daniel HM, De Vuyst L. Taxonomy and Biodiversity of Sourdough Yeasts and Lactic Acid Bacteria. Vol. 5, pp. 105–154. In: Handbook on Sourdough Biotechnology, Springer US, Boston, MA. (2013)Google Scholar
  3. 3.
    Birch AN, Petersen MA, Hansen AS. The aroma profile of wheat bread crumb influenced by yeast concentration and fermentation temperature. LWT - Food Sci. Technol. 50:480–488 (2013)CrossRefGoogle Scholar
  4. 4.
    Valmorri S, Tofalo R, Settanni L, Corsetti, A, Suzzi G. Yeast microbiota associated with spontaneous sourdough fermentations in the production of traditional wheat sourdough breads of the Abruzzo region (Italy). Antonie Van Leeuwenhoek. 97: 119–129 (2010)CrossRefGoogle Scholar
  5. 5.
    De Vuyst L, Harth H, Van Kerrebroeck S, Leroy F. Yeast diversity of sourdoughs and associated metabolic properties and functionalities. Int. J. Food Microbiol. 239: 26–34 (2016)CrossRefGoogle Scholar
  6. 6.
    Naumann D, Helm D, Labischinski H, Giesbrecht P. The characterization of microorganisms by Fourier-transform infrared spectroscopy (FT-IR). Pp. 43–96. In: Modern techniques for rapid microbiological analysis, VCH, New York, USA, (1991)Google Scholar
  7. 7.
    Kümmerle M, Scherer S, Seiler H, Rapid and reliable identification of food-borne yeasts by Fourier-transform infrared spectroscopy. Appl. Environ. Microbiol. 64:2207–2214 (1998)Google Scholar
  8. 8.
    Wenning M, Scherer S, Naumann D. Infrared spectroscopy in the identification of microorganisms. In: Handbook of Vibrational Spectroscopy. Wiley Online Library (2008)Google Scholar
  9. 9.
    Wenning M, Seiler H, Scherer S. Fourier-transform infrared microspectroscopy, a novel and rapid tool for identification of yeasts. Appl. Environ. Microbiol. 68:4717–4721 (2002)CrossRefGoogle Scholar
  10. 10.
    Cocolin L, Bisson LF, Mills DA. Direct profiling of the yeast dynamics in wine fermentations. FEMS Microbiol. Lett. 189:81–87 (2000)CrossRefGoogle Scholar
  11. 11.
    AACC. Approved Method of the AACC. Method 10-10. American Association of Cereal Chemists, St. Paul, MN, USA (1990)Google Scholar
  12. 12.
    AACC. Approved methods of the AACC. 10th ed. American Association of Cereal Chemists St Paul, MN. (2000)Google Scholar
  13. 13.
    Arendt EK, Ryan LAM, Dal Bello F. Impact of sourdough on the texture of bread. Food Microbiol. 24:165–174 (2007)CrossRefGoogle Scholar
  14. 14.
    Lhomme E, Lattanzi A, Dousset X, Minervini F, De Angelis M, Lacaze G, Onno B, Gobbetti M. Lactic acid bacterium and yeast microbiotas of sixteen French traditional sourdoughs. Int. J. Food Microbiol. 215:161–70 (2015)CrossRefGoogle Scholar
  15. 15.
    Minervini F, Di Cagno R, Lattanzi A, De Angelis M, Antonielli L, Cardinali G, Cappelle S, Gobbetti M. Lactic acid bacterium and yeast microbiotas of 19 sourdoughs used for traditional/typical Italian breads: Interactions between ingredients and microbial species diversity. Appl. Environ. Microbiol. AEM-07721 (2012)Google Scholar
  16. 16.
    Collar Esteve C, Benedito de Barber C, Martínez-Anaya M. Microbial sour doughs influence acidification properties and breadmaking potential of wheat dough. Jou Food Sci. 59:629–633 (1994)CrossRefGoogle Scholar
  17. 17.
    Thiele C, Gänzle MG, Vogel RV. Contribution of Sourdough Lactobacilli, Yeast, and Cereal Enzymes to the Generation of Amino Acids in Dough Relevant for Bread Flavor. Cereal Chem. 79:45–51 (2002)CrossRefGoogle Scholar
  18. 18.
    Wehrle K, Arendt EK, Rheological changes in wheat sourdough during controlled and spontaneous fermentation. Cereal Chem. 75:882–886 (1998)CrossRefGoogle Scholar
  19. 19.
    Zhang J, Liu W, Sun Z, Bao Q, Wang F, Yu J, Chen W, Zhang H. Diversity of lactic acid bacteria and yeasts in traditional sourdoughs collected from western region in Inner Mongolia of China. Food Control. 22:767–774 (2011)CrossRefGoogle Scholar
  20. 20.
    Hammes WP, Gänzle MG. Sourdough breads and related products. In Microbiology of Fermented Foods. Springer US. pp. 199–216 (1998)Google Scholar
  21. 21.
    Wenning M, Scherer S. Identification of microorganisms by FTIR spectroscopy: Perspectives and limitations of the method. Appl. Microbiol. Biotechnol. 16: 7111–7120 (2013)CrossRefGoogle Scholar
  22. 22.
    Naumann D. Infrared Spectroscopy in Microbiology. Encycl. Anal. Chem. 102–131 (2000)Google Scholar
  23. 23.
    Udelhoven T, Naumann D, Schmitt J. Development of a hierarchical classification system with artificial neural networks and FT-IR spectra for the identification of bacteria. Appl. Spectrosc. 54:1471–1479 (2000)CrossRefGoogle Scholar
  24. 24.
    Taha M, Hassan M, Essa S, Tartor Y. Use of Fourier transform infrared spectroscopy (FTIR) spectroscopy for rapid and accurate identification of yeasts isolated from human and animals. Int. J. Vet. Sci. Med. 1:15–20 (2013)CrossRefGoogle Scholar
  25. 25.
    Plessas SA, Bekatorou J, Gallanagh P, Nigam A, Koutinas A, Psarianos C. Evolution of aroma volatiles during storage of sourdough breads made by mixed cultures of Kluyveromyces marxianus and Lactobacillus delbrueckii ssp. bulgaricus or Lactobacillus helveticus. Food Chem. 107:883–889 (2008)CrossRefGoogle Scholar
  26. 26.
    Perricone M, Bevilacqua A, Corbo MR, Sinigaglia M. Technological characterization and probiotic traits of yeasts isolated from Altamura sourdough to select promising microorganisms as functional starter cultures for cereal-based products. Food Microbiol. 38:26–35 (2014)CrossRefGoogle Scholar
  27. 27.
    Manini F, Casiraghi MC, Poutanen K, Brasca M, Erba D, Plumed-Ferrer C. Characterization of lactic acid bacteria isolated from wheat bran sourdough. LWT - Food Sci. Technol. 66:275–83 (2016)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Muhammet Arici
    • 1
  • Gorkem Ozulku
    • 1
  • Rusen Metin Yildirim
    • 1
  • Osman Sagdic
    • 1
  • M. Zeki Durak
    • 1
  1. 1.Food Engineering DepartmentYildiz Technical UniversityIstanbulTurkey

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