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Food Science and Biotechnology

, Volume 27, Issue 2, pp 461–466 | Cite as

New route of chitosan extraction from blue crabs and shrimp shells as flocculants on soybean solutes

  • Yongjae Lee
  • Hyun-Wook Kim
  • Yuan H. Brad Kim
Article
  • 106 Downloads

Abstract

The chitosan extracted from blue crabs and shrimp shells using calcium oxide (deproteinization) followed by deacetylation which eliminated the demineralization step to reduce the chemical usage and environmental protection. The extracted chitosan examined the flocculation to soybean solutes. The optical density (OD), solid%, and purity% (carbohydrates/soluble solids) after flocculation were measured. The OD was significantly decreased from 0.76 to 0.16 with blue crabs and 0.06 with shrimp shells chitosan-acetate dosing (0.5 g/L). The removal of about 68 and 66% solids was achieved by the addition of 0.5 g/L chitosan-acetate. The purity% was reached about 80% with blue crabs, and 78% with shrimp shells chitosan-acetate. The results of this study verified that the calcium oxide treatment should remove protein and increase the chitin extraction yield on blue crab and shrimp shells. This new route of chitosan extraction should be a useful method for making flocculants in the soybean solutes.

Keywords

Chitosan Flocculants Calcium oxide Blue crabs Shrimp shell 

Notes

Acknowledgements

The authors wish to thank Process Engineering R&D Center staff members at Texas A&M University for equipment and technical support for this work.

References

  1. 1.
    AOAC. Official methods of analysis of the AOAC, 15th ed. Association of official analytical chemists. Arlington, VA, USA (1990)Google Scholar
  2. 2.
    Arbia W, Arbia L, Adour L, Amrane A. Chitin extraction from crustacean shells using biological methods—a review. Food Technol. Biotechnol. 51(1):12–25 (2013)Google Scholar
  3. 3.
    Bolto B, Gregory J. Organic polyelectrolytes in water treatment. Water Res. 41:2301–2324 (2007)CrossRefGoogle Scholar
  4. 4.
    Bratby J. Coagulation and flocculation in water and wastewater treatment. 2nd ed. IWA Publishing (2007)Google Scholar
  5. 5.
    Burrows F, Louine C, Abazinge M, Onokpise O. Extraction and evaluation of chitosan from crab exoskeleton as a seed fungicide and plant growth enhancer. American-Eurasia J. Agric. Environ. Sci. 2(2):103–111 (2007)Google Scholar
  6. 6.
    Chang VS, Nagwani M, Kim CH, Holtzapple MT. Oxidative lime pretreatment of high –lignin biomass: poplar wood and newspaper. Appl. Biochem. Biotechnol. 94:1–28 (2001)CrossRefGoogle Scholar
  7. 7.
    Chebotok EN, Novikov VY, Konovalova IN. Depolymerization of chitin and chitosan in the course of base deacetylation. Russ. J. Appl. Chem. 79(7):1162–1166 (2006)CrossRefGoogle Scholar
  8. 8.
    Domard A. A perspective on thirty years research on chitin and chitosan. Carbohyd. Polym. 84(2):696–703 (2011)CrossRefGoogle Scholar
  9. 9.
    Dong C, Chen W, Liu C. Flocculation of algal cells by amphoteric chitosan-based flocculant. Bioresour. Technol. 170:239–247 (2014)CrossRefGoogle Scholar
  10. 10.
    Gao Y, Cranston R. Recent advances in antimicrobial treatments of textiles. Text. Res. J. 78:62–72 (2008)Google Scholar
  11. 11.
    Giri Dev VR, Neelakandan R, Sudha S, Shamugasundram OL, Nadaraj RN. Chitosan-a polymer with wider applications. Text Mag. 46:83–86 (2005)Google Scholar
  12. 12.
    Guibal E. Interactions of metal ions with chitosan-based sorbents: a review. Sep. Purif. Technol. 38:43–74 (2004)CrossRefGoogle Scholar
  13. 13.
    Harish Prashanth KV, Tharanathan RN. Chitin/chitosan: modifications and their unlimited application- an overview. Trends Food Sci. Technol. 18:117–131 (2007)CrossRefGoogle Scholar
  14. 14.
    Havlin JL, Soltanpour PN. A nitric acid plant tissue digest method for use with inductively couple plasma spectrometry. Commun. Soil Sci. Plant Anal. 11:969–980 (1980)CrossRefGoogle Scholar
  15. 15.
    Hirohara H, Takehara M, Fukita A, Kansai KKK, Hirohara H. Flocculant and sludge treatment method. Japanese Patent JP2001129310 (1999)Google Scholar
  16. 16.
    Kandra P, Challa MM, Jyothi HKP. Efficient use of shrimp waste: present and future trends. Appl. Microbiol. Biotechnol. 93:17–29 (2012)CrossRefGoogle Scholar
  17. 17.
    Kashyap N, Kumar N, Ravi Kumar MNV. Hydrogels for pharmaceutical and biomedical applications. Crit. Rev. Ther. Drug Carrier Syst. 22:104–150 (2005)CrossRefGoogle Scholar
  18. 18.
    Khanafari A, Marandi R, Sanatei S. Recovery of chitin and chitosan from shrimp waste by chemical and microbial methods. Iran J. Environ. Health Sci. Eng. 5(1):19–24 (2008)Google Scholar
  19. 19.
    Krajewska B. Membrane-based processes performed with use of chitin/chitosan materials. Sep. Purif. Technol. 41:305–312 (2005)CrossRefGoogle Scholar
  20. 20.
    Kurita K. Chitin and chitosan: functional biopolymers from marine crustaceans. Mar. Biotechnol. 8:203–206 (2006)CrossRefGoogle Scholar
  21. 21.
    Lamarque G, Cretenet M, Viton C, Domard A. New route of deacetylation of α- and β-chitins by means of free-pump out-thaw cycles. Biomacromolecules. 6:1380–1388 (2005)CrossRefGoogle Scholar
  22. 22.
    Lim S, Hudson SM. Review of chitosan and its derivatives as antimicrobial agents and their uses as textile chemicals. J. Macromol. Sci., Reviews. 43(2):223–269 (2003)Google Scholar
  23. 23.
    Mohn F. Harvesting of micro-algal biomass. In: Borowitzka LJ, Borowitzka MA (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge. (1988)Google Scholar
  24. 24.
    Muzzarelli RAA, Rochetti R. Determination of the degree of deacetylation of chitosan by first derivative ultraviolet spectrophotometry. J. Carbohydr. Polym. 5:461–472 (1985)CrossRefGoogle Scholar
  25. 25.
    No HK, Meyers SP. Application of chitosan for treatment of wastewaters. Rev. Environ. Contam. Toxicol. 163:1–28 (2000)Google Scholar
  26. 26.
    Percot A, Viton C, Domard A. Optimization of chitin extraction from shrimp shells. Biomacromolecules. 4:12–18 (2003)CrossRefGoogle Scholar
  27. 27.
    Renault F, Sancey B, Badot PM, Crini G. Chitosan for coagulation/flocculation processes—an eco-friendly approach. Eur. Polym. J. 45:1337–1348 (2009)CrossRefGoogle Scholar
  28. 28.
    Rinaudo M. Chitin and chitosan: properties and applications. Prog. Polym. Sci. 31:603–632 (2006)CrossRefGoogle Scholar
  29. 29.
    Teli MD, Sheikh J. Extraction of chitosan from shrimp shells waste and application in antibacterial finishing of bamboo rayon. Int. J. Biol. Macromol. 50:1195–1200 (2012)CrossRefGoogle Scholar
  30. 30.
    Varma AJ, Deshpande SV, Kennedy JF. Metal complexation by chitosan and its derivatives: a review. Carbohydr. Polym. 55:77–93 (2004)CrossRefGoogle Scholar
  31. 31.
    Xu J, Cheng JJ. Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime. Bioresour. Technol. 102:3861–3868 (2011)CrossRefGoogle Scholar
  32. 32.
    Yang JK, Shih IL, Tzeng YM, Wang SL. Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes. Enzyme Microbiol. Technol. 26:406–413 (2000)CrossRefGoogle Scholar
  33. 33.
    Zeng DF, Jun L, Huan Y. Mechanism study on flocculating organic pollutants by chitosan with different molecular in wastewater. AJER. 2(5):91–95 (2013)CrossRefGoogle Scholar
  34. 34.
    Zeng D, Wu J, Kennedy JF. Application of a chitosan flocculant to water treatment. Carbohydr. Polym. 71:135–139 (2008)CrossRefGoogle Scholar
  35. 35.
    Zhang X. Complex compound chemistry. Beijing: Chinese Chemical Industry Publishers, Chap. 5 (1979)Google Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Nutrition and Food ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Department of Animal Science and BiotechnologyGyeongnam National University of Science and TechnologyJinjuSouth Korea
  3. 3.Meat Science and Muscle Biology Laboratory, Department of Animal SciencesPurdue UniversityWest LafayetteUSA

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