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Food Science and Biotechnology

, Volume 27, Issue 2, pp 575–579 | Cite as

Aged black garlic extract regulates lipid metabolism by inhibiting lipogenesis and promoting lipolysis in mature 3T3-L1 adipocytes

  • Hajin Nam
  • Harry Jung
  • Yooyeon Kim
  • Boyoung Kim
  • Kyeong Hee Kim
  • Sang Jae Park
  • Jun Gyo Suh
Article
  • 105 Downloads

Abstract

As the mechanism of aged black garlic (ABG) extract affecting lipid metabolism in adipocytes remains unclear, this study evaluated the effect of ABG extract on lipid metabolism and the expression of related proteins in mature 3T3-L1 adipocytes. ABG extract treatments at 0, 0.625, 1.25, and 2.5, and 5 mg/mL had no effect on cell morphology or viability in adipocytes. ABG extract suppressed lipogenesis and induced lipolysis in a dose-dependent manner compared to control. Furthermore, ABG extract at 2.5 and 5 mg/mL significantly reduced protein expression of proliferator activated receptor γ (PPARγ) and perilipin in mature 3T3-L1 adipocytes. The hormone-sensitive lipase (HSL) and Ser563-pHSL levels were also significantly reduced by treatment with 5 mg/mL of ABG extract. Taken together, these results suggest that ABG extract has anti-lipogenic and lipolytic effects in mature 3T3-L1 adipocytes, indicating a potential in anti-obesity therapies.

Keywords

Lipolysis Lipogenesis Mature 3T3-L1 adipocyte Aged black garlic 

Notes

Acknowledgments

We are grateful to Dr. Kyung-min Sohn for useful discussions.

Compliance with ethical standards

Conflict of interest

No potential conflict of interest was reported by the authors.

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Medical Genetics, College of MedicineHallym UniversityChuncheonKorea
  2. 2.RND TeamMedience Co. LtdChuncheonKorea

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