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Separation and identification of bromelain-generated antibacterial peptides from Actinopyga lecanora

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Abstract

Actinopyga lecanora, as a rich protein source was hydrolysed to generate antibacterial bioactive peptides using different proteolytic enzymes. Bromelain hydrolysate, after 1 h hydrolysis, exhibited the highestantibacterial activities against Pseudomonas aeruginosa, Pseudomonas sp., Escherichia coli and Staphylococcus aureus. Two dimensional fractionation strategies, using a semi-preparative RP-HPLC and an isoelectric-focusing electrophoresis, were applied for peptide profiling. Furthermore, UPLC-QTOF-MS was used for peptides identification; 12 peptide sequences were successfully identified. The antibacterial activity of purified peptides from A. lecanora on P. aeruginosa, Pseudomonas sp., E. coli and S. aureus was investigated. These identified peptides exhibited growth inhibition against P. aeruginosa, Pseudomonas sp., E. coli and S. aureus with values ranging from 18.80 to 75.30%. These results revealed that the A. lecanora would be used as an economical protein source for the production of high value antibacterial bioactive peptides.

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Acknowledgments

This work was funded by the Malaysia Ministry of Science, Technology and Innovation (MOSTI) under Project No. 10-05ABI-FB 037.

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Authors and Affiliations

  1. Acurate Food Labratory, Tajzieh Kimia Novin Azma, Tehran, Iran

    Raheleh Ghanbari

  2. Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Afshin Ebrahimpour

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  1. Raheleh Ghanbari
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  2. Afshin Ebrahimpour
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Correspondence to Raheleh Ghanbari.

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Ghanbari, R., Ebrahimpour, A. Separation and identification of bromelain-generated antibacterial peptides from Actinopyga lecanora . Food Sci Biotechnol 27, 591–598 (2018). https://doi.org/10.1007/s10068-017-0267-z

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  • DOI: https://doi.org/10.1007/s10068-017-0267-z

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