Skip to main content
Log in

Ultrasound-assisted extraction and antioxidant activity of phenolic and flavonoid compounds and ascorbic acid from rugosa rose (Rosa rugosa Thunb.) fruit

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

The present study aimed to extract total phenolic compounds (TPC), total flavonoid compounds (TFC), and ascorbic acid (AA) from the fruit of rugosa rose (Rosa rugosa Thunb.) by ultrasound-assisted extraction (UAE), and to evaluate their antioxidant activities. UAE significantly increased the extract yield compared with that obtained using the conventional method. TPC, TFC, and AA were extracted, depending on the extraction conditions (temperature, time, and ethanol concentration), in the range of 50.73–96.69, 15.93–31.88, and 3.06–6.08 mg/g, respectively. TPC and TFC were effectively extracted at a relatively high temperature (50 °C) than AA was (30 °C). The solvent condition used to extract TPC, TFC, and AA was 50% ethanol. The UAE condition for the highest antioxidant activity was obtained 30 °C, 30 min, and 50% ethanol, which were the same condition for the highest AA extraction. Among the extracts, AA showed a strong correlation with antioxidant activity at p-value of 0.001.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Jang HR, Park BJ, Park SA, Pee OJ, Park SY, Park KY. Optimization of shoot cultures and bioactive compound accumulation in Rosa rugosa during acclimatization. J. plant Biotechnol. 43: 104–109 (2016)

    Article  Google Scholar 

  2. Demir N, Yildiz O, Alpaslan M, Hayaloglu AA. Evaluation of volatiles, phenolic compounds and antioxidant activities of rose hip (Rosa L.) fruits in Turkey. LWT - Food Sci. Technol. 57: 126–133 (2014)

    Article  CAS  Google Scholar 

  3. Hashidoko Y. The phytochemistry of Rosa rugose. Phytochemistry 43: 535–549 (1996)

    Article  CAS  Google Scholar 

  4. Razungles A, Oszmianski J, Sapis JC. Determination of Carotenoids in Fruits of Rosa sp. (Rosa Canina and Rosa Rugosa) and of Chokeberry (Aronia Melanocarpa). J. Food Sci. 54: 774–775 (1989)

    Article  CAS  Google Scholar 

  5. Czyzowska A, Klewicka E, Pogorzelski E, Nowak A. Polyphenols, vitamin C and antioxidant activity in wines from Rosa canina L. and Rosa rugosa Thunb. J. Food Comp. Anal. 39: 62–68 (2015)

    Article  CAS  Google Scholar 

  6. Cabiscol E, Tamarit J, Ros J. Oxidative stress in bacteria and protein damage by reactive oxygen species. Int. Microbiol. 3: 3–8 (2010)

    Google Scholar 

  7. Kang HS, Chung HY, Kim JY, Son BW, Jung HA, Choi JS. Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch. Pharm. Res. 27: 194–198 (2004)

    Article  CAS  Google Scholar 

  8. Hayes JD, Chanas SA, Henderson CJ, McMahon M, Sun C, Moffat GJ, Yamamoto M. The Nrf2 transcription factor contributes both to the basal expression of glutathione S-transferases in mouse liver and to their induction by the chemopreventive synthetic antioxidants, butylated hydroxyanisole and ethoxyquin. Biochem. Soc. Trans. 28: 33–41 (2000)

    Article  CAS  Google Scholar 

  9. Vilkhu K, Mawson R, Simon L, Bates D. Applications and opportunities for ultrasound assisted extraction in the food industry - A review. Innov. Food Sci. Emerg. Technol. 9: 161–169 (2008)

    Article  CAS  Google Scholar 

  10. Saleh IA, Vinatoru M, Mason TJ, Abdel-Azim NS, Aboutabl EA, Hammouda FM. A possible general mechanism for ultrasound-assisted extraction (UAE) suggested from the results of UAE of chlorogenic acid from Cynara scolymus L. (artichoke) leaves. Ultrason. Sonochem. 31: 330–336 (2016)

    Article  CAS  Google Scholar 

  11. Scalbert A, Monties B, Janin G. Tannins in wood: comparison of different estimation methods. J. Agric. Food Chem. 37: 1324–1329 (1989)

    Article  CAS  Google Scholar 

  12. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555–559 (1999)

    Article  CAS  Google Scholar 

  13. Nováková L, Solich P, Solichová D. HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids. Trac-Trends Anal. Chem. 27: 942–958 (2008)

    Article  Google Scholar 

  14. Brand-Williams W, Cuvelier ME, Berset CLWT. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Technol. 28: 25–30 (1995)

    Article  CAS  Google Scholar 

  15. Re R, Pellegrini N, Proteggente A, Pannanla A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231–1237 (1999)

    Article  CAS  Google Scholar 

  16. Ferreira IC, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem. 100: 1511–1516 (2007)

    Article  CAS  Google Scholar 

  17. Ma YQ, Chen JC, Liu DH, Ye XQ. Simultaneous extraction of phenolic compounds of citrus peel extracts: effect of ultrasound. Ultrason. Sonochem. 16: 57–62 (2009)

    Article  CAS  Google Scholar 

  18. Carrera C, Ruiz-Rodríguez A, Palma M, Barroso CG. Ultrasound assisted extraction of phenolic compounds from grapes. Anal. Chim. Acta. 732: 100–104 (2012)

    Article  CAS  Google Scholar 

  19. Suslick KS, Hammerton DA, Cline RE. Sonochemical hot spot. J. Am. Chem. Soc. 108: 5641–5642 (1986)

    Article  CAS  Google Scholar 

  20. Davey MW, Montagu MV, Inzé D, Sanmartin M. Kanellis A, Smirnoff N, Benzie IJJ. Strain JJ, Favell D, Fletcher J. Plant L‐ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. J. Sci. Food Agric. 80: 825–860 (2000)

    Article  CAS  Google Scholar 

  21. Portenlänger G, Heusinger H. Chemical reactions induced by ultrasound and γ-rays in aqueous solutions of L-ascorbic acid. Carbohydr. Res. 232: 291–301 (1992)

    Article  Google Scholar 

  22. Tiwari BK, OʼDonnell CP, Patras A, Cullen PJ. Anthocyanin and ascorbic acid degradation in sonicated strawberry juice. J. Agri. Food Chem. 56: 10071–10077 (2008)

    Article  CAS  Google Scholar 

  23. Cheng VJ, Bekhit AEDA, McConnell M, Mros S, Zhao J. Effect of extraction solvent, waste fraction and grape variety on the antimicrobial and antioxidant activities of extracts from wine residue from cool climate. Food Chem. 134: 474–482 (2012)

    Article  CAS  Google Scholar 

  24. Wang J, Sun B, Cao Y, Tian Y, Li X. Optimization of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chem. 106: 804–810 (2008)

    Article  CAS  Google Scholar 

  25. Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chem. 130: 1036–1043 (2012)

    Article  CAS  Google Scholar 

  26. Ramarathnam N, Ochi H, Takeuchi M. Antioxidative defense system in vegetable extracts Natural antioxidants. pp. 76–87. In: Natural Antioxidants: Chemistry, Health Effects, and Applications. Fereidoon S (ed). AOCS press, Champaign, Illinois, USA (1997)

  27. Pinelo M, Arnous A, Meyer AS. Upgrading of grape skins: Significance of plant cell-wall structural components and extraction techniques for phenol release. Trends Food Sci. Technol. 17: 579–590 (2006)

    Article  CAS  Google Scholar 

  28. Xu BJ, Chang SKC. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci. 72: 159–166 (2007)

    Article  Google Scholar 

  29. Field M, Munafò MR, Franken IH. A meta-analytic investigation of the relationship between attentional bias and subjective craving in substance abuse. Psychol. Bull. 135: 589–607 (2009)

    Article  Google Scholar 

Download references

Acknowledgements

This research was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0020141).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jae-Won Lee.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Um, M., Han, TH. & Lee, JW. Ultrasound-assisted extraction and antioxidant activity of phenolic and flavonoid compounds and ascorbic acid from rugosa rose (Rosa rugosa Thunb.) fruit. Food Sci Biotechnol 27, 375–382 (2018). https://doi.org/10.1007/s10068-017-0247-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-017-0247-3

Keywords

Navigation