Food Science and Biotechnology

, Volume 27, Issue 2, pp 525–535 | Cite as

Development of a novel fermented pumpkin-based beverage inoculated with water kefir grains: a response surface methodology approach

  • Wee Yin Koh
  • Uthumporn Utra
  • Ahmad Rosma
  • Mohd. Esah Effarizah
  • Wan Ishak Wan Rosli
  • Yong-Ha Park


Pumpkin (Cucurbita pepo) is well known for its health and nutritional benefits and is recommended for daily consumption. This is the first report on optimization and development of fermented pumpkin-based water kefir beverage. Optimum pumpkin puree and brown sugar concentrations were found at 22.28 and 9.07% w/v, respectively, were made into a pumpkin-based beverage and fermented with water kefir grains for 24 h at 32 °C. The optimized fermented pumpkin-based water kefir beverage was found to be non-alcoholic, achieved good overall acceptability and high Lactobacillus, acetic acid bacteria and yeast cell viability of approximately 1012, 109 and 109 CFU mL−1, respectively. Overall, the optimized product attained superb technological characteristics and has the potential for industrial exploitation as a refreshing water kefir drink.


Box–Behnken design Cucurbita pepo Lactobacillus Water kefir Yeast 



The authors are grateful to the ASEAN University Network (AUN) and Korea Association of the Southeast Asian Studies for providing financial support under ASEAN-ROK Academic Exchange Programme 2016/2017; Research Universiti Grant (1001/PTEKIND/811339) and Universiti Sains Malaysia Vice-Chancellor Award Scholarship.


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Wee Yin Koh
    • 1
  • Uthumporn Utra
    • 1
  • Ahmad Rosma
    • 2
  • Mohd. Esah Effarizah
    • 1
  • Wan Ishak Wan Rosli
    • 3
  • Yong-Ha Park
    • 4
  1. 1.Food Technology Division, School of Industrial TechnologyUniversiti Sains MalaysiaMindenMalaysia
  2. 2.Bioprocess Technology Division, School of Industrial TechnologyUniversiti Sains MalaysiaMindenMalaysia
  3. 3.School of Health Sciences, Health Sciences CampusUniversiti Sains MalaysiaKubang KerianMalaysia
  4. 4.Department of Applied Microbiology and BiotechnologyYeungnam UniversityGyeongsanSouth Korea

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