Food Science and Biotechnology

, Volume 26, Issue 1, pp 245–253 | Cite as

Glycine max Merr. leaf extract possesses anti-oxidant properties, decreases inflammatory mediator production in murine macrophages, and inhibits growth, migration, and adhesion in human cancer cells

  • Youngeun Kwak
  • Jihyeung Ju


The present study aimed to investigate the in vitro anti-oxidant, anti-inflammatory, and anticancer properties of the ethanol extract of soybean (Glycine max Merr.) leaves (SLE). The total polyphenol and flavonoid levels were 142.0±14.0mg gallic acid equivalent/g and 104.9±2.0 mg quercetin equivalent/g, respectively. The radical scavenging activity and ferric-reducing anti-oxidant power of SLE at the concentrations of 125–500 μg/mL were 5–61%. In lipopolysaccharide-treated RAW 264.7 macrophages, treatment with SLE at concentrations of 62.5–500 μg/mL dose-dependently decreased the production of nitric oxide and prostaglandin E2. In both HCT116 human colon cancer cells and H1299 human lung cancer cells, treatment with SLE inhibited the growth and anchorage-independent colony formation. SLE was also effective in inhibiting the migration of H1299 cells and the adhesion of both HCT116 and H1299 cells. These results suggest that SLE exerts anti-oxidant, antiinflammatory, and anti-cancer activities in vitro. It needs to be determined whether similar effects are reproduced in vivo.


soybean leaf anti-oxidant activity anti-inflammatory activity anti-cancer activity in vitro 


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  1. 1.
    Xiao CW. Health effects of soy protein and isoflavones in humans. J. Nutr. 138: 1244S–1249S (2008)Google Scholar
  2. 2.
    Shenoy S, Bedi R, Sandhu JS. Effect of soy isolate protein and resistance exercises on muscle performance and bone health of osteopenic/osteoporotic post-menopausal women. J. Women Aging 25: 183–198 (2013)CrossRefGoogle Scholar
  3. 3.
    Setchell KD. Phytoestrogens: The biochemistry, physiology, and implications for human health of soy isoflavones. Am. J. Clin. Nutr. 68: 1333S–1346S (1998)Google Scholar
  4. 4.
    Li H, Ji HS, Kang JH, Shin DH, Park HY, Choi MS, Lee CH, Lee IK, Yun BS, Jeong TS. Soy leaf extract containing kaempferol glycosides and pheophorbides improves glucose homeostasis by enhancing pancreatic beta-Cell function and suppressing hepatic lipid accumulation in db/db mice. J. Agr. Food Chem. 63: 7198–7210 (2015)CrossRefGoogle Scholar
  5. 5.
    Ho HM, Chen RY, Leung LK, Chan FL, Huang Y, Chen ZY. Difference in flavonoid and isoflavone profile between soybean and soy leaf. Biomed. Pharmacother. 56: 289–295 (2002)CrossRefGoogle Scholar
  6. 6.
    Ho HM, Leung LK, Chan FL, Huang Y, Chen ZY. Soy leaf lowers the ratio of non-HDL to HDL cholesterol in hamsters. J. Agr. Food Chem. 51: 4554–4558 (2003)CrossRefGoogle Scholar
  7. 7.
    Ho HM, Chen R, Huang Y, Chen ZY. Vascular effects of a soy leaves (Glycine max) extract and kaempferol glycosides in isolated rat carotid arteries. Planta Med. 68: 487–491 (2002)CrossRefGoogle Scholar
  8. 8.
    Choi MS, Ryu R, Seo YR, Jeong TS, Shin DH, Park YB, Kim SR, Jung UJ. The beneficial effect of soybean (Glycine max (L.) Merr.) leaf extracts in adults with prediabetes: A randomized placebo controlled trial. Food Funct. 5: 1621–1630 (2014)CrossRefGoogle Scholar
  9. 9.
    Kim UH, Yoon JH, Li H, Kang JH, Ji HS, Park KH, Shin DH, Park HY, Jeong TS. Pterocarpan-enriched soy leaf extract ameliorates insulin sensitivity and pancreatic beta-cell proliferation in type 2 diabetic mice. Molecules 19: 18493–18510 (2014)CrossRefGoogle Scholar
  10. 10.
    Zang Y, Zhang L, Igarashi K, Yu C. The anti-obesity and anti-diabetic effects of kaempferol glycosides from unripe soybean leaves in high-fat-diet mice. Food Funct. 6: 834–841 (2015)CrossRefGoogle Scholar
  11. 11.
    Li H, Kang JH, Han JM, Cho MH, Chung YJ, Park KH, Shin DH, Park HY, Choi MS, Jeong TS. Anti-obesity effects of soy leaf via regulation of adipogenic transcription factors and fat oxidation in diet-induced obese mice and 3T3-L1 adipocytes. J. Med. Food 18: 899–908 (2015)CrossRefGoogle Scholar
  12. 12.
    Kim JE, Jeon SM, Park KH, Lee WS, Jeong TS, McGregor RA, Choi MS. Does Glycine max leaves or Garcinia cambogia promote weight-loss or lower plasma cholesterol in overweight individuals: A randomized control trial. Nutr. J. 10: 94 (2011)CrossRefGoogle Scholar
  13. 13.
    Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell B. 39: 44–84 (2007)CrossRefGoogle Scholar
  14. 14.
    Medzhitov R. Origin and physiological roles of inflammation. Nature 454: 428–435 (2008)CrossRefGoogle Scholar
  15. 15.
    Shacter E, Weitzman SA. Chronic inflammation and cancer. Oncology (Williston Park) 16: 217-226, 229; discussion 230-212 (2002)Google Scholar
  16. 16.
    Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA-Cancer J. Clin. 65: 5–29 (2015)CrossRefGoogle Scholar
  17. 17.
    Margraf T, Karnopp AR, Rosso ND, Granato D. Comparison between Folin-Ciocalteu and Prussian Blue Assays to estimate the total phenolic content of juices and teas using 96-well microplates. J. Food Sci. 80: C2397–2403 (2015)CrossRefGoogle Scholar
  18. 18.
    Csepregi K, Kocsis M, Hideg E. On the spectrophotometric determination of total phenolic and flavonoid contents. Acta Biol. Hung. 64: 500–509 (2013)CrossRefGoogle Scholar
  19. 19.
    Payet B, Shum Cheong Sing A, Smadja J. Assessment of antioxidant activity of cane brown sugars by ABTS and DPPH radical scavenging assays: Determination of their polyphenolic and volatile constituents. J. Agr. Food Chem. 53: 10074–10079 (2005)CrossRefGoogle Scholar
  20. 20.
    Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 239: 70–76 (1996)CrossRefGoogle Scholar
  21. 21.
    Ju J, Kwak Y, Hao X, Yang CS. Inhibitory effects of calcium against intestinal cancer in human colon cancer cells and Apc(Min/+) mice. Nutr. Res. Pract. 6: 396–404 (2012)CrossRefGoogle Scholar
  22. 22.
    Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal. Biochem. 126: 131–138 (1982)CrossRefGoogle Scholar
  23. 23.
    Irons R, Tsuji PA, Carlson BA, Ouyang P, Yoo MH, Xu XM, Hatfield DL, Gladyshev VN, Davis CD. Deficiency in the 15-kDa selenoprotein inhibits tumorigenicity and metastasis of colon cancer cells. Cancer Prev. Res. (Phila) 3: 630–639 (2010)CrossRefGoogle Scholar
  24. 24.
    Toton E, Ignatowicz E, Bernard MK, Kujawski J, Rybczynska M. Evaluation of apoptotic activity of new condensed pyrazole derivatives. J. Physiol. Pharmacol. 64: 115–123 (2013)Google Scholar
  25. 25.
    Kwak Y, Ju J. Inhibitory activities of Perilla frutescens britton leaf extract against the growth, migration, and adhesion of human cancer cells. Nutr. Res. Pract. 9: 11–16 (2015)CrossRefGoogle Scholar
  26. 26.
    Zhang YJ, Gan RY, Li S, Zhou Y, Li AN, Xu DP, Li HB. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules 20: 21138–21156 (2015)CrossRefGoogle Scholar
  27. 27.
    Chew YL, Chan EW, Tan PL, Lim YY, Stanslas J, Goh JK. Assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of Leguminosae medicinal plants in Peninsular Malaysia. BMC Complem. Altern. M. 11: 12 (2011)CrossRefGoogle Scholar
  28. 28.
    Tuntipopipat S, Muangnoi C, Failla ML. Anti-inflammatory activities of extracts of Thai spices and herbs with lipopolysaccharide-activated RAW 264.7 murine macrophages. J. Med. Food 12: 1213–1220 (2009)CrossRefGoogle Scholar
  29. 29.
    Moncada S, Palmer RM, Higgs EA. Nitric oxide: Physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43: 109–142 (1991)Google Scholar
  30. 30.
    Hata AN, Breyer RM. Pharmacology and signaling of prostaglandin receptors: Multiple roles in inflammation and immune modulation. Pharmacol. Therapeut. 103: 147–166 (2004)CrossRefGoogle Scholar
  31. 31.
    Hanahan D, Weinberg RA. Hallmarks of cancer: The next generation. Cell 144: 646–674 (2011)CrossRefGoogle Scholar
  32. 32.
    Ballet F, Petit J, Poupon R, Darnis F. Soft agar clonogenic assay for predicting chemosensitivity of human tumor cells from malignant effusions. Biomedicine 35: 177–178 (1981)Google Scholar
  33. 33.
    Hotz MA, Gong J, Traganos F, Darzynkiewicz Z. Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry 15: 237–244 (1994)CrossRefGoogle Scholar
  34. 34.
    Zang Y, Sato H, Igarashi K. Anti-diabetic effects of a kaempferol glycoside-rich fraction from unripe soybean (Edamame, Glycine max L. Merrill. ‘Jindai’) leaves on KK-A(y) mice. Biosci. Biotech. Bioch. 75: 1677–1684 (2011)CrossRefGoogle Scholar
  35. 35.
    Yuk HJ, Curtis-Long MJ, Ryu HW, Jang KC, Seo WD, Kim JY, Kang KY, Park KH. Pterocarpan profiles for soybean leaves at different growth stages and investigation of their glycosidase inhibitions. J. Agr. Food Chem. 59: 12683–12690 (2011)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Department of Food and NutritionChungbuk National UniversityCheongju, ChungbukKorea

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