Glycine max Merr. leaf extract possesses anti-oxidant properties, decreases inflammatory mediator production in murine macrophages, and inhibits growth, migration, and adhesion in human cancer cells
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The present study aimed to investigate the in vitro anti-oxidant, anti-inflammatory, and anticancer properties of the ethanol extract of soybean (Glycine max Merr.) leaves (SLE). The total polyphenol and flavonoid levels were 142.0±14.0mg gallic acid equivalent/g and 104.9±2.0 mg quercetin equivalent/g, respectively. The radical scavenging activity and ferric-reducing anti-oxidant power of SLE at the concentrations of 125–500 μg/mL were 5–61%. In lipopolysaccharide-treated RAW 264.7 macrophages, treatment with SLE at concentrations of 62.5–500 μg/mL dose-dependently decreased the production of nitric oxide and prostaglandin E2. In both HCT116 human colon cancer cells and H1299 human lung cancer cells, treatment with SLE inhibited the growth and anchorage-independent colony formation. SLE was also effective in inhibiting the migration of H1299 cells and the adhesion of both HCT116 and H1299 cells. These results suggest that SLE exerts anti-oxidant, antiinflammatory, and anti-cancer activities in vitro. It needs to be determined whether similar effects are reproduced in vivo.
Keywordssoybean leaf anti-oxidant activity anti-inflammatory activity anti-cancer activity in vitro
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- 1.Xiao CW. Health effects of soy protein and isoflavones in humans. J. Nutr. 138: 1244S–1249S (2008)Google Scholar
- 3.Setchell KD. Phytoestrogens: The biochemistry, physiology, and implications for human health of soy isoflavones. Am. J. Clin. Nutr. 68: 1333S–1346S (1998)Google Scholar
- 4.Li H, Ji HS, Kang JH, Shin DH, Park HY, Choi MS, Lee CH, Lee IK, Yun BS, Jeong TS. Soy leaf extract containing kaempferol glycosides and pheophorbides improves glucose homeostasis by enhancing pancreatic beta-Cell function and suppressing hepatic lipid accumulation in db/db mice. J. Agr. Food Chem. 63: 7198–7210 (2015)CrossRefGoogle Scholar
- 15.Shacter E, Weitzman SA. Chronic inflammation and cancer. Oncology (Williston Park) 16: 217-226, 229; discussion 230-212 (2002)Google Scholar
- 24.Toton E, Ignatowicz E, Bernard MK, Kujawski J, Rybczynska M. Evaluation of apoptotic activity of new condensed pyrazole derivatives. J. Physiol. Pharmacol. 64: 115–123 (2013)Google Scholar
- 29.Moncada S, Palmer RM, Higgs EA. Nitric oxide: Physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43: 109–142 (1991)Google Scholar
- 32.Ballet F, Petit J, Poupon R, Darnis F. Soft agar clonogenic assay for predicting chemosensitivity of human tumor cells from malignant effusions. Biomedicine 35: 177–178 (1981)Google Scholar