Anti-oxidative and anti-inflammatory activities of devil’s club (Oplopanax horridus) leaves
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This study aimed to investigate the anti-oxidative properties of the ethanolic extracts of the devil’s club (Oplopanax horridus) leaves, stems, and roots. Furthermore, the anti-inflammatory activity of the leaf extract was analyzed. The leaf extract had higher total phenolic and flavonoid contents and anti-oxidative activity (radical scavenging, reducing power, and inhibition of lipid oxidation) than the root and stem extracts. The leaf extract also had anti-inflammatory effects. It significantly reduced lipopolysaccharide (LPS)-induced nitric oxide (NO; 71.0% at 50 μg/mL), tumor necrosis factor (TNF)-α (87.6% at 100 μg/mL), and interleukin (IL)-6 (36.2% at 100 μg/mL) production in murine RAW 264.7 macrophages. Furthermore, LPS-induced inducible nitric oxide synthase (iNOS) expression was decreased by the leaf extract (IC50=24.4 μg/mL). The ultra performance liquid chromatography-diode array detector (UPLC-DAD) analysis showed that the leaf extract contained gallic acid, protocatechuic acid, chlorogenic acid, and maltol. These findings suggest that the leaf extract could be utilized as a functional food material because of its anti-oxidative and anti-inflammatory activities.
KeywordsOplopanax horridus anti-oxidative activity anti-inflammatory activity phenolic compounds UPLC-DAD
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- 10.Lantz TC, Swerhun K, Turner NJ. Devil's club (Oplopanax horridus): An ethnobotanical review. HerbalGram 62: 33–48 (2004)Google Scholar
- 11.Singleton VS, Rossi JA. Colorimetric of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am. J. Enol. Viticult 16: 144–158 (1965)Google Scholar
- 18.Taviano MF, Marino A, Trovato A, Bellinghieri V, Melchini A, Dugo P, Cacciola F, Donato P, Mondello L, Güvenç A, de Pasquale R, Miceli N. Juniperus oxycedrus L. subsp. oxycedrus and Juniperus oxycedrus L. subsp. macrocarpa (Sibth. & Sm.) Ball. “berries” from Turkey: Comparative evaluation of phenolic profile, antioxidant, cytotoxic and antimicrobial activities. Food Chem. Toxicol. 58: 22–29 (2013)Google Scholar
- 26.Osawa T. Novel natural antioxidants for utilization in food and biological systems. pp. 241–251. In: Postharvest Biochemistry of Plant Food-Materials in the Tropics. Uritani I, Garcia VV, Mendoza EM (eds). Japan Scientific Societies Press, Tokyo, Japan (1994)Google Scholar