Gelidium amansii ethanol extract suppresses fat accumulation by down-regulating adipogenic transcription factors in ob/ob mice model
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The purpose of this study was to determine the anti-obesity effects of Gelidium amansii extract (GAE) in the C57BL/6J-ob/ob mice. The ob/ob mice were fed GAE at 0.5% for 4 weeks, after which body weight, epididymal adipose tissue weight, plasma triglycerides, and hepatic lipid accumulation were significantly reduced in GAE-fed mice compared with ob/ob control mice. Plasma adiponectin levels were significantly higher in GAE-fed mice than in ob/ob control mice. These findings were supported by the expression levels of enzymes and proteins related to lipid metabolism assessed by western blotting: protein expression levels of the peroxisome proliferator-activated receptor γ and CCATT/enhancer binding protein α decreased significantly, while hormone-sensitive lipase and phospho-AMP-activated protein kinase levels increased in the GAE-fed mice compared with ob/ob control mice. These findings demonstrate that GAE regulates plasma lipid profiles and increasing highdensity lipoprotein cholesterol levels as well as by regulating the expression levels of lipid metabolic factors, resulting in reduced weight gain in ob/ob mice.
KeywordsGelidium amansii extract C57BL/6J ob/ob mice adipogenesis lipolysis anti-obesity
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- 1.Nieves JW, Komar L, Cosman F, Lindsay R. Calcium potentiates the effect of estrogen and calcitonin on bone mass: Review and analysis. Am. J. Clin. Nutr. 67: 18–24 (1998)Google Scholar
- 2.Kopelman PG. Obesity as a medical problem. Nature 404: 635–643 (2000)Google Scholar
- 15.Friedewald WT, Levy RI, Fredirickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifusege. Clin. Chem. 18: 499–502 (1972)Google Scholar
- 16.Folch J, Less M, sloan-Stanley GH. A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497–509 (1957)Google Scholar
- 31.Bruun JM, Lihn AS, Werdich C, Pedersen SB, Toubro S, Astrup A, Richelsen B. Regulation of adiponectin by adipose tissue-derived cytokines: In vivo and in vitro investigations in humans. Am. J. Physiol.-Endoc. M. 285: 527–533 (2003)Google Scholar
- 35.Jaworski K, Sarkadi-Nagy E, Duncan RE, Ahmadian M, Sul HS. Regulation of triglyceride metabolism. IV. Hormonal regulation of lipolysis in adipose tissue. Am. J. Physiol.-Gastr. L. 293: 1–4 (2007)Google Scholar
- 37.Jungtrakoon P, Plengvidhya N, Tangjittipokin W, Chimnaronk S, Salaemae W, Chongjaroen N, Charnprasert K, Sujjitjoon J, Srisawat C, Yenchitsomanus PT. Novel adiponection variants identified in type 2 diabetic patients reveal multimerization and secretion defects. PLoS ONE 6: 26792 (2011)CrossRefGoogle Scholar
- 40.Gregoire FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiol. Rev. 78: 783–809 (1998)Google Scholar
- 44.Mohammed A, Al-Numair KS, Balakrishana A. Docking studies on the interaction of flavonoids with fat mass and obesity associated protein. Pak. J. Pharm. Sci. 28: 1647–1653 (2015)Google Scholar