Food Science and Biotechnology

, Volume 26, Issue 1, pp 201–206 | Cite as

Antioxidant activities of honeybee products and their mixtures



In this study, the total phenolic content (TPC), antioxidant activity, and free radical scavenging activities (FRSA) of 70 samples comprising honeybee products (honey, pollen, royal jelly, and propolis) and their mixtures were determined. The TPC was determined in accordance with the Folin–Ciocalteu method, antioxidant activity with phosphomolybdenum, and FRSA with the 1,1-diphenyl-2-picryl hydrazyl (DPPH) assays. Honeybee propolis showed the greatest TPC, antioxidant activity, and FRSA followed by pollen, honey, and royal jelly, respectively. A positive correlation was observed between TPC and antioxidant activity of honey, pollen and mixed samples (respectively, r=0.91, r=0.93 and r=0.92) (p<0.01). Similarly, honey and mixed samples exhibited positive correlations with TPC and FRSA (respectively, r=0.98 and r=0.92) (p<0.01). It was concluded that honeybee products and their mixtures have antioxidant activity and FRSA and these effects may be attributed to their phenolic content.


honeybee products total phenolic content antioxidant activity free radical scavenging activity 


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  1. 1.
    Halliwell B, Gutteridge JMC. The antioxidant of human extracellular fluids. Arch. Biochem. Biophys. 280: 1–8 (1990)CrossRefGoogle Scholar
  2. 2.
    Nagai T, Sakai M, Inoue R, Inoue H, Suzuki N. Antioxidative activities of some commercially honeys, royal jelly, and propolis. Food Chem. 5: 237–240 (2001)CrossRefGoogle Scholar
  3. 3.
    Koc AN, Silici S, Kasap F, Oz H, Mavus-Buldu H, Ercal BD. Antifungal activity of the honeybee products against Candida spp. and Trichosporon spp. J. Med. Food 14: 128–134 (2011)CrossRefGoogle Scholar
  4. 4.
    Kanbur M, Eraslan G, Silici S. Antioxidant effect of propolis against exposure to propetamphos in rats. Ecotox. Environ. Safe. 72: 909–915 (2009)CrossRefGoogle Scholar
  5. 5.
    Silici S, Ekmekcioglu O, Eraslan G, Demirtas A. Antioxidative effect of royal jelly in cisplatin induced testes damage. Urology 74: 545–551 (2009)CrossRefGoogle Scholar
  6. 6.
    Bogdanov S. Nature and origin of the antibacterial substances in honey. LWTFood Sci. Technol. 30: 748–753 (1997)Google Scholar
  7. 7.
    Gheldof N, Engeseth NJ. Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J. Agr. Food. Chem. 50: 3050–3055 (2002)CrossRefGoogle Scholar
  8. 8.
    Al-Mamary M, Al-Meeri A, Al-Habori M. Antioxidant activities and total phenolics of different types of honey. Nutr. Res. 22: 1041–1047 (2002)CrossRefGoogle Scholar
  9. 9.
    Ferreres F, Garcia-Viguera C, Tomas-Lorente F, Tomas-Barberan FA. Hesperetin: A marker of the floral origin of citrus honey. J. Agr. Food. Chem. 61: 121–123 (1993)CrossRefGoogle Scholar
  10. 10.
    Haydak MH. Honey bee nutrition. Annu. Rev. Entomol. 15: 143–156 (1970)CrossRefGoogle Scholar
  11. 11.
    Marcucci MC, Ferreres F, Garcia-Viguera C, Bankova VS, de Castro SL, Dantas AP, Valente PH, Paulino N. Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol. 74: 105–112 (2001)CrossRefGoogle Scholar
  12. 12.
    Greenaway W, May J, Scaysbrook T, Whatley FR. Identification by gas chromatography-mass spectrometry of 150 compounds in propolis. Z. Naturforsch. C 46: 111–121 (1991)Google Scholar
  13. 13.
    Yonar ME, Yonar SM, Pala, A, Silici S, Saglam N. Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: Ameliorative effect of propolis. Dis. Aquat. Organ. 114: 209–2016 (2015)CrossRefGoogle Scholar
  14. 14.
    Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144–158 (1965)Google Scholar
  15. 15.
    Silici S, Sagdic O, Ekici L. Total phenolic content, antiradical, antioxidant and antimicrobial activities of Rhododendron honeys. Food Chem. 121: 238–243 (2010)CrossRefGoogle Scholar
  16. 16.
    Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formulation of a phosphomolybdenum complex: Specific application of vitamin E. Anal. Biochem. 269: 337–341 (1999)CrossRefGoogle Scholar
  17. 17.
    Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen. Pharmacol. 32: 661–667 (1999)CrossRefGoogle Scholar
  18. 18.
    Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E. Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem. Toxicol. 46: 3774–3779 (2008)CrossRefGoogle Scholar
  19. 19.
    Pichichero E, Canuti L, Canini A. Characterisation of the phenolic and flavonoid fractions and antioxidant power of Italian honeys of different botanical origin. J. Sci. Food Agr. 89: 609–616 (2009)CrossRefGoogle Scholar
  20. 20.
    Dobre I, Gadei G, Patrascu L, Elisei AM, Segal R. The antioxidant activity of selected Romanian honeys. The Annals of the University Dunarea de Jos of Galati Fascicle VI, Food Technol. 34: 67–73 (2010)Google Scholar
  21. 21.
    Bertoncelj J, Dobersek U, Jamnik M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem. 105: 822–828 (2007)CrossRefGoogle Scholar
  22. 22.
    Fatrcova-Sramkova K, Nazkova J, Kacaniova M, Mariassyova M, Rovna K, Stricik M. Antioxidant and antimicrobial properties of monofloral bee pollen. J. Environ. Sci. Heal. B 48: 133–138 (2013)CrossRefGoogle Scholar
  23. 23.
    Leja M, Mareczek A, Wyzgolik G, Klepacz-Baniak J, Czekonska K. Antioxidative properties of bee pollen in selected plant species. Food Chem. 100: 237–240 (2007)CrossRefGoogle Scholar
  24. 24.
    Kroyer G, Hegedus N. Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innov. Food Sci. Emerg. 2: 171–174 (2001)CrossRefGoogle Scholar
  25. 25.
    Morais M, Moreira L, Feas X, Estevinho LM. Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food Chem. Toxicol. 49: 1096–1101 (2011)CrossRefGoogle Scholar
  26. 26.
    Mejias E, Montenegro G. The antioxidant activity of Chilean honey and bee pollen produced in the Llaima Volcano’s zones. J. Food Quality 35: 315–322 (2012)CrossRefGoogle Scholar
  27. 27.
    Le Blanc BW, Davis OK, Boue S, De Lucca A, Deeby T. Antioxidant activity of Sonoran Desert bee pollen. Food Chem. 115: 1299–1305 (2009)CrossRefGoogle Scholar
  28. 28.
    Marghitas LA, Stanciu OG, Dezmirean DS, Popescu O, Bogdanov S, Campos M. In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem. 115: 878–883 (2009)CrossRefGoogle Scholar
  29. 29.
    Guo H, Kozuma Y, Yonekura M. Isolation and properties of antioxidative peptides fom water-soluble royal jelly protein hydrolysate. Food Sci. Technol. Res. 11: 222–230 (2005)CrossRefGoogle Scholar
  30. 30.
    Eraslan G, Kanbur M, Silici S, Altýnordulu S, Karabacak M. Effects of Cypermethrin on some biochemical changes in rats: The protective role of propolis. Exp. Anim. Tokyo 57: 453–460 (2008)CrossRefGoogle Scholar
  31. 31.
    Kumazawa S, Hamasaka T, Nakayama T. Antioxidant activity of propolis of various geographic origins. Food Chem. 84: 329–339 (2004)CrossRefGoogle Scholar
  32. 32.
    Gulcin I, Bursal E, Sehitoglu MH, Bilsel M, Goren AC. Polyphenol contents and antioxidant activity of lyophilized aqueus extract of propolis from Erzurum, Turkey. Food Chem. Toxicol. 48: 2227–2238 (2010)CrossRefGoogle Scholar
  33. 33.
    Moreira L, Dias LD, Pereira JA, Estevinho L. Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem. Toxicol. 46: 3482–3485 (2008)CrossRefGoogle Scholar
  34. 34.
    Buratti S, Bendetti S, Cosio MS. Evaluation of the antioxidant power of honey, propolis and royal jelly by amperometric flow injection analysis. Talanta 71: 1387–1392 (2007)CrossRefGoogle Scholar
  35. 35.
    Manthey JA, Grohmann K. Phenols in citrus peel byproducts. Concentrations of hydroxycinnamates and polymethoxylated flavones in citrus peel molasses. J. Agr. Food Chem. 49: 3268–3273 (2001)Google Scholar
  36. 36.
    Nagai T, Inoue R. Preparation and the functional properties of water extract and alkaline extract of royal jelly. Food Chem. 84: 181–186 (2004)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Erciyes UniversitySafiye Cikrikcioglu Vocational SchoolKayseriTurkey
  2. 2.Department of Agricultural BiotechnologyErciyes University, Agriculture FacultyKayseriTurkey

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