Food Science and Biotechnology

, Volume 26, Issue 1, pp 195–200 | Cite as

In vitro anti-obesity effects of sesamol mediated by adenosine monophosphate-activated protein kinase and mitogen-activated protein kinase signaling in 3T3-L1 cells

  • Geon Go
  • Jung-Suk Sung
  • Seung-Cheol Jee
  • Min Kim
  • Won-Hee Jang
  • Kyu-Young Kang
  • Dae-Young Kim
  • Sihyoung Lee
  • Han-Seung Shin
Article

Abstract

Sesamol is a phenol derivative of sesame oil and a potent anti-oxidant, anti-inflammatory, anti-hepatotoxic, and anti-aging compound. We investigated the effects of sesamol on the molecular mechanisms of adipogenesis in 3T3-L1 preadipocytes. The intracellular lipid accumulation accompanied by increased extracellular release of free glycerol was decreased during differentiation on treating 3T3-L1 with sesamol. Sesamol treatment on 3T3-L1 inhibited adipogenic differentiation by down-regulating adipogenesis-related factors (C/EBPα, PPARγ, and SREBP-1). Lipid accumulation was repressed by decreasing fatty acid synthase and by up-regulating lipolysis-response genes (HSL and LPL). The molecular mechanisms of sesamol-induced inhibition in adipogenesis were mediated by increased levels of phosphorylated adenosine monophosphate-activated protein kinase and its substrate acetyl-CoA carboxylase. Sesamol treatment, in turn, modulated the different members of the mitogenactivated protein kinase family by suppressing phosphorylation of ERK 1/2 and JNK and by increasing the phosphorylation of p38. In summary, sesamol inhibits adipogenic differentiation by reducing phosphorylation levels of ERK 1/2 and JNK while inducing lipolysis by activating p38 and AMPK. Our results demonstrate that the molecular mechanisms of in vitro anti-obesity effects of sesamol are due to the combined effects of preventing both lipid accumulation and adipogenesis.

Keywords

sesamol adipogenesis anti-obesity AMPK lipolysis MAPK 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Geon Go
    • 1
  • Jung-Suk Sung
    • 1
  • Seung-Cheol Jee
    • 1
  • Min Kim
    • 1
  • Won-Hee Jang
    • 1
  • Kyu-Young Kang
    • 2
  • Dae-Young Kim
    • 2
  • Sihyoung Lee
    • 3
  • Han-Seung Shin
    • 3
  1. 1.Department of Life ScienceDongguk University-SeoulGoyang, GyeonggiKorea
  2. 2.Department of Biological and Environmental ScienceDongguk University-SeoulGoyang, GyeonggiKorea
  3. 3.Department of Food Science and BiotechnologyDongguk University-SeoulGoyang, GyeonggiKorea

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