Survival of probiotics in pea protein-alginate microcapsules with or without chitosan coating during storage and in a simulated gastrointestinal environment
- 168 Downloads
Pea protein-alginate microcapsules with or without a chitosan coating and containing Lactobacillus rhamnosus R0011 and L. helveticus R0052 were produced by extrusion and tested for survivability during storage and in an in vitro gastrointestinal environment. Both microcapsule formulations provided significant protection for cells incubated in synthetic stomach juice at 37°C for 2 h, followed by 3 h in simulated intestinal fluid, relative to non-encapsulated bacteria. However, evaluation of cell viability during 9 weeks of storage at room temperature revealed that chitosan coating significantly improved microcapsule performance compared to non-coated microcapsules. Refrigerated storage had no negative impact on the microcapsule protection ability of both types of microcapsules. Notably, chitosan-containing microcapsules showed much higher bacterial survival counts during challenge tests even after storage. Moreover, the addition of chitosan to the microcapsule formulation did not increase the microcapsule size.
Keywordsencapsulation pea protein alginate probiotic chitosan coating
Unable to display preview. Download preview PDF.
- 1.FAO/WHO. Report of a Joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Food and Agriculture Organization of the United Nations, World Health Organization, Geneva, Switzerland (2001)Google Scholar
- 2.Varankovich NV, Nickerson MT, Korber DR. Probiotic-based strategies for therapeutic and prophylactic use against multiple gastrointestinal diseases. Front. Microbiol. 685: 1–14 (2015)Google Scholar
- 4.Jiménez-Pranteda ML, Aguilera M, McCartney AL, Hoyles L, Jiménez-Valera M, Náder-Macías ME, Ramos-Cormenzana A, Monteoliva-Sánchez M. Investigation of the impact of feeding Lactobacillus plantarum CRL 1815 encapsulated in microbially derived polymers on the rat fecal microbiota. J. Appl. Microbiol. 113: 399–410 (2012)CrossRefGoogle Scholar
- 13.Türkoðlu T, Taþcýoðlu S. Novel strategy for the ionotropic crosslinking of chitosan-alginate polyelectrolyte complexes. J. Appl. Polym. Sci. 131: 1–7 (2014)Google Scholar
- 26.Zanjani MAK, Tarzi BG, Sharifan A, Mohammadi N. Microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition. Iran. J. Pharm. Res. 13: 843–852 (2013)Google Scholar