Optimizing bioconversion of ferulic acid to vanillin by Bacillus subtilis in the stirred packed reactor using Box-Behnken design and desirability function
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A stirring bioreactor packed with a carbon fiber textiles (FT) biofilm formed by Bacillus subtilis was used to produce vanillin from ferulic acid. Biofilm formation was characterized by scanning electron microscopy. The interactive effects of three variables on vanillin molar yield (M) and conversion efficiency of ferulic acid (E) were evaluated by response surface methodology (RSM) with a Box-Behnken design (BBD). The optimal conversion conditions with a maximum overall desirability D of 0.983 were obtained by a desirability function. Considering the actual operation, the confirmation tests were performed using the slightly modified optimal conditions (initial ferulic acid concentration 1.55 g/L, temperature 35°C, stirring speed 220 rpm). The results showed that M and E were 57.42 and 93.53%, respectively. This was only 1.03% and 1.87%, respectively, different from the predicted values, confirming the validity of the predicted models. These revealed that the stirred packed reactor could be successfully used in vanillin bioconversion from ferulic acid.
Keywordsvanillin stirred packed reactor Bacillus subtilis desirability function response surface methodology
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- 1.Ma Xk, Daugulis AJ. Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing byproduct formation. Bioproc. Biosyst. Eng. 37: 891–899 (2014)Google Scholar
- 2.Vyrides I, Agathangelou M, Dimitriou R, Souroullas K, Salamex A, Ioannou A, Koutinas M. Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid. World J. Microb. Biot. 31: 1291–1296 (2015)Google Scholar
- 10.Torre P, de Faveri D, Perego P, Ruzzi M, Barghini P, Gandolfi R, Converti A. Bioconversion of ferulate into vanillin by Escherichia coli strain JM109/pBB1 in an immobilized-cell reactor. Ann. Microbiol. 54: 517–527 (2004)Google Scholar
- 16.Chen P, Li S, Yan L, Wang N, Yan X, Li H. Draft genome sequence of Bacillus subtilis type strain B7-S, which converts ferulic acid to vanillin. Genome Announc. 2: e00025–14 (2014)Google Scholar
- 18.Derringer G, Suich R. Simultaneous Optimization of several response variables. J. Qual. Technol. 12: 214–219 (1980)Google Scholar
- 19.Guilbaud M, Piveteau P, Desvaux M, Brisse S, Briandet R. Exploring the diversity of Listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of the honeycomblike morphotype. Appl. Environ. Microb. 81: 1813–1819 (2015)CrossRefGoogle Scholar
- 20.Daneshi A, Younesi H, Ghasempouri SM, Sharifzadeh M. Production of poly-3-hydroxybutyrate by Cupriavidus necator from corn syrup: Statistical modeling and optimization of biomass yield and volumetric productivity. J. Chem. Tech. Biot. 85: 1528–1539 (2010)Google Scholar
- 29.Sun ML, Liu SB, Qiao LP, Chen XL, Pang Xh, Shi M, Zhang XY, Qin QL, Zhou BC, Zhang YZ. A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: Low-cost fermentation, moisture retention, and antioxidant activities. Appl. Microbiol. Biot. 98: 7437–7445 (2014)CrossRefGoogle Scholar
- 37.Li X, Yang J, Li X, Gu W, Huang J, Zhang K-Q. The metabolism of ferulic acid via 4-vinylguaiacol to vanillin by Enterobacter sp. Px6-4 isolated from Vanilla root. Process Biochem. 43: 1132–1137 (2008)Google Scholar