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Food Science and Biotechnology

, Volume 26, Issue 1, pp 15–19 | Cite as

Determination of benzo[a]pyrene in camellia oil via vortex-assisted extraction using the UPLC-FLD method

  • Runhong Mo
  • Yanping Zhang
  • Zhanglin Ni
  • Fubin Tang
Article

Abstract

In this paper, vortex-assisted extraction using the ultraperformance liquid chromatography analysis method was performed to determine benzo[a]pyrene in camellia oil. Optimum results were obtained when 0.5 g of oil sample was used followed by vortex-assisted extraction for 10 min with 25 mL of acetonitrile. Chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus C18 column (2.1mm×100mm, particle size 1.8 μm). The optimum mobile phase comprised 70% acetone and 30% water. The detection limit of benzo[a]pyrene was 0.2 μg/kg. The recoveries were in the range of 81.0–97.0%. The proposed method was simple and fast, and it provided high throughput in the determination of benzo[a]pyrene in an oil matrix sample.

Keywords

benzo[a]pyrene vortex-assisted extraction UPLC camellia oil 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Runhong Mo
    • 1
  • Yanping Zhang
    • 1
  • Zhanglin Ni
    • 1
  • Fubin Tang
    • 1
  1. 1.Research Institute of Subtropical ForestryChinese Academy of ForestryFuyang, ZhejiangChina

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