Determination of benzo[a]pyrene in camellia oil via vortex-assisted extraction using the UPLC-FLD method
- 121 Downloads
In this paper, vortex-assisted extraction using the ultraperformance liquid chromatography analysis method was performed to determine benzo[a]pyrene in camellia oil. Optimum results were obtained when 0.5 g of oil sample was used followed by vortex-assisted extraction for 10 min with 25 mL of acetonitrile. Chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus C18 column (2.1mm×100mm, particle size 1.8 μm). The optimum mobile phase comprised 70% acetone and 30% water. The detection limit of benzo[a]pyrene was 0.2 μg/kg. The recoveries were in the range of 81.0–97.0%. The proposed method was simple and fast, and it provided high throughput in the determination of benzo[a]pyrene in an oil matrix sample.
Keywordsbenzo[a]pyrene vortex-assisted extraction UPLC camellia oil
Unable to display preview. Download preview PDF.
- 10.Noh S, Yoon SH. Stereospecific positional distribution of fatty acids of Camellia (Camellia japonica L.) seed oil. J. Food Sci. 77: C1055-C1057 (2012)Google Scholar
- 12.Vasquez V, Baez ME, Bravo M, Fuentes E. Determination of heavy polycyclic aromatic hydrocarbons of concern in edible oils via excitation-emission fluorescence spectroscopy on nylon membranes coupled to unfolded partial least-squares/residual bilinearization. Anal. Bioanal. Chem. 405: 7497–7507 (2013)CrossRefGoogle Scholar
- 13.Tang F, Mo R, Zhong D, Shen D, Fei X, Ni Z, Qu M. Saponification-extraction and HPLC determination of benzo[a]pyrene in oil-tea camellia seed oil. China Oil. Fat. 37: 62–64 (2012)Google Scholar
- 15.Bogusz MJ, El Hajj SA, Ehaideb Z, Hassan H, Al-Tufail M. Rapid determination of benzo[a]pyrene in olive oil samples with solid-phase extraction and lowpressure, wide-bore gas chromatography-mass spectrometry and fast liquid chromatography with fluorescence detection. J. Chromatogr. A 1026: 1–7 (2004)CrossRefGoogle Scholar
- 21.Yu Y, Jin Q, Wang XG. Rapid assay of polycyclic aromatic hydrocarbons in edible oils by HPLC with FLD detection without clean-up. J. Chem. Pharm. Res. 5: 409–414 (2013)Google Scholar
- 24.Morisaki H, Nakamura S, Tang N, Toriba A, Hayakawa K. Benzo[c]fluorene in urban air: Hplc determination and mutagenic contribution relative to benzo[a]pyrene. Anal. Sci. 32: 233–236 (2016) 25. Çorman ME, Armutcu C, Uzun L, Denizli A. Rapid, efficient and selective preconcentration of benzo[a]pyrene (BaP) by molecularly imprinted composite cartridge and HPLC. Mat. Sci. Eng. C 70: 41-53(2016)CrossRefGoogle Scholar
- 26.Commission European Communities. Commission of the European Communities-Commission regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs, Official J. Eur. Communities 2006;L364:5–24. foodstuffs (2006)Google Scholar
- 27.Luo F, Fei X, Fang X, Chen Y, Wang Y. Removing benzopyrene from oil-tea camellia seed oil by different decoloring agents in bleaching process. China Oil. Fat. 37: 47–50 (2012)Google Scholar