Clinical Rheumatology

, Volume 37, Issue 5, pp 1229–1237 | Cite as

Anti-tubulin-α-1C autoantibody in systemic lupus erythematosus: a novel indicator of disease activity and vasculitis manifestations

  • Xiaozhen Zhao
  • Yongjing Cheng
  • Yuzhou Gan
  • Rulin Jia
  • Lei Zhu
  • Xiaolin Sun
Original Article


A variety of autoantibodies has been involved in the pathogenesis of systemic lupus erythematosus (SLE), some of which are well known and applied as disease biomarkers. This study aimed to determine the prevalence of a novel autoantibody, anti-tubulin-α-1C, in patients with SLE and investigate its clinical significance. Anti-tubulin-α-1C autoantibody levels were determined by enzyme-linked immunosorbent assay (ELISA) in 128 SLE patients, 38 primary Sjögren’s syndrome (pSS) patients, and 106 healthy controls (HCs).White blood cell (WBC) count, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), IgM, IgG, C3, C4, RF, ANA, dsDNA, Sm, AnuA, aCL, anti-SSA, and anti-SSB were measured by standard laboratory techniques. SLE Disease Activity Index (SLEDAI) was evaluated accordingly. Anti-tubulin-α-1C antibody levels were significantly increased in SLE patients. Elevated anti-tubulin-α-1C were correlated with higher levels of SLEDAI, increased titers of anti-Sm antibody, and decreased titers of anti-dsDNA antibody and significantly associated with cutaneous and mucosal vasculitis and milder renal involvement. Anti-tubulin-α-1C may become a novel biomarker indicative of active vasculitis in SLE and could be applied in future clinical practice.


Anti-tubulin-α-1C Autoantibody Systemic lupus erythematosus 



This work was supported by funding from National Natural Science Foundation of China (81471601 and 81671602), Beijing Natural Science Foundation (7152150), and Beijing Nova Program (Z171100001117025).

Compliance with ethical standards

The study was approved by the Ethics Committee of Peking University People’s Hospital according to the declaration of Helsinki with the following reference number: 2015PHB219-01. All patients had been informed and signed the consent for the participation in this study.



Supplementary material

10067_2018_4024_MOESM1_ESM.pdf (187 kb)
ESM 1 (PDF 187 kb).


  1. 1.
    Liu Z, Davidson A (2012) Taming lupus—a new understanding of pathogenesis is leading to clinical advances. Nat Med 18(6):871–882. CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Sherer Y, Gorstein A, Fritzler MJ, Shoenfeld Y (2004) Autoantibody explosion in systemic lupus erythematosus: more than 100 different antibodies found in SLE patients. Semin Arthritis Rheum 34(2):501–537. CrossRefPubMedGoogle Scholar
  3. 3.
    Migliorini P, Baldini C, Rocchi V, Bombardieri S (2005) Anti-Sm and anti-RNP antibodies. Autoimmunity 38(1):47–54. CrossRefPubMedGoogle Scholar
  4. 4.
    ter Borg EJ, Horst G, Hummel EJ, Limburg PC, Kallenberg CG (1990) Measurement of increases in anti-double-stranded DNA antibody levels as a predictor of disease exacerbation in systemic lupus erythematosus. A long-term, prospective study. Arthritis Rheum 33(5):634–643. CrossRefPubMedGoogle Scholar
  5. 5.
    Popovic K, Brauner S, Ek M, Wahren-Herlenius M, Nyberg F (2007) Fine specificity of the Ro/SSA autoantibody response in relation to serological and clinical findings in 96 patients with self-reported cutaneous symptoms induced by the sun. Lupus 16:10–17CrossRefPubMedGoogle Scholar
  6. 6.
    Ibrahim A. Al-Homood(2012) Thrombosis in systemic lupus erythematosus: a review article. ISRNRheumatol 2012: 428269Google Scholar
  7. 7.
    Doyle M (2006) Vasculitis associated with connective tissue disorders. Curr Rheumatol Rep 8:312–316CrossRefPubMedGoogle Scholar
  8. 8.
    Pyrpasopoulou A, Chatzimichailidou S, Aslanadis S (2012) Vascular disease in systemic lupus erythematosus. Autoimmune Dis 2012:876456Google Scholar
  9. 9.
    Janke C1 (2014) The tubulin code: molecular components, readout mechanisms, and functions. J Cell Biol 206:461–472CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D et al (2014) Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics 13(2):397–406CrossRefPubMedGoogle Scholar
  11. 11.
    Trudie A. Goers, Sabarinathan Ramachandran, Aviva Aloush, et al. (2008 )De novo production of K-α1 tubulin specific antibodies: role in chronic lung allograft rejection. J Immunol 180: 4487–4494Google Scholar
  12. 12.
    Hachema RR, Tiriveedhib V, Pattersonc G et al (2012) Antibodies to K-a 1 tubulin and collagen V are associated with chronic rejection after lung transplantation. Am J Transplant 12(8):2164–2171. CrossRefGoogle Scholar
  13. 13.
    Cong Y, Eric Gershwin M, Chang C (2014) Diagnostic criteria for systemic lupus erythematosus: a critical review. J Autoimmun 48–49:10–13Google Scholar
  14. 14.
    Yung S, Chan TM (2015) Mechanisms of kidney injury in lupus nephritis—the role ofanti-dsDNA antibodies. Front Immunol 6:475CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Avrameas S, Guilbert B, Dighiero G (1981) Natural antibodies against tubulin, actin myoglobin, thyroglobulin, fetuin, albumin and transferrin are present in normal human sera and monoclonal immunoglobulins from multiple myeloma and Waldenström’s macroglobulinemia may express similar antibody specificities. AnnImmunol (Paris) 132C:231–236Google Scholar
  16. 16.
    Dighiero G, Guilbert B, Avrameas S (1982) Naturally occurring antibodies against nine common antigens in humans sera. II. High incidence of monoclonal Ig exhibiting antibody activity against actin and tubulin and sharing antibody specificities with natural antibodies. J Immunol 128:2788–2792PubMedGoogle Scholar
  17. 17.
    Karsenti E, Guilbert B, Bornens M, Avrameas S (1977) Anti-actin and anti-tubulin antibodies in the serum of non-immunized animals. AnnImmunol (Paris) 128:195–200Google Scholar
  18. 18.
    Kurki P, Virtanen I (1984) The detection of human antibodies against cytoskeletal components. J Immunol Methods 67:209–223CrossRefPubMedGoogle Scholar
  19. 19.
    Mead GM, Cowin P, Whitehouse JM (1980) Antitubulin antibody in healthy adults and patients with infectious mononucleosis and its relationship to smooth muscle antibody (SMA). ClinExpImmunol 39:328–336Google Scholar
  20. 20.
    Newcombe J, Gahan S, Cuzner ML (1985) Serum antibodies against central nervous system proteins in human demyelinating disease. Clin Exp Immunol 59:383–390PubMedPubMedCentralGoogle Scholar
  21. 21.
    Rousset B, Bernier-Valentin F, Poncet C, Orgiazzi J, Madec AM, Monier JC, Mornex R (1983) Anti-tubulin antibodies in autoimmune thyroid disorders. Clin Exp Immuno l52:325–332Google Scholar
  22. 22.
    Newcombe J, Gahan S, Cuzner ML (1985) Serum antibodies against central nervous system proteins in human demyelinating disease. Clin Exp Immunol 59:383–390PubMedPubMedCentralGoogle Scholar
  23. 23.
    Drenkard C, Villa AR, Reyes E, Abello M, Alarcón-Segovia D (1997) Vasculitis in systemic lupus erythematosus. Lupus 6(3):235–242. CrossRefPubMedGoogle Scholar
  24. 24.
    Ramos-Casals M, Nardi N, Lagrutta M et al (2006) Vasculitis in systemic lupus erythematosus: prevalence and clinical characteristics in 670 patients. Medicine 85:95–104CrossRefPubMedGoogle Scholar
  25. 25.
    D'Cruz D (1998) Vasculitis in systemic lupus erythematosus. Lupus 7:270–274CrossRefPubMedGoogle Scholar
  26. 26.
    Bacon PA, Carruthers DM (1995) Vasculitis associated with connective tissue disorders. Rheum Dis Clin N Am 21:1077–1096Google Scholar
  27. 27.
    Praprotnik S, Blank M, Meroni PL et al (2001) Classification of antiendothelial cell antibodies into antibodies against microvascular and macrovascular endothelial cells. Arthritis Rheum 44:1484–1494CrossRefPubMedGoogle Scholar

Copyright information

© International League of Associations for Rheumatology (ILAR) 2018

Authors and Affiliations

  • Xiaozhen Zhao
    • 1
    • 2
  • Yongjing Cheng
    • 3
  • Yuzhou Gan
    • 1
    • 2
  • Rulin Jia
    • 1
    • 2
  • Lei Zhu
    • 1
    • 2
  • Xiaolin Sun
    • 1
    • 2
  1. 1.Department of Rheumatology and ImmunologyPeking University People’s HospitalBeijingChina
  2. 2.Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135)BeijingChina
  3. 3.Department of Rheumatology and ImmunologyBeijing HospitalBeijingChina

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