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Immunosensor for electrodetection of the C-reactive protein in serum

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Abstract

Epidemiological studies have demonstrated an association between the risk of cardiovascular events and increasing C-reactive protein (CRP) concentration. This paper reports the development of an immunosensor for the assessment of the cardiovascular process using anti-C-reactive protein antibody immobilized onto a gold-printed screen electrode. Positive and negative human sera were successfully evaluated using electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and atomic force microscopy (AFM). EIS results show that, after the incubation with positive serum for myocardial infarction, the resistance increased about two times in relation to the negative serum. A linear range from 6.25 to 50 μg mL−1 and detection limit of 0.78 μg mL−1 using DPV were obtained. The immunosensor developed for the CRP detection using gold electrode revealed efficacy and a potential use for the diagnosis and monitoring of the progression of cardiovascular diseases.

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Acknowledgments

We would like to thank teacher Abílio Borghi for the review of the English manuscript.

Funding

The authors are grateful for the financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG).

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Authors and Affiliations

  1. Faculty of Electrical Engineering, Federal University of Uberlândia, Uberlândia, Brazil

    Laíse Oliveira Resende & Adriano O. Andrade

  2. Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Brazil

    Ana Cristina Honorato de Castro & Ana Graci Brito-Madurro

  3. Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Brazil

    João Marcos Madurro

Authors
  1. Laíse Oliveira Resende
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  2. Ana Cristina Honorato de Castro
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  3. Adriano O. Andrade
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  4. João Marcos Madurro
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  5. Ana Graci Brito-Madurro
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Corresponding author

Correspondence to Ana Graci Brito-Madurro.

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The research project was approved by the Ethics Committee of UFU (number 721.990).

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Resende, L.O., de Castro, A.C.H., Andrade, A.O. et al. Immunosensor for electrodetection of the C-reactive protein in serum. J Solid State Electrochem 22, 1365–1372 (2018). https://doi.org/10.1007/s10008-017-3820-z

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  • DOI: https://doi.org/10.1007/s10008-017-3820-z

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