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Journal of Solid State Electrochemistry

, Volume 22, Issue 5, pp 1413–1419 | Cite as

Characterization of the purity of phytotherapics from secondary vegetal metabolism with small-scale coulometry applied to perillic acid

  • L. C. Norte
  • E. M. de Carvalho
  • M. R. R. Tappin
  • P. P. Borges
Original Paper
  • 109 Downloads

Abstract

The use of pure materials in chemical analysis is currently in high demand due to the need to provide reliable measurement results. Phytotherapics are natural products that contain substances of secondary vegetal metabolism. Perillic acid, a monoterpenic acid with anti-inflammatory and anticancer properties, was the phytotherapic chosen to be analyzed in this work for purity by coulometry. Due to the low availability of perillic acid on the market and its high cost, the miniaturization of the coulometric technique was fundamental for characterizing such a substance. The volume of the supporting electrolyte and the mass of the analyte were the main parameters scaled. The coulometric measurement results were obtained by small-scale coulometry by using 3.0 mg of perillic acid (scale reduction of ×100). A mean purity of (94.2 ± 0.8)% (k = 2.13, for an approximately 95% confidence level) was obtained from five replicates. These studies aim in the future to develop the production of certified reference materials from natural products.

Keywords

Characterization Small-scale coulometry Phytotherapic Perillic acid 

Notes

Acknowledgements

The author L. C. Norte acknowledges the Inmetro and the Fiocruz, where the studies are being developed.

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Copyright information

© Springer-Verlag GmbH Germany 2017
corrected publication March/2018

Authors and Affiliations

  • L. C. Norte
    • 1
  • E. M. de Carvalho
    • 1
  • M. R. R. Tappin
    • 1
  • P. P. Borges
    • 2
  1. 1.Fiocruz, FarmanguinhosRio de JaneiroBrazil
  2. 2.Chemical and Thermal Metrology Division, Electrochemistry LaboratoryNational Institute of Metrology, Quality and Technology—InmetroDuque de CaxiasBrazil

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