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Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode


Giant unilamellar liposomes of the synthetic cardiolipin 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol give chronoamperometric current peaks at a stationary mercury electrode. The signals are due to the adhesion and spreading of the liposomes on the hydrophobic mercury surface. The potential dependence shows a minimum of the peak frequency at the point of zero charge, a large maximum of peak frequency at about −0.2 V and a second, however, smaller maximum at −0.8 V. The electrochemical behaviour of the liposomes indicates phase transitions of the cardiolipin which could be also observed in differential scanning calorimetry.

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The authors acknowledge financial support from Phospholipid Research Center, Heidelberg, Germany, and by Deutsche Forschungsgemeinschaft (He 1616/14).

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Correspondence to Fritz Scholz.

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Dedicated to Nina Fjodorovna Zakharchuk on the occasion of her 75th birthday.

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Zander, S., Hermes, M., Scholz, F. et al. Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode. J Solid State Electrochem 16, 2391–2397 (2012).

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  • Cardiolipin
  • 1′,3′-Bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol
  • Liposome
  • Chronoamperometry
  • Adhesion
  • Spreading