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Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode

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Abstract

Giant unilamellar liposomes of the synthetic cardiolipin 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol give chronoamperometric current peaks at a stationary mercury electrode. The signals are due to the adhesion and spreading of the liposomes on the hydrophobic mercury surface. The potential dependence shows a minimum of the peak frequency at the point of zero charge, a large maximum of peak frequency at about −0.2 V and a second, however, smaller maximum at −0.8 V. The electrochemical behaviour of the liposomes indicates phase transitions of the cardiolipin which could be also observed in differential scanning calorimetry.

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Acknowledgments

The authors acknowledge financial support from Phospholipid Research Center, Heidelberg, Germany, and by Deutsche Forschungsgemeinschaft (He 1616/14).

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Authors and Affiliations

  1. Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Straße 4, 17487, Greifswald, Germany

    Stefan Zander, Michael Hermes & Fritz Scholz

  2. Institute of Physics, University of Greifswald, Felix-Hausdorff-Str. 6, 17487, Greifswald, Germany

    Andreas Gröning & Christiane A. Helm

  3. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany

    Doris Vollmer

  4. Institute of Medical Biochemistry and Molecular Biology, University Medicine Greifswald, Fleischmannstr. 42-44, 17475, Greifswald, Germany

    Uwe Lendeckel

  5. Institute of Clinical Chemistry and Laboratory Diagnostics, Division of Pathobiochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany

    Lorenz Schild

Authors
  1. Stefan Zander
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  2. Michael Hermes
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  3. Fritz Scholz
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  4. Andreas Gröning
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  5. Christiane A. Helm
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  6. Doris Vollmer
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  7. Uwe Lendeckel
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  8. Lorenz Schild
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Corresponding author

Correspondence to Fritz Scholz.

Additional information

Dedicated to Nina Fjodorovna Zakharchuk on the occasion of her 75th birthday.

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Zander, S., Hermes, M., Scholz, F. et al. Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode. J Solid State Electrochem 16, 2391–2397 (2012). https://doi.org/10.1007/s10008-012-1758-8

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  • DOI: https://doi.org/10.1007/s10008-012-1758-8

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