Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode

Abstract

Giant unilamellar liposomes of the synthetic cardiolipin 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol give chronoamperometric current peaks at a stationary mercury electrode. The signals are due to the adhesion and spreading of the liposomes on the hydrophobic mercury surface. The potential dependence shows a minimum of the peak frequency at the point of zero charge, a large maximum of peak frequency at about −0.2 V and a second, however, smaller maximum at −0.8 V. The electrochemical behaviour of the liposomes indicates phase transitions of the cardiolipin which could be also observed in differential scanning calorimetry.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

References

  1. 1.

    Schlame M (2008) J Lipid Res 49:1607–1620

    Article  CAS  Google Scholar 

  2. 2.

    Kates M, Syz JY, Gosser D, Haines TH (1993) Lipids 28:877–882

    Article  CAS  Google Scholar 

  3. 3.

    Hermes M, Scholz F, Härdtner C, Walther R, Schild L, Wolke C, Lendeckel U (2011) Angew Chem Int Ed 50:6872–6875

    Article  CAS  Google Scholar 

  4. 4.

    Hellberg D, Scholz F, Schauer F, Weitschies W (2002) Electrochem Commun 4:305–309

    Article  CAS  Google Scholar 

  5. 5.

    Hellberg D, Scholz F, Schubert F, Lovrić M, Omanović D, Agmo Hernández V, Thede R (2005) J Phys Chem B 109:14715–14726

    Article  CAS  Google Scholar 

  6. 6.

    Agmo Hernández V, Scholz F (2006) Langmuir 22:10723–10731

    Article  Google Scholar 

  7. 7.

    Agmo Hernández V, Scholz F (2008) Israel J Chem 48:169–184

    Article  Google Scholar 

  8. 8.

    Agmo Hernández V, Hermes M, Milchev A, Scholz F (2009) J Solid State Electrochem 13:639–649

    Article  Google Scholar 

  9. 9.

    Agmo Hernández V, Milchev A, Scholz F (2009) J Solid State Electrochem 13:1111–1114

    Article  Google Scholar 

  10. 10.

    Agmo Hernández V, Scholz F (2008) Bioelectrochemistry 74:149–156

    Article  Google Scholar 

  11. 11.

    Agmo Hernández V, Niessen J, Harnisch F, Block S, Greinacher A, Kroemer HK, Helm CA, Scholz F (2008) Bioelectrochemistry 74:210–216

    Article  Google Scholar 

  12. 12.

    Moscho A, Orwar O, Chiu DT, Modi BP, Zare RN (1996) PNAS USA 93:11443–11447

    Article  CAS  Google Scholar 

  13. 13.

    Vollmer D, Strey R, Vollmer J (1997) J Chem Phys 107:3619–3626

    Article  CAS  Google Scholar 

  14. 14.

    Rainier S, Jain MK, Ramirez F, Ioannou PV, Marecek JF, Wagner R (1979) Biochim Biophys Acta 558:187–198

    Article  CAS  Google Scholar 

  15. 15.

    Sankaram MB, Powell GL, Marsh D (1989) Biochim Biophys Acta 980:389–392

    Article  CAS  Google Scholar 

  16. 16.

    Lewis RNAH, Zweytick D, Pabst G, Lohner K, McElhaney RN (2007) Biophys J 92:3166–3177

    Article  CAS  Google Scholar 

  17. 17.

    Nagamachi E, Kariyama R, Kanemasa Y (1985) Physiol Chem Phys Med NMR 17:255–260

    CAS  Google Scholar 

  18. 18.

    Cevc G, Marsh D (1987) Phospholipid bilayers. Physical principles and models, vol 5. Wiley, New York, p 242

    Google Scholar 

  19. 19.

    Ruzic I, Pecar-Ilic J, Ivosevic DeNardis N (2010) J Electroanal Chem 642:120–126

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge financial support from Phospholipid Research Center, Heidelberg, Germany, and by Deutsche Forschungsgemeinschaft (He 1616/14).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Fritz Scholz.

Additional information

Dedicated to Nina Fjodorovna Zakharchuk on the occasion of her 75th birthday.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zander, S., Hermes, M., Scholz, F. et al. Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode. J Solid State Electrochem 16, 2391–2397 (2012). https://doi.org/10.1007/s10008-012-1758-8

Download citation

Keywords

  • Cardiolipin
  • 1′,3′-Bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol
  • Liposome
  • Chronoamperometry
  • Adhesion
  • Spreading