Journal of Solid State Electrochemistry

, Volume 16, Issue 7, pp 2391–2397 | Cite as

Membrane fluidity of tetramyristoyl cardiolipin (TMCL) liposomes studied by chronoamperometric monitoring of their adhesion and spreading at the surface of a mercury electrode

  • Stefan Zander
  • Michael Hermes
  • Fritz ScholzEmail author
  • Andreas Gröning
  • Christiane A. Helm
  • Doris Vollmer
  • Uwe Lendeckel
  • Lorenz Schild
Original Paper


Giant unilamellar liposomes of the synthetic cardiolipin 1′,3′-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol give chronoamperometric current peaks at a stationary mercury electrode. The signals are due to the adhesion and spreading of the liposomes on the hydrophobic mercury surface. The potential dependence shows a minimum of the peak frequency at the point of zero charge, a large maximum of peak frequency at about −0.2 V and a second, however, smaller maximum at −0.8 V. The electrochemical behaviour of the liposomes indicates phase transitions of the cardiolipin which could be also observed in differential scanning calorimetry.


Cardiolipin 1′,3′-Bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol Liposome Chronoamperometry Adhesion Spreading 



The authors acknowledge financial support from Phospholipid Research Center, Heidelberg, Germany, and by Deutsche Forschungsgemeinschaft (He 1616/14).


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Stefan Zander
    • 1
  • Michael Hermes
    • 1
  • Fritz Scholz
    • 1
    Email author
  • Andreas Gröning
    • 2
  • Christiane A. Helm
    • 2
  • Doris Vollmer
    • 3
  • Uwe Lendeckel
    • 4
  • Lorenz Schild
    • 5
  1. 1.Institute of BiochemistryUniversity of GreifswaldGreifswaldGermany
  2. 2.Institute of PhysicsUniversity of GreifswaldGreifswaldGermany
  3. 3.Max Planck Institute for Polymer ResearchMainzGermany
  4. 4.Institute of Medical Biochemistry and Molecular BiologyUniversity Medicine GreifswaldGreifswaldGermany
  5. 5.Institute of Clinical Chemistry and Laboratory Diagnostics, Division of PathobiochemistryOtto-von-Guericke University MagdeburgMagdeburgGermany

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