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Voltammetric determination of cysteine at a graphite electrode modified with gold nanoparticles

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Abstract

The electrochemical behaviour of cysteine (Cys) at a graphite electrode modified with gold nanoparticles (G-Aunano electrode) was studied by cyclic voltammetry. It was found that the graphite electrode-Au nanoparticles show an electrocatalytic activity towards the oxidation of Cys in 0.1 M NaOH. At 0.05 V, there is an “inverse” maximum in the cathodic voltammogram of Cys. Using a G-Aunano electrode, the dependence of the peak current of the “inverse” maximum on Cys concentration was linear in the range from 1 to 14 pM, and the detection limit was 0.6 pM. The proposed analytical method is simple, rapid and sensitive.

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References

  1. Teixeira MFS, Dockal ER, Cavalheiro ETG (2005) Sensor and Actuator B. 106:619

    Article  Google Scholar 

  2. Bald E, Glowacki R, Drzewoski J (2001) Determination by liquid chromatography of free and total cysteine in human urine in the form of its S-quinolinium derivative. J Chromotogr 913:319–329

    Article  CAS  Google Scholar 

  3. Kamidate T, Kuniya I, Watanabe H (1993) Simultaneous determination of cysteine and glutathione via use of time-resolved luminol chemiluminescence method. Chem Lett 12:2049–2052

    Article  Google Scholar 

  4. Kavlentis E (1988) Selective spectrophotometric determination of cysteine in the presence of cystine, methionine, and the remaining amino acids using the palladium (II)–DPPH complex. Microchem J 37:129–131

    Article  CAS  Google Scholar 

  5. Chrastil J (1989) Spectrophotometric determination of cysteine and cystine in peptides and proteins. Analyst 114:1133–1136

    Article  Google Scholar 

  6. Chrastil J (1990) Spectrophotometric determination of cysteine and cystine in urine. Analyst 115:1383–1384

    Article  CAS  Google Scholar 

  7. Yan JX, Kett WC et al (1998) Identification and quantitation of cysteine in proteins separated by gel electrophoresis. J Chromatogr A 813:187–200

    Article  CAS  Google Scholar 

  8. Tijssen P (1985) Practice and theory of enzyme immunoassays. Elsevier, Amsterdam

    Google Scholar 

  9. Vertelov GK, Oleenin AYu, Lisichkin GV (2004) Use of nanoparticles in the electrochemical analysis of biological samples. J Anal Chem 62:903–915

    Google Scholar 

  10. Fedurco M, Vesela V, Heyrovsky M (1993) Voltammetric reaction of the sulfide/disulfide system with oxygen at mercury electrodes. Electroanalysis 5:257–259

    Article  CAS  Google Scholar 

  11. Barroso MF, Santos T et al (2007) Cathodic behaviour of cysteine at a platinum electrode. Port Electrochim Acta 25:119–129

    Article  CAS  Google Scholar 

  12. Spataru N, Sarada BV et al (2001) Voltammetric determination of L-cysteine at conductive diamond electrodes. Anal Chem 73:514–519

    Article  CAS  Google Scholar 

  13. Fawcett W, Fedurco M, Kovacova Z (1994) Oxidation of cysteine, cysteine sulfinic acid and cysteic acid on a polycrystalline gold electrode. J Electroanal Chem 368:265–274

    Article  CAS  Google Scholar 

  14. Shaidarova LG, Ziganshina SA, Budnikov GK (2003) Electrocatalytic oxidation of cysteine and cystine at a carbon-paste electrode modified with ruthenium(IV) oxide. J Anal Chem 58:577–582

    Article  CAS  Google Scholar 

  15. Shaidarova LG, Gedmina AV, Chelnokova IA, Budnikov GK (2008) Electrocatalytic oxidation and flow-through determination of cysteine at glassy carbon electrode modified with binary system gold-iridium. J Appl Chem 81:949–954

    Google Scholar 

  16. Welch CM, Compton RG (2006) The use of nanoparticles in electroanalysis: a review. Anal Bioanal Chem 384:601–619

    Article  CAS  Google Scholar 

  17. Кorshunov AV, Perevezentseva DO, Konovchuk TV, Mironec EV (2010) Vliyanie dispersnogo sostava zolei serebra I zolota na ig elektrohimicheskuyu aktivnost//Izvestiya tomskogo politeknicheskogo universiteta 317: 6–13

  18. Korshunov AV, Heyrovsky M (2009) Dispersion of silver particles in aqueous solution visualized by polyrography/voltammetry. Electrochim Acta 54:6264–6268

    Article  CAS  Google Scholar 

  19. Safronov AY, Kashevski AV, Serisheva NV, Cherniak AS (1993) Adsorbciya na zolotom electrode serosoderjashih aminokislot I peptidov. Electrockimiya 29:858–863

    CAS  Google Scholar 

  20. Burke L, Nugent P (1997) The electrochemistry of Gold: I the redox behaviour of metal in aqueous media. Gold Bull 30:43–53

    Article  CAS  Google Scholar 

  21. Burke LD, Nugent PF (1998) The electrochemistry of gold: II the electrocatalytic behaviour of the metal in aqueous media. Gold Bull 31:39–50

    Article  CAS  Google Scholar 

  22. Burke L, Lyons M (1986) In: White R, Bockris J, Conway B (eds) Modern aspects of electrochemistry. No. 18. Plenum, New York, pp 169–182

    Google Scholar 

  23. Jansen M, Mudring A (1999) In: Schmidbaur H (ed) Gold, progress in chemistry, biochemistry and technology. Wiley, New York, pp 750–754

    Google Scholar 

  24. Brainina HZ (1988) ob obratimih pikah na polayrizacionnih krivih. Electrochemistry 13:678–680

    Google Scholar 

  25. Korshunov AV, Yosypchuk B, Heyrovsky M (2011) Voltammetry of aqueous chloroauric acid with hanging mercury drop electrode. Collect Czech Chem Comm 76:929–936

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of General and Inorganic Chemistry, Tomsk Polytechnic University, 30 Lenin street, Tomsk, Russia, 634050

    D. O. Perevezentseva

  2. Innovative Scientific-Educational Centre “Gold-Platinum”, Tomsk Polytechnic University, 30 Lenin street, Tomsk, Russia, 634050

    E. V. Gorchakov

Authors
  1. D. O. Perevezentseva
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  2. E. V. Gorchakov
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Correspondence to D. O. Perevezentseva.

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Perevezentseva, D.O., Gorchakov, E.V. Voltammetric determination of cysteine at a graphite electrode modified with gold nanoparticles. J Solid State Electrochem 16, 2405–2410 (2012). https://doi.org/10.1007/s10008-012-1727-2

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  • DOI: https://doi.org/10.1007/s10008-012-1727-2

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