Morphological and functional abnormalities of hippocampus in APC1638T/1638T mice

Abstract

In the present study, we examined morphology and function of hippocampus in the APC1638T/1638T mouse. Expression levels of the APC mRNA and protein were both identical in the hippocampus of the APC+/+ and APC1638T/1638T mice. The dentate gyrus of the APC1638T/1638T hippocampus was thicker, and has more densely-populated granule cells in the APC1638T/1638T mouse hippocampus. Immunoelectron microscopy revealed co-localization of APC with alpha-amino-3- hydroxy-5-methyl- isoxazole-4-propionate receptor (AMPA-R) and with PSD-95 at post-synapse in the APC+/+ hippocampus, while APC1638T was co-localized with neither AMPA-R nor PSD-95 in the APC1638T/1638T hippocampus. By immunoprecipitation assay, full-length APC expressed in the APC+/+ mouse was co-immunoprecipitated with AMPA-R and PSD-95. In contrast, APC1638T expressed in the APC1638T/1638T mouse was not co-immunoprecipitated with AMPA-R and PSD-95. In the hippocampal CA1 region of the APC1638T/1638T mouse, c-Fos expression after electric foot shock was decreased compared with the APC+/+ mouse. The present study showed some abnormalities on morphology of the hippocampus caused by a truncated APC (APC1638T). Also, our findings suggest that failure in APC binding to AMPA-R and PSD-95 may bring about less activities of hippocampal neurons in the APC1638T/1638T mouse.

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Acknowledgements

We thank Prof. Yamaguchi (Gifu University) for technical advices, and Dr. Wenduerma (Gifu University) for technical assistance. This study was supported in part by grants-in aid for science research from the Ministry of Education, Science, Sports and Culture of Japan to T.S. (Grant No. 18K06827) and T.O. (Grant No. 18K14843), and from the Kazato Research Foundation to T.O.

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Correspondence to Takao Senda.

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Li, C., Onouchi, T., Hirayama, M. et al. Morphological and functional abnormalities of hippocampus in APC1638T/1638T mice. Med Mol Morphol (2020). https://doi.org/10.1007/s00795-020-00257-3

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Keywords

  • APC
  • APC1638T
  • Hippocampus
  • PSD-95
  • AMPA receptor