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Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney

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Abstract

Although an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived from Porphyromonas gingivalis (PG-LPS). The mice were injected with PG-LPS at a concentration of 5 mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, the functions of five genes (Saa3, Ticam2, Reg3b, Ocxt2a, and Xcr1) were known. The upregulation of these genes was confirmed by quantitative polymerase chain reaction assay. Furthermore, we examined whether the expression of these upregulated genes were altered in endothelial cells derived from the kidney, in vitro. The mRNA expression levels of all five genes were significantly higher in the experimental group than in the controls (no LPS stimulation; *p < 0.05). In conclusion, the responses noted in the kidney may have arisen mainly from the endothelial cells. Moreover, upregulation of the expression levels of Saa3, Ticam2, Reg3b, Ocxt2a, and Xcr1 may be associated with the pathogenesis of CKD.

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Authors and Affiliations

  1. Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan

    Fumiya Harada, Tetsuro Morikawa, Daichi Hiraki, Aya Onishi, Bhoj Raj Adhikari, Koki Yoshida, Jun Sato, Michiko Nishimura & Yoshihiro Abiko

  2. School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan

    Fumiya Harada & Ching Zong Wu

  3. Division of Disease Control and Molecular Epidemiology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan

    Osamu Uehara, Seiko Toraya & Itsuo Chiba

  4. The Research Institute of Health Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan

    Rie Takai

  5. Department of Dentistry, Taipei Medical University Hospital, Taipei, Taiwan

    Ching Zong Wu

  6. Department of Dentistry, Lotung PohAi Hospital, Yilan, Taiwan

    Ching Zong Wu

Authors
  1. Fumiya Harada
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  2. Osamu Uehara
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  9. Koki Yoshida
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  12. Itsuo Chiba
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  13. Ching Zong Wu
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  14. Yoshihiro Abiko
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Correspondence to Yoshihiro Abiko.

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Harada, F., Uehara, O., Morikawa, T. et al. Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney. Med Mol Morphol 51, 156–165 (2018). https://doi.org/10.1007/s00795-018-0181-3

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  • DOI: https://doi.org/10.1007/s00795-018-0181-3

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