Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues
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Dusp22 (dual-specificity phosphatase 22) is considered to regulate various cellular processes through the regulation of protein dephosphorylation. In this study, we prepared a specific antibody against Dusp22, anti-Dusp22, and carried out expression analyses with mouse tissues and cultured cell lines. Western blotting analyses demonstrated a tissue-dependent expression profile of Dusp22 in the adult mouse, and strongly suggested the presence of isoforms with larger molecular masses. In fibroblast NIH3T3 cells, while both endogenous and Myc-tagged Dusp22 was diffusely distributed in the cytoplasm, Myc-Dusp22 was partially colocalized with actin cytoskeleton. From the obtained results, anti-Dusp22 was found to be a useful tool for biochemical and cell biological analyses of Dusp22.
KeywordsDusp22 Dual-specificity phosphatase Antibody Mouse tissues
Mitogen-activated protein kinase
c-jun N-terminal kinase
This work was supported in part by JSPS KAKENHI Grant (Grant nos. 16J06511, 23590124, 16K07211 and 17K16294), a grant-in-aid of the Practical Research Project for Rare/Intractable Diseases from Japan Agency for Medical Research and Development (AMED) (17bm0804009h0201), and Takeda Science Foundation.
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Conflict of interest
The authors declare no conflict of interest.
- 6.Schwertassek U, Buckley DA, Xu C-F et al (2010) Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis. FEBS J 277:2463–2473. https://doi.org/10.1111/j.1742-4658.2010.07661 CrossRefPubMedPubMedCentralGoogle Scholar