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Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues

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Abstract

Dusp22 (dual-specificity phosphatase 22) is considered to regulate various cellular processes through the regulation of protein dephosphorylation. In this study, we prepared a specific antibody against Dusp22, anti-Dusp22, and carried out expression analyses with mouse tissues and cultured cell lines. Western blotting analyses demonstrated a tissue-dependent expression profile of Dusp22 in the adult mouse, and strongly suggested the presence of isoforms with larger molecular masses. In fibroblast NIH3T3 cells, while both endogenous and Myc-tagged Dusp22 was diffusely distributed in the cytoplasm, Myc-Dusp22 was partially colocalized with actin cytoskeleton. From the obtained results, anti-Dusp22 was found to be a useful tool for biochemical and cell biological analyses of Dusp22.

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Abbreviations

Dusp:

Dual-specificity phosphatase

MAPK:

Mitogen-activated protein kinase

JNK:

c-jun N-terminal kinase

MKK:

MAPK kinase

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Acknowledgements

This work was supported in part by JSPS KAKENHI Grant (Grant nos. 16J06511, 23590124, 16K07211 and 17K16294), a grant-in-aid of the Practical Research Project for Rare/Intractable Diseases from Japan Agency for Medical Research and Development (AMED) (17bm0804009h0201), and Takeda Science Foundation.

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Author notes

  1. Nanako Hamada, Makoto Mizuno contributed equally to this work.

Authors and Affiliations

  1. Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, 480-0392, Japan

    Nanako Hamada, Makoto Mizuno, Ikuko Iwamoto & Koh-ichi Nagata

  2. Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, 501-1194, Japan

    Hiroyuki Tomita & Akira Hara

Authors
  1. Nanako Hamada
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  2. Makoto Mizuno
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  3. Hiroyuki Tomita
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  4. Ikuko Iwamoto
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  5. Akira Hara
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  6. Koh-ichi Nagata
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Corresponding author

Correspondence to Koh-ichi Nagata.

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Hamada, N., Mizuno, M., Tomita, H. et al. Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues. Med Mol Morphol 51, 111–117 (2018). https://doi.org/10.1007/s00795-017-0178-3

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  • DOI: https://doi.org/10.1007/s00795-017-0178-3

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