Creatine phosphate disodium salt protects against Dox-induced cardiotoxicity by increasing calumenin
Inhibiting endoplasmic reticulum stress (ERS)-induced apoptosis may be a new therapeutic target in cardiovascular diseases. Creatine phosphate disodium salt (CP) has been reported to have cardiovascular protective effect, but its effects on ERS are unknown. The aim of this study was to identify the mechanism by which CP exerts its cardioprotection in doxorubicin (Dox)-induced cardiomyocytes injury. In our study, neonatal rats cardiomyocytes (NRC) was randomly divided into control group, model group, and treatment group. The cell viability and apoptosis were detected. grp78, grp94, and calumenin of the each group were monitored. To investigate the role of calumenin, Dox-induced ERS was compared in control and down-regulated calumenin cardiomyocytes. Our results showed that CP decreased Dox-induced apoptosis and relieved ERS. We found calumenin increased in Dox-induced apoptosis with CP. ERS effector C/EBP homologous protein was down-regulated by CP and it was influenced by calumenin. CP could protect NRC by inhibiting ERS, this mechanisms may be associated with its increasing of calumenin.
KeywordsCreatine phosphate disodium salt Doxorubicin Endoplasmic reticulum stress
This work was supported by the National Natural Science Foundation of China (no. 81360587), the Natural Science Foundation of Inner Mongolia (no. 2016BS0806), Dr. Science Research Startup Funds of Inner Mongolia University for Nationalities (no. BS345), and the Mongolian medicine systems biology science and technology innovation team plan of Inner Mongolia.
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