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Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein

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Abstract

Morphological detection of cancer cells in the rabbit VX2 allograft transplantation model is often difficult in a certain region such as serosal cavity where reactive mesothelial cells mimic cancer cells and both cells share common markers such as cytokeratins. Therefore, tagging VX2 cells with a specific and sensitive marker that easily distinguishes them from other cells would be advantageous. Thus, we tried to establish a successively transplantable, enhanced green fluorescent protein (EGFP)-expressing VX2 model. Cancer cells obtained from a conventional VX2-bearing rabbit were cultured in vitro and transfected with an EGFP-encoding vector, and then successively transplanted in Healthy Japanese White rabbits (HJWRs) (n = 8). Besides, conventional VX2 cells were transplanted in other HJWRs (n = 8). Clinicopathological comparison analyses were performed between the two groups. The success rate of transplantation was 100 % for both groups. The sensitivity and specificity of EGFP for immunohistochemical detection of VX2 cells were 84.3 and 100 %, respectively. No significant differences in cancer cell morphology, tumor size (P = 0.742), Ki-67 labeling index (P = 0.878), or survival rate (P = 0.592) were observed between the two. VX2 cells can be genetically altered, visualized by EGFP, and successively transplanted without significant alteration of morphological and biological properties compared to those of the conventional model.

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Acknowledgments

This research was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 20790993 and No. 24590242 to H. Oshiro, No. 24700501 to T. Nagai), Exploratory Research from the Yokohama Foundation for Advancement of Medical Science (to H. Oshiro), Medical Research from Tokyo Medical University Cancer Center (to H. Oshiro).

Conflict of interest

We declare that we have no conflict of interest regarding this study.

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Authors and Affiliations

  1. Department of Pathology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan

    Hisashi Oshiro, Kiyotaka Nagahama, Yoji Nagashima, Ichiro Aoki, Shoji Yamanaka, Ayumi Murakami, Kenichi Ohashi & Koji Okudela

  2. Department of Orthopaedic Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan

    Hidenobu Fukumura

  3. Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan

    Itaru Sato & Kei Sugiura

  4. Department of Plastic and Reconstructive Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan

    Jiro Maegawa

  5. Department of Clinical Oncology and Breast Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan

    Takashi Chishima & Yasushi Ichikawa

  6. Department of Cardiovascular Research Institute, Yokohama City University Graduate School of Medicine, Yokohama, Japan

    Yoshihiro Ishikawa

  7. Department of Pathology, Tokyo Medical University Hospital, Tokyo, Japan

    Hisashi Oshiro, Hiroaki Iobe, Emi Okiyama, Toshitaka Nagao, Takeshi Nagai & Masaharu Nomura

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  1. Hisashi Oshiro
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  2. Hidenobu Fukumura
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  8. Toshitaka Nagao
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  9. Yoji Nagashima
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  10. Ichiro Aoki
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  11. Shoji Yamanaka
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  13. Jiro Maegawa
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  14. Takashi Chishima
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  15. Yasushi Ichikawa
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  16. Yoshihiro Ishikawa
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  17. Takeshi Nagai
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  18. Masaharu Nomura
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  19. Kenichi Ohashi
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Correspondence to Hisashi Oshiro.

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Oshiro, H., Fukumura, H., Nagahama, K. et al. Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein. Med Mol Morphol 48, 13–23 (2015). https://doi.org/10.1007/s00795-014-0071-2

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  • DOI: https://doi.org/10.1007/s00795-014-0071-2

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