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High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals

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Abstract

The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions and involved in catabolism of toxic aromatic organic compounds were identified. P. aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100 mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200 mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.

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Abbreviations

MALDI-TOF:

Matrix-assisted laser-desorption/ionization time-of-flight

(U) HPLC:

(ultra) High-performance liquid chromatography

MS:

Mass spectrometry

NCAIM:

National Collection of Agricultural and Industrial Microorganisms

ISS WDCM:

Institute of Soil Science World Data Center for Microorganisms

MIGS:

The minimum information about a genome sequence

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Acknowledgements

This research was supported by the JGI, Project CSP 741 and the Ministry of Science and Technological Development of Serbia, Project III43004. The work conducted by the US Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the US Department of Energy under Contract No. DE-AC02-05CH11231.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Lidija Izrael-Živković & Ivanka Karadžić

  2. Faculty of Chemistry, University of Belgrade, Belgrade, Serbia

    Vladimir Beškoski & Miroslav M. Vrvić

  3. Faculty of Applied Ecology Futura, Singidunum University, Belgrade, Serbia

    Milena Rikalović

  4. RuđerBošković Institute, Zagreb, Croatia

    Snježana Kazazić

  5. DOE Joint Genome Institute, Walnut Creek, CA, USA

    Nicole Shapiro & Tanja Woyke

  6. Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

    Gordana Gojgić-Cvijović

  7. Department of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Nela Maksimović

Authors
  1. Lidija Izrael-Živković
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  2. Vladimir Beškoski
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  3. Milena Rikalović
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  4. Snježana Kazazić
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  5. Nicole Shapiro
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  6. Tanja Woyke
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  8. Miroslav M. Vrvić
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  9. Nela Maksimović
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  10. Ivanka Karadžić
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Correspondence to Ivanka Karadžić.

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Communicated by A. Driessen.

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Izrael-Živković, L., Beškoski, V., Rikalović, M. et al. High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals. Extremophiles 23, 399–405 (2019). https://doi.org/10.1007/s00792-019-01092-w

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