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Antimicrobial surface processing of polymethyl methacrylate denture base resin using a novel silica-based coating technology

Abstract

Objectives

This study investigated the surface characteristics of denture base resin coatings prepared using a novel silica-based film containing hinokitiol and assessed the effect of this coating on Candida albicans adhesion and growth.

Methods

Silica-based coating solutions (control solution; CS) and CS containing hinokitiol (CS-H) were prepared. C. albicans biofilm formed on denture base specimens coated with each solution and these uncoated specimens (control) were analyzed using colony-forming unit (CFU) assay, fluorescence microscopy, and scanning electron microscopy (SEM). Specimen surfaces were analyzed by measuring the surface roughness and wettability and with Fourier-transform infrared (FT-IR) and proton nuclear magnetic resonance (1H NMR). Stability of coated specimens was assessed via immersion in water for 1 week for each group (control-1w, CS-1w, and CS-H-1w) followed by CFU assay, measurement of surface roughness and wettability, and FT-IR.

Results

CS-H and CS-H-1w contained significantly lower CFUs than those present in the control and control-1w, which was also confirmed via SEM. Fluorescence microscopy from the CS-H group identified several dead cells. The values of surface roughness from coating groups were significantly less than those from the control and control-1w. The surface wettability from all coating groups exhibited high hydrophobicity. FT-IR analyses demonstrated that specimens were successfully coated, and 1H NMR analyses showed that hinokitiol was incorporated inside CS-H.

Conclusions

A silica-based denture coating that incorporates hinokitiol inhibits C. albicans growth on denture.

Clinical relevance

We provide a novel antifungal denture coating which can be helpful for the treatment of denture stomatitis.

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Acknowledgements

FT-IR spectra measurements and direct deuteration reactions were conducted at the User Experiment Preparation Lab III (CROSS). 1H NMR spectroscopy experiments were conducted at the Deuteration Laboratory in J-PARC MLF.

Funding

This work was supported by JSPS KAKENHI, grant numbers 20K18649 and 20K18742.

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Authors and Affiliations

Authors

Contributions

Chiaki Tsutsumi-Arai wrote the main manuscript text and prepared Figs. 1, 2, 3 and 4. Kazuhiro Akutsu-Suyama prepared Figs. 5 and 6. All authors reviewed the manuscript.

Conceptualization: Chiaki Tsutsumi-Arai, Zenji Hiroi, Mitsuhiro Shibayama, Kazuhito Satomura; methodology: Chiaki Tsutsumi-Arai, Kazuhiro Akutsu-Suyama; formal analysis and investigation: Chiaki Tsutsumi-Arai, Chika Terada-Ito, Kazuhiro Akutsu-Suyama; writing—original draft preparation: Chiaki Tsutsumi-Arai; writing—review and editing: Kazuhito Satomura, Mitsuhiro Shibayama; funding acquisition: Chiaki Tsutsumi-Arai, Chika Terada-Ito; resources: Yoko Iwamiya; supervision: Kazuhito Satomura.

Corresponding author

Correspondence to Chiaki Tsutsumi-Arai.

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Tsutsumi-Arai, C., Akutsu-Suyama, K., Iwamiya, Y. et al. Antimicrobial surface processing of polymethyl methacrylate denture base resin using a novel silica-based coating technology. Clin Oral Invest (2022). https://doi.org/10.1007/s00784-022-04670-z

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  • DOI: https://doi.org/10.1007/s00784-022-04670-z

Keywords

  • Silica-resin coating
  • Hinokitiol
  • C. albicans
  • Denture
  • Denture stomatitis