Alveolar crest contour changes after guided bone regeneration using different biomaterials: an experimental in vivo investigation

Abstract

Objective

To evaluate the changes in alveolar contour after guided bone regeneration (GBR) with two different combinations of biomaterials in dehiscence defects around implants.

Material and methods

Chronic alveolar ridge defects were created bilaterally in the mandible of eight Beagle dogs. Once implants were placed, three treatment groups were randomly allocated to each peri-implant dehiscence defect: (i) test group received a bone substitute composed of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) covered by a cross-linked collagen membrane, (ii) positive control group with placement of deproteinized bovine bone mineral (DBBM) plus a porcine natural collagen membrane, and (iii) a negative control with no treatment. Two healing periods (8 and 16 weeks) were evaluated. Dental casts were optically scanned, the obtained files were uploaded into an image analysis software and superimposed to evaluate the linear changes.

Results

In both healing periods, the gains in linear contours were higher in the test group and at the intermediate level (3 mm below the gingival margin). While at 8 weeks, no significant differences were found between the groups; at 16 weeks, the test and positive control groups demonstrated significant gains in contour compared with negative control.

Conclusions

GBR using different biomaterials significantly increased the buccal contours of the alveolar crest when used at dehiscence defects around dental implants.

Clinical relevance

Particulate highly porous synthetic bone substitute and a cross-linked collagen membrane demonstrated similar outcomes in terms of contour augmentation when compared to bovine xenograft (DBBM) and a collagen membrane.

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Acknowledgments

The authors acknowledge professor Ui-Won Jung for the active involvement in the surgical procedure. We also thank the veterinary doctors, Maria Carmen Calles-Vázquez and Elena Abellán, as well as the staff from the Minimally Invasive Surgery Centre, Cáceres, Spain, who so effectively took care of the experimental animals used in this investigation.

Funding

This work was partially supported through a research contract between the University Complutense of Madrid and Dentium Implants, Suwon (Korea). Support was also obtained from the ETEP (Etiology and therapeutics in Periodontal Diseases) Research Group at the Faculty of Odontology, University Complutense of Madrid (Spain).

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Correspondence to Mariano Sanz.

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This article contains data from an experimental study with animals performed at the Experimental Surgical Department of the Minimally Invasive Surgery Centre in Cáceres (Spain) after receiving approval from the Regional Ethics Committee for Animal Research (CCMIJU Reference 011/15). All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Di Raimondo, R., Sanz-Esporrín, J., Plá, R. et al. Alveolar crest contour changes after guided bone regeneration using different biomaterials: an experimental in vivo investigation. Clin Oral Invest 24, 2351–2361 (2020). https://doi.org/10.1007/s00784-019-03092-8

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Keywords

  • Guided bone regeneration
  • Synthetic bone graft
  • Collagen membrane
  • Dental implant
  • Animal model
  • Prophilometric changes

MeSH Terms

  • Bone Regeneration
  • Calcium Phosphates
  • Membranes
  • Biocompatible Materials
  • Dental Implants
  • Animal Model
  • Alveolar Bone Loss