Effects of two disinfection/sterilization methods for dentin specimens on dentin permeability
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To investigate the effects of two disinfection/sterilization methods on the permeability of dentin specimens.
Materials and methods
Forty intact human third molars were freshly extracted and cut, close to the pulp chamber, into dentin disks with a 500-μm thickness. The disks were randomized (n = 20 each) into a 70% ethanol group (acid-etched dentin disks soaked in 70% ethanol for 15 min) and a steam autoclaving group (acid-etched dentin disks autoclaved for 25 min). The permeability (Lp) of each dentin disk was measured before and after either treatment using a hydraulic device, and intra- and inter-group differences in values before and after treatment were analyzed using t tests. Field emission scanning electron microscopy (FE-SEM) micrographs of the dentin surface were acquired and examined. FE-SEM samples were prepared using the critical point drying (CPD) method.
Immersion in 70% ethanol increased the Lp values of dentin specimens by 17%, which was not statistically significant. Steam autoclaving significantly reduced dentin permeability by 66% because the dentin collagen mesh became compact and collapsed, as detected by FE-SEM.
The disinfection of acid-etched dentin disks using 70% ethanol for 15 min does not significantly affect dentin permeability, whereas sterilization of acid-etched dentin disks via autoclaving significantly reduces dentin permeability.
Considering the influences of dentin permeability by disinfection/sterilization methods, the disinfection of the acid-etched dentin disks using 70% ethanol for 15 min could be used for the study related to dentin permeability, while the sterilization of autoclaving could not.
KeywordsDisinfection Sterilization Dentin permeability Dentin collagen Dentinal tubules Critical point drying
The authors would like to thank all of the donors of the extracted teeth.
This work was supported by the National Science & Technology Pillar Program during the 12th Five-Year Plan (grant number 2012BA122B03).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
For this type of study, formal consent is not required.
- 22.International Organization for Standardization (2008) ISO 7405: 2008 Dentistry—evaluation of biocompatibility of medical devices used in dentistry, ISO, Geneva. http://www.iso.org/iso/store.htm. Accessed 1 January 2017