Clinical Oral Investigations

, Volume 22, Issue 6, pp 2209–2218 | Cite as

Effect of nanostructured zirconium dioxide incorporation in an experimental adhesive resin

  • Camila Provenzi
  • Fabrício Mezzomo Collares
  • Marla Cuppini
  • Susana Maria Werner Samuel
  • Annelise Kopp Alves
  • Carlos Pérez Bergmann
  • Vicente Castelo Branco Leitune
Original Article



The aim of this study was to evaluate the influence of nanostructured zirconium dioxide incorporation in an experimental adhesive resin.


ZrO2 particles were characterized by X-ray diffraction (XRD), micro-Raman spectroscopy and Brunauer–Emmett–Teller (B.E.T). Experimental adhesive resins were formulated with 0, 0.5, 1, 4.8, and 9.1% ZrO2 in weight. The adhesives were evaluated based on degree of conversion (DC), radiopacity, softening in solvent and microtensile bond strength (μTBS) 24 h and after 1 year of aging. Mineral deposition at the hybrid layer was assessed with micro-Raman spectroscopy at the baseline and after 14 days.


XRD showed monoclinic and tetragonal phases of ZrO2.particles. B.E.T data revealed a surface area of 37.41 m2/g, and typical chemical groups were shown on the Raman spectra. The addition of ZrO2 did not influence the radiopacity. The addition of 4.8% and 9.1 wt.% ZrO2 showed higher initial hardness with increased softening in solvent (P < 0.05) and promoted mineral deposition at the dentin interface. DC was significantly increased in the group with 1% ZrO2 (P < 0.05). The μTBS test showed difference on the group with 9.1 wt.% of ZrO2, with a significant reduction after aging.


The incorporation of ZrO2 promoted mineral deposition on the adhesive interface and the addition of 1 wt.% caused a significant increase on the DC without compromising the other physicochemical characteristics, which may prove promising for the development of new dental adhesive systems.

Clinical relevance

The mineral deposition on the hybrid layer can result in a longer stability of the adhesive, thus delaying the hydrolytic degradation.


Nanostructures Zirconium dioxide Dentin bonding agents 



The authors gratefully acknowledge CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for the scholarship.


The work was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Camila Provenzi
    • 1
  • Fabrício Mezzomo Collares
    • 1
  • Marla Cuppini
    • 1
  • Susana Maria Werner Samuel
    • 1
  • Annelise Kopp Alves
    • 2
  • Carlos Pérez Bergmann
    • 2
  • Vicente Castelo Branco Leitune
    • 1
  1. 1.Dental Materials Laboratory, School of DentistryUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Laboratory of Ceramic MaterialsUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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