Clinical Oral Investigations

, Volume 22, Issue 6, pp 2161–2173 | Cite as

Biological response to self-etch adhesive after partial caries removal in rats

  • Adriana Fernandes Da Silva
  • Marcelo Rocha Marques
  • Wellington Luiz De Oliveira Da Rosa
  • Sandra Beatriz Chaves Tarquinio
  • Pedro Luiz Rosalen
  • Silvana Pereira Barros
Original Article



The purposes of this study were to evaluate a model of slow caries progression and to investigate the performance of a self-etch adhesive system for partial caries removal.

Materials and methods

Rat molars were infected with Streptococcus sobrinus 6715 culture. Different time points were analyzed: days 78, 85, and 95 (± 2). After this, the samples were processed for morphological analysis. Additionally, the first molars were restored with zinc oxide and eugenol (IRM™; Dentsply; Brazil) or adhesive system (Clearfil SE Bond™; Kuraray Medical; Japan) 78 days after caries induction. After, 3 or 15 days post-treatment, the animals were euthanized, and their mandibles were processed for morphological analysis, classified by means of scores, and submitted to statistical analysis. Subsequently, immunohistochemical analysis was performed for osteonectin (OSN) and transforming growth factor-ß1 (TGF-ß1) expression.


According to the caries induction model used, on day 95 greater inflammatory infiltration (p < 0.001), and more extensive degradation of secondary/primary dentin were demonstrated than on day 78 (p < 0.05). Furthermore, the restorative materials presented similar performance (p > 0.05) and proved to be fundamental to control the carious lesion. The TGF-ß1 and OSN were shown to be active during the caries process.


The slow caries lesion model was feasible for morphological analysis of the dentin-pulp complex. The self-etch adhesive system triggered no acute inflammatory infiltration or pulp necrosis, instead it seemed to stimulate early pulp repair.

Clinical relevance

Clearfil SE Bond™ applied directly on caries-affected dentin did not predispose to pulp inflammation; instead, it appeared to provide early biological benefits.


Dental caries Dental materials Adhesives Rats Transforming growth factor-ß1 Osteonectin Dental pulp capping 



The authors would like to thank Dr. Luiz André Pimenta (Dental Director at Craniofacial Center, University of North Carolina at ChapelHill/USA) and Dr. Flávio Fernando Demarco (Full Professor, Federal University of Pelotas at Pelotas/Brazil) for their invaluable support in this project.


This work was supported by a grant from the National Council for Scientific and Technological Development - Brazil (CNPq, Brazil no. 141608/2004-8) and from the São Paulo State Research Foundation (FAPESP, Brazil no. 04/00276–7).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed and all procedures performed in studies involving animals were in accordance with the ethical standards of the Institutional Committee for Ethics in Animal Research (State University of Campinas – UNICAMP), according to protocol no. 625-2, which is in agreement with the Ethical Principles for Animal Research established by the Brazilian College for Animal Experimentation (COBEA).

Informed consent

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

784_2017_2303_MOESM1_ESM.docx (84 kb)
ESM 1 (DOCX 84 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Adriana Fernandes Da Silva
    • 1
  • Marcelo Rocha Marques
    • 2
  • Wellington Luiz De Oliveira Da Rosa
    • 1
  • Sandra Beatriz Chaves Tarquinio
    • 3
  • Pedro Luiz Rosalen
    • 4
  • Silvana Pereira Barros
    • 5
  1. 1.Department of Restorative Dentistry, School of DentistryFederal University of Pelotas (UFPel)PelotasBrazil
  2. 2.Department of Morphology, School of DentistryState University of Campinas (UNICAMP)PiracicabaBrazil
  3. 3.Department of Semiology and Clinics, School of DentistryFederal University of Pelotas (UFPel)PelotasBrazil
  4. 4.Department of Physiological Sciences, School of DentistryState University of Campinas (UNICAMP)PiracicabaBrazil
  5. 5.Center for Oral and Systemic Diseases, Department of Periodontology, School of DentistryUniversity of North Carolina (UNC)Chapel HillUSA

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