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Correlation between tissue expression of microRNA-137 and CD8 in oral lichen planus

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Abstract

Objectives

Oral lichen planus (OLP) is a chronic, inflammatory condition, classified by the World Health Organization as a premalignant lesion. We performed this study to evaluate the correlation between microRNA-137 (miR-137) and CD8 oral tissue expression in OLP patients.

Materials and methods

Twenty OLP patients [classified into three groups: (a) papular, reticular, or plaque; (b) atrophic; and (c) erosive] and 20 healthy controls were subjected to biopsy of the oral mucosa. To evaluate CD8 tissue expression, we performed immunohistochemical examination, followed by immunostaining and computerized quantification. The expression of miR-137 was evaluated using real-time quantitative PCR. We used SPSS software (version 15 for windows) to perform the statistical analysis.

Results

Our analysis showed an increased tissue expression of CD8 (p < 0.01) and reduced expression of miR-137 (p < 0.001) in OLP patients, compared to the control group. Moreover, there was a statistically significant difference (p = 0.001) between OLP subgroups in terms of CD8 tissue expression [highest in erosive OLP and lowest in papular/reticular/plaque OLP]. However, these subgroups showed no significant difference (p = 0.168) in terms of miR-137 expression. A negative correlation (p < 0.05) between tissue expression of miR-137 and CD8 was noted with a varying correlation coefficient in different OLP subgroups (-0.250 in erosive OLP, -0.491 in atrophic OLP and -0.616 in papular/reticular/plaque OLP).

Conclusions

Our findings indicate reduced expression of miR-137 and a reverse correlation between tissue expression of miR-137 and CD8 in the oral mucosa of OLP patients.

Clinical relevance

Future studies should investigate the therapeutic potential of miR-137 overexpression in OLP patients.

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Authors and Affiliations

  1. Faculty of Dentistry, Aden University, Aden, Yemen

    Sana Maher Hasan Aghbari

  2. Faculty of Medicine, Ain Shams University, Ramsis st, 11591, Abbasia, Cairo, Egypt

    Abdelrahman Ibrahim Abushouk

  3. Faculty of Medicine, Cairo University, Cairo, Egypt

    Olfat Gamil Shakir

  4. Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt

    Shaimaa Omar Zayed

  5. Faculty of Medicine, Al-Azhar University, Cairo, Egypt

    Attia Attia

Authors
  1. Sana Maher Hasan Aghbari
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  2. Abdelrahman Ibrahim Abushouk
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  3. Olfat Gamil Shakir
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  4. Shaimaa Omar Zayed
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  5. Attia Attia
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Corresponding author

Correspondence to Abdelrahman Ibrahim Abushouk.

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Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

Approval was obtained from the Local Committee of Research Ethics at Cairo University, Egypt.

Informed consent

All participants gave an oral consent to undergo biopsy from the oral mucosa.

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Aghbari, S.M.H., Abushouk, A.I., Shakir, O.G. et al. Correlation between tissue expression of microRNA-137 and CD8 in oral lichen planus. Clin Oral Invest 22, 1463–1467 (2018). https://doi.org/10.1007/s00784-017-2252-6

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  • DOI: https://doi.org/10.1007/s00784-017-2252-6

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