Clinical Oral Investigations

, Volume 22, Issue 3, pp 1463–1467 | Cite as

Correlation between tissue expression of microRNA-137 and CD8 in oral lichen planus

  • Sana Maher Hasan Aghbari
  • Abdelrahman Ibrahim Abushouk
  • Olfat Gamil Shakir
  • Shaimaa Omar Zayed
  • Attia Attia
Original Article



Oral lichen planus (OLP) is a chronic, inflammatory condition, classified by the World Health Organization as a premalignant lesion. We performed this study to evaluate the correlation between microRNA-137 (miR-137) and CD8 oral tissue expression in OLP patients.

Materials and methods

Twenty OLP patients [classified into three groups: (a) papular, reticular, or plaque; (b) atrophic; and (c) erosive] and 20 healthy controls were subjected to biopsy of the oral mucosa. To evaluate CD8 tissue expression, we performed immunohistochemical examination, followed by immunostaining and computerized quantification. The expression of miR-137 was evaluated using real-time quantitative PCR. We used SPSS software (version 15 for windows) to perform the statistical analysis.


Our analysis showed an increased tissue expression of CD8 (p < 0.01) and reduced expression of miR-137 (p < 0.001) in OLP patients, compared to the control group. Moreover, there was a statistically significant difference (p = 0.001) between OLP subgroups in terms of CD8 tissue expression [highest in erosive OLP and lowest in papular/reticular/plaque OLP]. However, these subgroups showed no significant difference (p = 0.168) in terms of miR-137 expression. A negative correlation (p < 0.05) between tissue expression of miR-137 and CD8 was noted with a varying correlation coefficient in different OLP subgroups (-0.250 in erosive OLP, -0.491 in atrophic OLP and -0.616 in papular/reticular/plaque OLP).


Our findings indicate reduced expression of miR-137 and a reverse correlation between tissue expression of miR-137 and CD8 in the oral mucosa of OLP patients.

Clinical relevance

Future studies should investigate the therapeutic potential of miR-137 overexpression in OLP patients.


CD8 miR-137 Malignant transformation Oral lichen planus 


Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

Approval was obtained from the Local Committee of Research Ethics at Cairo University, Egypt.

Informed consent

All participants gave an oral consent to undergo biopsy from the oral mucosa.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Sana Maher Hasan Aghbari
    • 1
  • Abdelrahman Ibrahim Abushouk
    • 2
  • Olfat Gamil Shakir
    • 3
  • Shaimaa Omar Zayed
    • 4
  • Attia Attia
    • 5
  1. 1.Faculty of DentistryAden UniversityAdenYemen
  2. 2.Faculty of Medicine, Ain Shams UniversityCairoEgypt
  3. 3.Faculty of MedicineCairo UniversityCairoEgypt
  4. 4.Faculty of Oral and Dental MedicineCairo UniversityCairoEgypt
  5. 5.Faculty of MedicineAl-Azhar UniversityCairoEgypt

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