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Clinical Oral Investigations

, Volume 22, Issue 3, pp 1477–1486 | Cite as

MAGE-A11 expression contributes to cisplatin resistance in head and neck cancer

  • Stefan Hartmann
  • Leonie Zwick
  • Mario J. J. Scheurer
  • Andreas R. Fuchs
  • Roman C. Brands
  • Axel Seher
  • Hartmut Böhm
  • Alexander C. Kübler
  • Urs D. A. Müller-Richter
Original Article

Abstract

Objective

The objective of this study is to investigate the roles of melanoma-associated antigens (MAGEs) in the cisplatin treatment of head and neck cancer.

Materials and methods

We assessed the efficacy of cisplatin in a set of four head and neck cancer cell lines using a crystal violet assay. The MAGE-A expression in all cell lines was measured with RT-qPCR. The correlation between MAGE-A expression and cisplatin efficacy was investigated using Spearman’s correlation analysis. Furthermore, we established a cell line with stable overexpression of MAGE-A11 and determined influence on proliferation, cisplatin efficacy and cell apoptosis. In this cell line, the effects of cisplatin were assessed using either crystal violet assays or flow cytometry (Annexin V).

Results

For MAGE-A11, we observed the highest correlation (r = 1.000, p = 0.0417) with low cisplatin efficacy. Stable overexpression of MAGE-A11 resulted in no changes in proliferation, but in lower cisplatin cytotoxicity and lower rates of apoptosis. Also, mouse double minute 2 homolog (MDM2) expression was induced by MAGE-A11 overexpression.

Conclusion

We provide evidence that MAGE-A11 expression contributes to cisplatin resistance in head and neck cancer.

Clinical relevance

Our study underscores the negative predictive role of MAGE-A11 expression in head and neck cancer.

Keywords

Mage-A11 Melanoma-associated antigens Head and neck cancer Cisplatin 

Notes

Compliance with ethical standards

Conflict of interest

Stefan Hartmann declares that he has no conflict of interest. Leonie Zwick declares that she has no conflict of interest. Mario Scheurer declares that he has no conflict of interest.

Andreas Fuchs declares that he has no conflict of interest. Roman Brands declares that he has no conflict of interest. Axel Seher declares that he has no conflict of interest. Hartmut Böhm declares that he has no conflict of interest. Alexander Kübler declares that he has no conflict of interest. Urs Müller-Richter declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

For this type of study, formal consent is not required

Supplementary material

784_2017_2242_MOESM1_ESM.docx (14 kb)
Supplemental 1 (DOCX 13 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Stefan Hartmann
    • 1
    • 2
  • Leonie Zwick
    • 1
  • Mario J. J. Scheurer
    • 1
  • Andreas R. Fuchs
    • 1
  • Roman C. Brands
    • 1
    • 3
  • Axel Seher
    • 1
  • Hartmut Böhm
    • 1
  • Alexander C. Kübler
    • 1
  • Urs D. A. Müller-Richter
    • 1
  1. 1.Department of Oral and Maxillofacial Plastic SurgeryUniversity Hospital WürzburgWürzburgGermany
  2. 2.Interdisciplinary Center for Clinical ResearchUniversity Hospital WürzburgWürzburgGermany
  3. 3.Comprehensive Cancer Center MainfrankenUniversity Hospital WürzburgWürzburgGermany

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