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Periodontal pathogenic bacteria and aMMP-8 findings depending on periodontal conditions of patients before and after liver transplantation

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Abstract

Background

The aim of this single-center cross-sectional study was to detect the prevalence of selected periodontal pathogenic bacteria and active matrix metalloproteinase-8 (aMMP-8) level in patients before (preLTx) and after liver transplantation (postLTx).

Methods

Periodontal pocket depth (PPD) and clinical attachment loss (CAL) were assessed. Subgingival biofilm samples were analyzed using polymerase chain reaction (PCR) to detect 11 common periodontal pathogens. Gingival crevicular fluid (GCF) samples were analyzed with enzyme-linked immunosorbent assay (ELISA) to determine aMMP-8 level and assigned to a scoring system: score 0: 0–8 ng/ml, score 1: 8–20 ng/ml, and score 2: >20 ng/ml. The following were used for the statistical analysis: t test, Mann-Whitney U test, Fishers test (α = 5 %).

Results

In total, 110 patients (preLTx: n = 35, postLTx: n = 75) could be included in the study. Periodontal findings were not significantly different between groups. In microbiological analysis, a significantly higher prevalence of Campylobacter rectus in preLTx group was detected (p = 0.03). Significantly more patients with score 0 in postLTx group (p = 0.024) and significantly more patients with score 1 in preLTx group were found (p = 0.004). Furthermore, aMMP-8 concentrations for patients with moderate periodontitis were significantly lower in postLTx group compared to preLTx group (p = 0.045). Additionally, in postLTx group, aMMP-8 concentration was significantly higher in patients with severe periodontitis compared to those with no/mild periodontitis (p = 0.016).

Conclusion

LTx appears to affect aMMP-8 level, but not bacterial findings in patients after LTx.

Clinical relevance

Determination of aMMP-8 level in patients after LTx with immunosuppressive medication might lead to wrong interpretation of the results.

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Acknowledgments

The authors thank Mrs. M. Hoch for the support in the laboratory microbiological and aMMP-8 analysis.

Author information

Author notes

  1. Jan E. Slotta

    Present address: Department of General and Visceral Surgery, St. Ingbert County Hospital, St. Ingbert, Germany

  2. Otto Kollmar

    Present address: Department of General and Visceral Surgery, HELIOS Dr. Horst Schmidt-Kliniken, Wiesbaden, Germany

Authors and Affiliations

  1. Department of Cariology, Endodontology and Periodontology, University of Leipzig, Leipzig, Germany

    Dirk Ziebolz & Gerhard Schmalz

  2. Department of General, Visceral and Pediatric Surgery, University Medical Center Göttingen, Göttingen, Germany

    Anne Kauffels, Jan E. Slotta & Otto Kollmar

  3. Department of Preventive Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Göttingen, Germany

    Florian Widmer, Katja Widmer & Rainer F. Mausberg

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  1. Dirk Ziebolz
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Corresponding author

Correspondence to Dirk Ziebolz.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

The work was not funded.

Ethical approval

The study was reviewed and approved by the Ethics Committee of the University Medical Center in Goettingen, Germany (No. 43/9/07).

Informed consent

Patients were informed verbally and in writing about the study and gave written informed consent.

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Ziebolz, D., Schmalz, G., Kauffels, A. et al. Periodontal pathogenic bacteria and aMMP-8 findings depending on periodontal conditions of patients before and after liver transplantation. Clin Oral Invest 21, 745–752 (2017). https://doi.org/10.1007/s00784-016-1821-4

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  • DOI: https://doi.org/10.1007/s00784-016-1821-4

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