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Green synthesis of copper oxide nanoparticles using sinapic acid: an underpinning step towards antiangiogenic therapy for breast cancer

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Abstract

Synthesis of copper oxide nanoparticles without any chemical reductant is always a challenging methodology for biological studies. In this study, sinapic acid, a phytochemical, is used for the synthesis of stable copper oxide nanoparticles. The as-synthesized nanoparticles were characterized thoroughly using UV–Visible, IR spectroscopy, Transmission Electron Microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). Nanoparticles collected during different time intervals of synthesis (60,120 and 180 min) were subjected for analysis, where the occurrence of copper oxide nanoparticles with substantial morphology was seen at 180 min. Further, nanoparticles synthesized at 120 and 180 min were studied for their potential biological applications. These copper oxide nanoparticles evinced potential cytotoxic effects on breast cancer cells, MCF7 and MDA-MB231. Supplementarily, it also exhibited anti-angiogenic effect on endothelial cells (EA.hy926), thus confirming its potential to inhibit angiogenesis in cancer.

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Acknowledgements

Dr. S. Rajalakshmi wishes to thank DST-Inspire Faculty (Grant No. DST/INSPIRE/04/2015/001945) program for the research grant. XPS spectra were acquired by Dr. Ken Wong from Advanced Materials and Process Engineering Lab (AMPEL) at University of British Columbia.

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  1. Desingh Raj Preeth and Manickaraj Shairam authors contributed equally.

Authors and Affiliations

  1. Chemical Biology and Nanobiotechnology Laboratory, AU-KBC Research Centre, Anna University, MIT Campus, Chromepet, Chennai, 600 044, India

    Desingh Raj Preeth, Manickaraj Shairam & Subramaniyam Rajalakshmi

  2. Vascular Biology Laboratory, Department of Biotechnology and AU-KBC Research Centre, Anna University, MIT Campus, Chromepet, Chennai, 600 044, India

    Natarajan Suganya & Chatterjee Suvro

  3. Department of Chemistry, The University of British Columbia, 2036 Main Hall, Vancouver, BC, V6T 1Z1, Canada

    Roshandel Hootan & Kennepohl Pierre

  4. Department of Chemistry, IIT Madras, Chennai, 600 036, India

    Ravishankar Kartik

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  1. Desingh Raj Preeth
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Raj Preeth, D., Shairam, M., Suganya, N. et al. Green synthesis of copper oxide nanoparticles using sinapic acid: an underpinning step towards antiangiogenic therapy for breast cancer. J Biol Inorg Chem 24, 633–645 (2019). https://doi.org/10.1007/s00775-019-01676-z

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